Detection of channel catfish virus in cage-cultured Pangasius hypophthalmus (Sauvage, 1878) in Malaysia

2013 ◽  
Vol 37 (11) ◽  
pp. 981-983 ◽  
Author(s):  
A Siti-Zahrah ◽  
M Zamri-Saad ◽  
M Firdaus-Nawi ◽  
M K Hazreen-Nita ◽  
M Nur-Nazifah
Keyword(s):  
Channel Catfish ◽  
Channel Catfish Virus

scholarly journals Channel Catfish Virus Gene 50 Encodes a Secreted, Mucin-like Glycoprotein

Virology ◽  
1999 ◽  
Vol 257 (1) ◽  
pp. 220-227 ◽  
Author(s):  
Nathalie Vanderheijden ◽  
Larry A. Hanson ◽  
Etienne Thiry ◽  
Joseph A. Martial
Keyword(s):  
Channel Catfish ◽  
Channel Catfish Virus ◽  
Virus Gene

Isolation of channel catfish virus from channel catfish, Ictalurus punctatus (Rafinesque), broodstock*

1985 ◽  
Vol 8 (6) ◽  
pp. 557-561 ◽  
Author(s):  
P. R. BOWSER ◽  
A. D. MUNSON ◽  
H. H. JARBOE ◽  
R. FRANCIS-FLOYD ◽  
P. R. WATERSTRAT
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Channel Catfish Virus

Establishment of Walking Catfish (Clarias batrachus) Cell Lines and Development of a Channel Catfish (Ictalurus punctatus) Virus Vaccine

1981 ◽  
Vol 38 (8) ◽  
pp. 925-930 ◽  
Author(s):  
Edward J. Noga ◽  
James X. Hartmann
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Vaccine Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Kidney Cell ◽  
Clarias Batrachus ◽  
Fish Cell ◽  
Wild Type ◽  
Channel Catfish Virus

Cell lines from apparently normal gill, gonad, and kidney tissue of adult walking catfish (Clarias batrachus) were established and have been subcultured 75, 80, and 95 times, respectively. The cells were propagated in a modified Ham's F-12 medium at 25 °C but are capable of growth at 37 °C. The gill and kidney cells are the first to have been established from these tissues in fish. The kidney cell line is fibroblastic, the gonad is a mixed epithelioid–fibroblastic type, and the gill is pleomorphic. All three cell lines are susceptible to channel catfish virus (CCV), exhibiting cell fusion and other marked cytopathic effects. A live attenuated vaccine strain of CCV was produced by repeated passage of a virulent strain of CCV in kidney cell cultures. The vaccine strain is less virulent than the wild-type CCV and protects fingerling catfish against challenge with wild-type virus.Key words: channel catfish virus, Clarias batrachus, fish cell cultures, vaccine

Characteristics of a Previously Unidentified Virus from Channel Catfish (Ictalurus punctatus)

1984 ◽  
Vol 41 (5) ◽  
pp. 807-811 ◽  
Author(s):  
Donald F. Amend ◽  
Terry McDowell ◽  
Ronald P. Hedrick
Keyword(s):  
Cell Lines ◽  
Crassostrea Virginica ◽  
Virus Replication ◽  
Virus Strain ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Average Diameter ◽  
Virus Infectivity ◽  
Channel Catfish Virus ◽  
The Family

A virus (strain F82-34) was isolated from channel catfish (Ictalurus punctatus) that was clearly distinct from channel catfish virus (CCV). The virus induced extensive syncytia in channel catfish ovary (CCO) cells incubated at 26 °C. The BB and CHSE-214 cell lines supported virus replication but the FHM line was refractory. Virus replication was optimal at 25 °C where, after 96 h, approximately 50% of the virus was found to be released from CCO cells. Virus infectivity was stable for 1 mo at4, 16, and 23 °C and for 5 mo at −20 and −8Q °C but was lost after 7 d at45 °C. Partially purified virions are double-shelled hexagonal capsids characteristic of reoviruses and have an average diameter of 75 nm. F82-34 was related to but distinct from two other reoviruses from fish in neutralization studies; these three viruses, and possibly a fourth from American oysters (Crassostrea virginica), appear to represent a new taxonomic group within the family Reoviridae. We therefore propose that this new virus be tentatively designated catfish reovirus (CRV).

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