In vitro Investigation on the Biodegradability and Biocompatibility of Genipin Cross-linked Porcine Acellular Dermal Matrix with Intrinsic Fluorescence

2012 ◽  
Vol 5 (2) ◽  
pp. 344-350 ◽  
Author(s):  
Jichuan Qiu ◽  
Jianhua Li ◽  
Guancong Wang ◽  
Lin Zheng ◽  
Na Ren ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Intrinsic Fluorescence ◽  
Dermal Matrix ◽  
In Vitro Investigation ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

scholarly journals Biocompatibility Evaluation of Human and Porcine Acellular Dermal Matrix on Human Primary Gingival Fibroblasts: In Vitro Comparative Study

2021 ◽  
Author(s):  
Ehab Azab ◽  
Abdel-Rahman Youssef
Keyword(s):  
Cell Viability ◽  
Acellular Dermal Matrix ◽  
P Value ◽  
Gingival Fibroblasts ◽  
Dermal Matrix ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
Porcine Acellular Dermal Matrix ◽  
Acellular Dermal

Abstract Objective Allogeneic and xenogeneic acellular dermal matrix (ADM) grafts have been used to treat periodontal soft tissue defects. The purpose of the current study was to compare the effect of human ADM (AlloDerm) and porcine ADM (Derma) on human primary gingival fibroblasts in vitro regarding the biocompatibility test. Materials and Methods Gingival fibroblasts were obtained from healthy adult gingiva and seeded on AlloDerm or Derma ADM in 96-well plate. The control cells were grown on a surface-treated polystyrene cell-culture plate without matrix. The cells were cultured for 3, 7, and 14 days. The fibroblasts morphology was examined using inverted microscopy, and the cell viability of fibroblasts adherent to the dermal matrix was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay after 3, 7, and 14 days in culture. The data were statistically evaluated by one-way analysis of variance. p-Value of 0.05 was considered significant. Results Gingival fibroblasts adjacent to the AlloDerm and Derma matrices were healthy, attached to the well, and did not exhibit any cytopathic changes similar to control. There were no statistically significant differences in the cell viability between the gingival fibroblasts attached to Derma and AlloDerm on day 3 (p = 0.841), day 7 (p = 0.198), and day 14 (p = 0.788). Conclusion Considering this in vitro study’s limitations, both human and porcine ADM were compatible with the surrounding human primary gingival fibroblasts. No significant differences were observed in the cell viability between the gingival fibroblasts that were attached to Derma and AlloDerm.

In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures

2013 ◽  
Vol 49 (3) ◽  
pp. 371-381 ◽  
Author(s):  
A. M. Pabst ◽  
A. Happe ◽  
A. Callaway ◽  
T. Ziebart ◽  
S. I. Stratul ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Porcine Acellular Dermal Matrix ◽  
In Vivo Characterization ◽  
Acellular Dermal

Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix

2009 ◽  
Vol 610-613 ◽  
pp. 1298-1301
Author(s):  
Xiang Rong Zhang ◽  
De Wu Liu ◽  
Guang Hua Guo ◽  
Yan Peng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gradient Centrifugation ◽  
Human Mesenchymal Stem Cells ◽  
Acellular Dermal Matrix ◽  
Culture Method ◽  
Gene Encoding ◽  
Dermal Matrix ◽  
Acellular Dermal

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.

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