Comparative response to post‐production process of two Anastrepha ludens strains: Application in the sterile insect technique

2021 ◽  
Author(s):  
José Arredondo ◽  
Juan F. Aguirre‐Medina ◽  
José S. Meza ◽  
Jorge Cancino ◽  
Francisco Díaz‐Fleischer
Keyword(s):  
Production Process ◽  
Sterile Insect Technique ◽  
Anastrepha Ludens ◽  
Comparative Response ◽  
Post Production

Pre- and Post-Copulatory Competitiveness of the Genetic Sexing Strain Tapachula-7 of Anastrepha ludens (Diptera: Tephritidae)

2020 ◽  
Vol 113 (5) ◽  
pp. 2163-2170
Author(s):  
Yair Contreras-Navarro ◽  
Diana Pérez-Staples ◽  
Dina Orozco-Dávila ◽  
Francisco Díaz-Fleischer
Keyword(s):  
Sterile Insect Technique ◽  
Copulatory Behavior ◽  
Mass Rearing ◽  
Economic Importance ◽  
Anastrepha Ludens ◽  
Sexual Performance ◽  
Field Cage ◽  
Wild Female ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Abstract Anastrepha ludens (Loew) (Diptera: Tephritidae) is an endemic pest of Mexico, attacking several fruits of economic importance. The Sterile Insect Technique (SIT), consisting of mass-rearing, irradiation and release of adults, is used to control this pest in affected areas. Currently, a genetic sexing strain (Tapachula 7, Tap-7) consisting only of A. ludens males is being released, yet we lack information on its sexual performance at the early ages when they are released and on its post copulatory behavior in terms of sperm transfer and mating inhibition. Here, sexual competitiveness at young ages and ability to inhibit female re-mating was compared between sterile Tap-7, Standard Bisexual (SB), or wild males both in laboratory and field cage conditions. Sperm stored by females mating with wild, Tap-7, or SB strain males was also compared. Six-day-old sterile Tap-7 males had low mating rates, however, by 7 d of age 80% of males had mated. Tap-7 males were just as likely as wild males to inhibit wild female re-mating. In field cages, sterile Tap-7 males mated faster than wild and sterile males from the SB strain and had comparable mating success to wild males. Females mated to sterile Tap-7 males stored more sperm than those mated to sterile SB males. Females mated to wild males stored more sperm than females mated to any other type of male. Mass-rearing had a greater effect on decreasing sperm stored by females than irradiation. We recommend continuing the release of the GSS strain of A. ludens.

scholarly journals A Novel Genetic Sexing Strain of Anastrepha Ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 499
Author(s):  
Edwin Ramírez-Santos ◽  
Pedro Rendon ◽  
Georgia Gouvi ◽  
Antigone Zacharopoulou ◽  
Kostas Bourtzis ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Sterile Insect Technique ◽  
Production Efficiency ◽  
Small Scale ◽  
Management Approach ◽  
Anastrepha Ludens ◽  
Male Mating ◽  
Pest Species ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Anastrepha ludens (Loew) is one of the most destructive insect pests damaging several fruits of economic importance. The sterile insect technique (SIT) is used under an area-wide integrated pest management approach, to suppress these pest populations. Mass rearing facilities were initially established to produce sterile males of bi-sexual strains in support of SIT. The first genetic sexing strain (GSS) for A. ludens, Tapachula-7, based on pupal color dimorphism, was a key development since the release of males-only significantly increases the SIT efficiency. In this study, we document the development of a novel pupal color-based GSS. Twelve radiation-induced translocation lines were assessed as potential GSS in terms of recombination rates and rearing efficiency at a small scale. The best one, GUA10, was cytogenetically characterized: it was shown to carry a single translocation between the Y chromosome and chromosome 2, which is known to carry the black pupae marker. This GSS was further evaluated at medium and large scales regarding its genetic stability, productivity and quality versus Tapachula-7. GUA10 presented better genetic stability, fecundity, fertility, production efficiency, flying ability, and male mating, clear indicators that GUA10 GSS can significantly improve the efficacy and cost-effectiveness of SIT applications against this pest species.

scholarly journals Polyandrous Mexican Fruit Flies: Second Male Paternity and Biological Attributes of Transgenic Strains

Insects ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 5
Author(s):  
Betzabé Verónica-Murrieta ◽  
José Salvador Meza ◽  
Martha Lucía Baena ◽  
Gerardo Alvarado-Castillo ◽  
Diana Pérez-Staples
Keyword(s):  
Sterile Insect Technique ◽  
Mass Rearing ◽  
Larval Survival ◽  
Anastrepha Ludens ◽  
Marker Genes ◽  
Fluorescent Marker ◽  
Significant Difference ◽  
Lower Ability ◽  
Biological Attributes ◽  
Transgenic Strains

Anastrepha ludens (Diptera: Tephritidae), is a damaging agricultural pest. Currently, the Sterile Insect Technique (SIT) is used as part of its control. The SIT consists of the mass-rearing, sterilization, and release of insects in target areas. Sterile males mate with wild females, and prevent them from laying fertile eggs. However, even if females mate with sterile males, they can then remate with a second male. If this second male is wild, then this could reduce the efficiency of the SIT by producing viable offspring. The amount of progeny produced by second males (P2 values) for A. ludens is unknown. Here, we evaluated the biological attributes, mating competitiveness, and the proportion of male paternity gained by the second male, using strains that carry fluorescent marker genes and can be potentially used to develop transgenic sexing strains. Furthermore, the transgenic strains were irradiated, to test their ability to induce sterility in females. We found that the 443-G strain had significantly higher larval survival than the 419-R strain. No significant difference was found between the two strains in their mating probability with wild females. We found P2 values between 67 and 74% for the 419-R and the 443-G strain, respectively. Second male sperm precedence only decreased slightly after 12 days, suggesting that sperm from the first and second male is not mixing with time, but rather the second male’s sperm prevails. Furthermore, sterile 443-G males induced significantly higher sterility in females than sterile males from the 419-R strain. The apparent lower ability of the 443-G strain to inhibit female remating should be further investigated. Knowledge of the pre and postcopulatory performance of transgenic strains will help in understanding their potential for control.

scholarly journals Garam Indonesia Berkualitas: Studi Kandungan Logam Berat Timbal (Pb) Pada Garam [The Quality of Indonesia Salt: Study of Heavy Metal Lead (Pb) Levels in the Salt]

2019 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
Nurus Samsiyah ◽  
Anita Dewi Moelyaningrum ◽  
Prehatin Trirahayu Ningrum
Keyword(s):  
Heavy Metal ◽  
Production Process ◽  
Descriptive Analysis ◽  
Raw Material ◽  
Soil Environment ◽  
Salt Production ◽  
Pb Contamination ◽  
Production Processes ◽  
Post Production

AbstrakKualitas garam sangat ditentukan oleh perlakuan dan penanganan yang diberikan pada saat pra produksi, proses produksi maupun pasca produksi. Kontaminan Pb dalam garam dapat bersumber dari lingkungan perairan laut sebagai bahan baku, tanah sebagai media / tempat produksi garam dan lingkungan udara dimana proses produksi garam berlangsung di lahan terbuka. Tujuan penelitian ini adalah untuk menganalisis kadar logam berat timbal (Pb) dalam garam di Kabupaten Pamekasan. Jenis penelitian ini adalah jenis deskriptif. Teknik pengumpulan data penelitian melalui observasi dan wawancara. Hasil observasi menunjukkan bahwa rata-rata kadar logam berat timbal (Pb) di Kabupaten Pamekasan masih di bawah batas maksimum yang telah ditentukan dalam SNI 3556-2010 dan SNI 7387-2009 yaitu <10 ppm dengan kadar Pb terendah 0,066 ppm dan tertinggi 0,162 ppm. Proses produksi garam kurang sesuai dengan standar yang telah ditetapkan. Oleh karena itu, perlunya penangangan yang baik pada pra produksi, proses produksi hingga pasca produksi pembuatan garam agar kadar Pb tidak meningkat.AbstractThe quality of salt is very determined by the treatment and handling given during the pre-production, production and post-production processes. Pb contamination in salt can be sourced from the marine environment as raw material in the process of making salt, the soil environment as a medium / place of salt production and the air environment where the salt production process takes place in an open area. The aim of research was to analyze levels of lead heavy metals (Pb) of salt in Pamekasan Regency. This research is a descriptive analysis. The techniques of data collecting of this research are observation and interview. Observations indicate that the average level of heavy metal lead (Pb) in Pamekasan regency is still below the maximum limit specified in SNI 3556-2010 and SNI 7387-2009 is <10 ppm which is the lowest grade of 0.066 ppm and grade the highest is 0.162 ppm. The salt production process is not in accordance with established standards The Therefore, the need for good handling in pre-production, production processes to post-production of salt production so that Pb levels do not increase.

Applications of Artificial Intelligence in Media and Entertainment

2021 ◽  
pp. 201-220
Author(s):  
Richard Lachman ◽  
Michael Joffe
Keyword(s):  
Artificial Intelligence ◽  
Virtual Reality ◽  
Augmented Reality ◽  
Production Process ◽  
General Public ◽  
Technological Transformation ◽  
The World ◽  
Post Production ◽  
Tremendous Impact

Emerging developments in AI will have a tremendous impact on the world of media and entertainment. While the general public is focused on entertainment-related technology such as virtual reality and augmented reality, perhaps more significant is the technological transformation of how media experiences are created. Many of the signals about how and where these technologies will affect our lives are below the surface, deeper inside the pre-production and post-production process. This chapter will survey some of the ways in which AI affects the stories we consume, issues of ethics and equity surrounding the use of the AI in media, and early signals that presage a tectonic shift in the business of content production.

The Impact of Nonlinear Editing on the Post-Production Process

SMPTE Journal ◽  
1996 ◽  
Vol 105 (10) ◽  
pp. 641-646 ◽  
Author(s):  
Michael F. Korpi ◽  
Corey P. Carbonara ◽  
Vincent E. Young
Keyword(s):  
Production Process ◽  
Post Production ◽  
The Impact
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