3-Dimensional functionalized polycaprolactone-hyaluronic acid hydrogel constructs for bone tissue engineering

2017 ◽  
Vol 44 (4) ◽  
pp. 428-437 ◽  
Author(s):  
Stephen M. Hamlet ◽  
Cedryck Vaquette ◽  
Amit Shah ◽  
Dietmar W. Hutmacher ◽  
Saso Ivanovski
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
3 Dimensional ◽  
Hyaluronic Acid Hydrogel

Gelatin/Hydroxyapatite Scaffolds: Studies on Adhesion, Growth and Differentiation of Mesenchymal Stem Cells

2010 ◽  
Vol 93-94 ◽  
pp. 121-124
Author(s):  
Nuttapon Vachiraroj ◽  
Siriporn Damrongsakkul ◽  
Sorada Kanokpanont
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Content ◽  
Population Doubling ◽  
Population Doubling Time ◽  
3 Dimensional

In this work, we developed a 3-dimensional bone tissue engineering scaffold from type B gelatin and hydroxyapatite. Two types of scaffolds, pure gelatin (pI~5) (Gel) and gelatin/hydroxyapatite (30/70 wt./wt.) (Gel/HA), were prepared from concentrated solutions (5% wt./wt.) using foaming/freeze drying method. The results SEM revealed the interconnected-homogeneous pores of Gel and Gel/HA were 121  119 and 148  83m, respectively. Hydroxyapatite improved mechanical property of the gelatin scaffolds, especially at dry state. Compressive modulus of Gel and Gel/HA scaffolds were at 118±21.68 and 510±109.08 kPa, respectively. The results on in vitro cells culture showed that Gel/HA scaffolds promoted attachment of rat’s mesenchymal stem cells (MSC) to a 1.23 folds higher than the Gel scaffolds. Population doubling time (PDT) of MSC on Gel and Gel/HA scaffolds were 51.16 and 54.89 hours, respectively. In term of osteogenic differentiation, Gel/HA scaffolds tended to enhance ALP activity and calcium content of MSC better than those of the Gel scaffold. Therefore the Gel/HA scaffolds had a potential to be applied in bone tissue engineering.

Evaluation of Alginate and Hyaluronic Acid for Their Use in Bone Tissue Engineering

2012 ◽  
Vol 7 (1) ◽  
pp. 44 ◽  
Author(s):  
M. Rubert ◽  
M. Alonso-Sande ◽  
M. Monjo ◽  
J. M. Ramis
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue

Adipogenic Mesenchymal Stem Cells and Hyaluronic Acid as a Cellular Compound for Bone Tissue Engineering

2019 ◽  
Vol 30 (3) ◽  
pp. 777-783 ◽  
Author(s):  
Daniel Goncalves Boeckel ◽  
Patrícia Sesterheim ◽  
Thiago Rodrigues Peres ◽  
Adolpho Herbert Augustin ◽  
Krista Minéia Wartchow ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Composite Hydrogel of Methacrylated Hyaluronic Acid and Fragmented Polycaprolactone Nanofiber for Osteogenic Differentiation of Adipose-Derived Stem Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 902
Author(s):  
Madhumita Patel ◽  
Won-Gun Koh
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Hyaluronic Acid ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Composite Hydrogel ◽  
Adipose Derived Stem Cells ◽  
Alizarin Red ◽  
Composite Hydrogels

Composite hydrogels with electrospun nanofibers (NFs) have recently been used to mimic the native extracellular matrix. In this study, composite hydrogels of methacrylated hyaluronic acid containing fragmented polycaprolactone NFs were used for bone tissue engineering. The composite (NF/hydrogel) was crosslinked under ultraviolet (UV) light. The incorporation of fragmented polycaprolactone NFs increased the compression modulus from 1762.5 to 3122.5 Pa. Subsequently, adipose-derived stem cells incorporated into the composite hydrogel exhibited a more stretched and elongated morphology and osteogenic differentiation in the absence of external factors. The mRNA expressions of osteogenic biomarkers, including collagen 1 (Col1), alkaline phosphatase, and runt-related transcription factor 2, were 3–5-fold higher in the composite hydrogel than in the hydrogel alone. In addition, results of the protein expression of Col1 and alizarin red staining confirmed osteogenic differentiation. These findings suggest that our composite hydrogel provides a suitable microenvironment for bone tissue engineering.

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