scholarly journals Analytical detection of influenza A(H3N2)v and other A variant viruses from the USA by rapid influenza diagnostic tests

2012 ◽  
Vol 7 (4) ◽  
pp. 491-496 ◽  
Author(s):  
Amanda Balish ◽  
Rebecca Garten ◽  
Alexander Klimov ◽  
Julie Villanueva
Keyword(s):  
Diagnostic Tests ◽  
Influenza A ◽  
The Usa ◽  
Analytical Detection

scholarly journals Development of Neuraminidase Subtype-Specific Reference Antisera by Recombinant Protein Expressed in Baculovirus

2012 ◽  
Vol 20 (2) ◽  
pp. 140-145 ◽  
Author(s):  
Kyu-Jun Lee ◽  
Jun-Gu Choi ◽  
Hyun-Mi Kang ◽  
Kwang-Il Kim ◽  
Choi-Kyu Park ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Influenza A Virus ◽  
Diagnostic Tests ◽  
Influenza A ◽  
Recombinant Baculovirus ◽  
Cross Reactivity ◽  
Specific Reference ◽  
Simple Method ◽  
Avian Influenza A Virus

ABSTRACTOutbreaks of avian influenza A virus infection, particularly the H5N1 strains that have affected birds and some humans for the past 15 years, have highlighted the need for increased surveillance and disease control. Such measures require diagnostic tests to detect and characterize the different subtypes of influenza virus. In the current study, a simple method for producing reference avian influenza virus antisera to be used in diagnostic tests was developed. Antisera of nine avian influenza A virus neuraminidases (NA) used for NA subtyping were produced using a recombinant baculovirus. The recombinant NA (rNA) proteins were expressed in Sf9 insect cells and inoculated intramuscularly into specific-pathogen-free chickens with the ISA70 adjuvant. The NA inhibition antibody titers of the rNA antiserum were in the ranges of 5 to 8 and 6 to 9 log2units after the primary and boost immunizations, respectively. The antisera were subtype specific, showing low cross-reactivity against every other NA subtype using the conventional thiobarbituric acid NA inhibition assay. These results suggest that this simple method for producing reference NA antisera without purification may be useful for the diagnosis and surveillance of influenza virus.

scholarly journals Rapid Diagnostic Tests for Identifying Avian Influenza A(H7N9) Virus in Clinical Samples

2015 ◽  
Vol 21 (1) ◽  
Author(s):  
Yu Chen ◽  
Dayan Wang ◽  
Shufa Zheng ◽  
Yuelong Shu ◽  
Wenxiang Chen ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Diagnostic Tests ◽  
Influenza A ◽  
Rapid Diagnostic Tests ◽  
Clinical Samples ◽  
H7n9 Virus

scholarly journals Influenza A Virus H5–specific Antibodies in Mute Swans (Cygnus olor) in the USA

2015 ◽  
Vol 51 (2) ◽  
pp. 523-526 ◽  
Author(s):  
Whitney M. Kistler ◽  
David E. Stallknecht ◽  
Camille Lebarbenchon ◽  
Kerri Pedersen ◽  
David R. Marks ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Specific Antibodies ◽  
Cygnus Olor ◽  
The Usa ◽  
Mute Swans

scholarly journals Performance of rapid influenza diagnostic tests (QuickVue) for Influenza A and B Infection in India

2015 ◽  
Vol 33 (5) ◽  
pp. 26 ◽  
Author(s):  
PA Koul ◽  
H Mir ◽  
MA Bhat ◽  
UH Khan ◽  
MM Khan ◽  
...  
Keyword(s):  
Diagnostic Tests ◽  
Influenza A

scholarly journals Detection of 2009 pandemic influenza A(H1N1) virus Infection in different age groups by using rapid influenza diagnostic tests

2011 ◽  
Vol 6 (3) ◽  
pp. e30-e34 ◽  
Author(s):  
Fengxiang Gao ◽  
Carol Loring ◽  
Michael Laviolette ◽  
Denise Bolton ◽  
Elizabeth R. Daly ◽  
...  
Keyword(s):  
Virus Infection ◽  
Pandemic Influenza ◽  
Diagnostic Tests ◽  
Influenza A ◽  
H1n1 Virus ◽  
Age Groups ◽  
Influenza A H1n1 ◽  
H1n1 Virus Infection

scholarly journals Characterization and Immunogenicity of Mycobacterium paratuberculosis Secreted and Cellular Proteins

1996 ◽  
Author(s):  
Herve Bercovier ◽  
Raul Barletta ◽  
Shlomo Sela
Keyword(s):  
Cellular Immunity ◽  
Diagnostic Tests ◽  
Stress Factors ◽  
Protein Antigen ◽  
Cellular Proteins ◽  
Secreted Proteins ◽  
Mycobacterium Paratuberculosis ◽  
The Usa ◽  
Acellular Vaccine

Our long-term goal is to develop an efficient acellular vaccine against paratuberculosis based on protein antigen(s). A prerequisite to achieve this goal is to analyze and characterize Mycobacterium paratuberculosis (Mpt) secreted and cellular proteins eliciting a protective immune response. In the context of this general objective, we proposed to identify, clone, produce, and characterize: the Mpt 85B antigen and other Mpt immunoreactive secreted proteins, the Mpt L7/L12 ribosomal protein and other immunoreactive cellular proteins, Mpt protein determinants involved in invasion of epithelial cells, and Mpt protein antigens specifically expressed in macrophages. Paratuberculosis is still a very serious problem in Israel and in the USA. In the USA, a recent survey evaluated that 21.6% of the dairy herd were infected with Mpt resulting in 200-250 million dollars in annual losses. Very little is known on the virulence factors and on protective antigens of Mpt. At present, the only means of controlling this disease are culling or vaccination. The current vaccines do not allow a clear differentiation between infected and vaccinated animals. Our long-term goal is to develop an efficient acellular paratuberculosis vaccine based on Mpt protein antigen(s) compatible with diagnostic tests. To achieve this goal it is necessary to analyze and characterize secreted and cellular proteins candidate for such a vaccine. Representative Mpt libraries (shuttle plasmid and phage) were constructed and used to study Mpt genes and gene products described below and will be made available to other research groups. In addition, two approaches were performed which did not yield the expected results. Mav or Mpt DNA genes that confer upon Msg or E. coli the ability to invade and/or survive within HEp-2 cells were not identified. Likewise, we were unable to characterize the 34-39 kDa induced secreted proteins induced by stress factors due to technical difficulties inherent to the complexity of the media needed to support substantial M. pt growth. We identified, isolated, sequenced five Mpt proteins and expressed four of them as recombinant proteins that allowed the study of their immunological properties in sensitized mice. The AphC protein, found to be up regulated by low iron environment, and the SOD protein are both involved in protecting mycobacteria against damage and killing by reactive oxygen (Sod) and nitrogen (AhpC) intermediates, the main bactericidal mechanisms of phagocytic cells. SOD and L7/L12 ribosomal proteins are structural proteins constitutively expressed. 85B and CFP20 are both secreted proteins. SOD, L7/L12, 85B and CFP20 were shown to induce a Th1 response in immunized mice whereas AphC was shown by others to have a similar activity. These proteins did not interfere with the DTH reaction of naturally infected cows. Cellular immunity provides protection in mycobacterial infections, therefore molecules inducing cellular immunity and preferentially a Th1 pathway will be the best candidate for the development of an acellular vaccine. The proteins characterized in this grant that induce a cell-mediated immunity and seem compatible with diagnostic tests, are good candidates for the construction of a future acellular vaccine.

scholarly journals Clinical impact of the rapid molecular detection of RSV and influenza A and B virus in the emergency room

2021 ◽  
Author(s):  
Nicolas Yin ◽  
Marc Van Nuffelen ◽  
Magali Bartiaux ◽  
Thierry Preseau ◽  
Inge Roggen ◽  
...  
Keyword(s):  
Emergency Room ◽  
Clinical Management ◽  
Diagnostic Tests ◽  
Influenza A ◽  
Point Of Care ◽  
Antibiotic Use ◽  
Rapid Diagnostic Tests ◽  
Analytical Performance ◽  
Molecular Test ◽  
The Impact

Introduction: Using respiratory viruses' rapid diagnostic tests in the emergency room (ER) could allow a better and faster clinical management. Point-of-care PCR instruments provide now results in less than 30 minutes. The objective of this study was to assess the impact of the use of the cobas® Influenza A/B & RSV Assay for use on Roche's cobas® Liat® instrument on the clinical management of ER patients. Methods: Patients (adults and children) requiring admission or suffering from an underlying condition at risk of respiratory complication were prospectively recruited in the ER of four hospitals in the Brussels region. Physician's intentions regarding admission, isolation, antibiotic and antiviral use were collected before and after performing a cobas® Influenza A/B & RSV molecular test. Additionally, a comparison of the analytical performance of this test against antigen rapid tests and viral culture was performed as well as a time-to-result evaluation. Results: PCR yielded twice more positive results than antigen rapid diagnostic tests. It allowed a decrease in the overall need for isolation and treatment by limiting the isolation of negative patients and the antibiotic use for positive patients. Meanwhile, antiviral treatments better targeted patients with a positive influenza PCR. Conclusion: The use of a rapid influenza and RSV molecular test improves the clinical management of patients admitted to the ER by providing a fast and reliable result. Their additional cost compared to antigen tests should be balanced with the benefit of their analytical performance, leading to efficient reductions in the need of isolation and antibiotic use.

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