Impacts of n‐acetyl cysteine on gibberellic acid‐induced hepatorenal dysfunction through modulation of pro‐inflammatory cytokines, antifibrotic and antioxidant activity

2021 ◽  
Author(s):  
Mohamed Mohamed Soliman ◽  
Adil Aldhahrani ◽  
Ahmed Gaber ◽  
Walaa F. Alsanie ◽  
Mustafa Shukry ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gibberellic Acid ◽  
Inflammatory Cytokines ◽  
Acetyl Cysteine ◽  
Pro Inflammatory Cytokines

Mitigation of IL-1β, IL-6, TNF-α, and markers of apoptosis by ursolic acid against cisplatin-induced oxidative stress and nephrotoxicity in rats

2021 ◽  
Vol 40 (12_suppl) ◽  
pp. S397-S405
Author(s):  
Pankaj Tripathi ◽  
Saeed Alshahrani
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Uric Acid ◽  
Inflammatory Cytokines ◽  
Caspase 3 ◽  
Caspase 9 ◽  
Histological Damage ◽  
Tnf Α ◽  
Mda Content ◽  
Pro Inflammatory Cytokines

Background: Ursolic acid (UA) is a natural pentacyclic triterpenoid that is known for its benefits under several pathological conditions. Cisplatin (CP) is among the most preferred chemotherapeutic agents; however, its nephrotoxicity limits its clinical utility. Purpose: This study was aimed to determine the role of UA in the reduction of CP-induced nephrotoxicity and mitigation of pro-inflammatory cytokines and apoptosis in a rat model. Methodology: Male Wistar rats were randomized into vehicle control, CP (7.5 mg/kg), UA 10 mg/kg, and CP with UA 5 and 10 mg/kg groups. Kidney and blood samples were collected for assessment of renal function, measurement of pro-inflammatory cytokines, apoptosis markers, antioxidant activity, and tissue histology. Results: CP significantly increased the levels of serum Cr, BUN, and uric acid; it also induced histological damage reflecting the pathophysiology observed during nephrotoxicity. CP has also shown its pro-oxidant activity in kidney tissue because CP decreased the levels of GSH, SOD, and CAT; it increased the lipid peroxidation as measured by MDA content. In addition, CP significantly upregulated the activity of pro-inflammatory cytokines and expression of apoptotic markers, that is, there were increased levels of IL-1β, IL-6, TNF-α, caspase-3, and caspase-9. Two weeks of continuous treatment of UA showed significant recovery against CP-induced nephrotoxicity; UA decreased the levels of Cr, BUN, and uric acid and ameliorated histological damage. UA also downregulated the activities of IL-1β, IL-6, and TNF-α as well as expression of caspase-3 and caspase-9. Furthermore, CP-induced oxidative stress that was antagonized by UA—the levels of GSH, SOD, and CAT were significantly increased while MDA content was decreased. Conclusions: UA has a protective effect against CP-induced nephrotoxicity, which may be due to its antioxidant activity and mitigation of ILβ-1, ILβ-6, TNF-α, and markers of apoptosis.

scholarly journals Short-Term of Malathion Intoxication on Lipid Peroxidation, Antioxidant Activity, and down-regulation of pro-Inflammatory Cytokines: Organophosphorus Pesticide Toxicity in Rat Model

2021 ◽  
Author(s):  
Mehran Sayadi ◽  
Mohammad Kiani ◽  
Hiva Alipanah ◽  
Seyed Mohammad Mazloomi ◽  
Roghayeh Nejati ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Inflammatory Cytokines ◽  
Organophosphorus Pesticide ◽  
Tnf Alpha ◽  
Short Term ◽  
Sod Activity ◽  
Organ Specific ◽  
Histopathological Results ◽  
Pro Inflammatory Cytokines

Abstract Malathion (MAL), a kind of organophosphate pesticides (OPs), is one of the oldest phosphoric pesticides, which used for both domestic and commercial agricultural purposes. However, it possesses adverse effects and organ-specific toxicity in relation to the heart, kidney and other vertebrate organs. The exact effects of the short-term toxicity of MAL on lipid peroxidation, antioxidant activity, and pro-inflammatory cytokines is not yet clear enough. We evaluated lipid peroxidation (MDA level), antioxidant activity [superoxide dismutase (SOD) and catalase (CAT)], TNF-alpha, and IL-1 bet in different tissues of MAL-treated Wistar rats, at doses of 50, 100, 200 and 150 mg/kg. After 14 days exposure, CAT and SOD activity, MDA level increased in most tissues. The histopathological results showed that the most sensitive tissues to MAL toxicity were liver, kidney, and heart. Also, in general, increased TNF-alpha measured as inflammatory cytokine compared to untreated rats. IL-1 beta levels showed a dual response to the toxic effects of MAL; increased in testis, kidney and lung tissues and decreased in liver, heart and blood tissues.The present findings reinforce the concept that short-term MAL toxicity can cause tissue damage while enhancing the activity of antioxidant enzymes and reducing cytokine levels.

Subacute toxicity of chlorpyrifos on histopathological damages, antioxidant activity, and pro‐inflammatory cytokines in the rat model

2022 ◽  
Author(s):  
Hiva Alipanah ◽  
Hashmieh Kabi Doraghi ◽  
Mehran Sayadi ◽  
Amene Nematollahi ◽  
Ava Soltani Hekmat ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inflammatory Cytokines ◽  
Rat Model ◽  
Subacute Toxicity ◽  
Pro Inflammatory Cytokines

Cytokine synergy, collagenases and cartilage collagen breakdown

2003 ◽  
Vol 70 ◽  
pp. 125-133 ◽  
Author(s):  
Tim E. Cawston ◽  
Jenny M. Milner ◽  
Jon B. Catterall ◽  
Andrew D. Rowan
Keyword(s):  
Matrix Metalloproteinases ◽  
Inflammatory Cytokines ◽  
Activator Protein 1 ◽  
Activator Protein ◽  
And Cartilage ◽  
Pro Inflammatory Cytokines

We have investigated proteinases that degrade cartilage collagen. We show that pro-inflammatory cytokines act synergistically with oncastatin M to promote cartilage collagen resorption by the up-regulation and activation of matrix metalloproteinases (MMPs). The precise mechanisms are not known, but involve the up-regulation of c-fos, which binds to MMP promoters at a proximal activator protein-1 (AP-1) site. This markedly up-regulates transcription and leads to higher levels of active MMP proteins.

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