scholarly journals Influenza A Virus H5–specific Antibodies in Mute Swans (Cygnus olor) in the USA

2015 ◽  
Vol 51 (2) ◽  
pp. 523-526 ◽  
Author(s):  
Whitney M. Kistler ◽  
David E. Stallknecht ◽  
Camille Lebarbenchon ◽  
Kerri Pedersen ◽  
David R. Marks ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Specific Antibodies ◽  
Cygnus Olor ◽  
The Usa ◽  
Mute Swans

scholarly journals The multifaceted effect of PB1-F2 specific antibodies on influenza A virus infection

Virology ◽  
2013 ◽  
Vol 447 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
I. Košík ◽  
I. Krejnusová ◽  
M. Práznovská ◽  
G. Russ
Keyword(s):  
Virus Infection ◽  
Influenza A Virus ◽  
Influenza A ◽  
Specific Antibodies ◽  
Influenza A Virus Infection

scholarly journals Virus-Coated Layer-by-Layer Colloids as a Multiplex Suspension Array for the Detection and Quantification of Virus-Specific Antibodies

2006 ◽  
Vol 52 (8) ◽  
pp. 1575-1583 ◽  
Author(s):  
Lars Toellner ◽  
Martin Fischlechner ◽  
Boris Ferko ◽  
Reingard Maria Grabherr ◽  
Edwin Donath
Keyword(s):  
Lipid Bilayer ◽  
Influenza A Virus ◽  
Influenza A ◽  
Dynamic Range ◽  
Flow Cytometric Analysis ◽  
Simultaneous Detection ◽  
Layer By Layer ◽  
Specific Antibodies ◽  
Suspension Array ◽  
Detection And Quantification

Abstract Background: Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virus-specific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding color-coded particles. Methods: We used layer-by-layer technology to establish a multiplex suspension array with distinguishable microbeads coated with authentic viral surfaces to catch and quantify virus-specific antibodies in a flow cytometric analysis. Antigenic surfaces were generated by chimeric and wild-type baculoviruses plus 2 different influenza A virus subtypes fused to a lipid bilayer surrounding distinctly colored particles. Specificity of binding of chosen antibodies and sera was detected by immunofluorescence. Results of multiplex analysis were compared with results of ELISA. Results: Titrations of virus-specific antibodies in the multiplex suspension array demonstrated specific binding to the viral surface proteins. The multiplex suspension array gave positive results for up to log 5–diluted primary antibodies with an ∼5- to 10-fold reduced dynamic range compared with the respective ELISA. Conclusions: The bead-based multiplex suspension array is customizable and easy to establish. By displaying native influenza A virus surfaces and recombinant HIV-1 epitopes, the new assay provides a tool for the detection of major viral infections in humans.

scholarly journals TGF-β-induced IL-6 prevents development of acute lung injury in influenza A virus-infected F508del CFTR-heterozygous mice

2015 ◽  
Vol 308 (11) ◽  
pp. L1136-L1144 ◽  
Author(s):  
Parker S. Woods ◽  
Mia F. Tazi ◽  
Nicholas M. Chesarino ◽  
Amal O. Amer ◽  
Ian C. Davis
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Influenza A Virus ◽  
Influenza A ◽  
Lavage Fluid ◽  
Health Concern ◽  
Transmembrane Conductance Regulator ◽  
Influenza A Viruses ◽  
Lung Dysfunction ◽  
The Usa

As the eighth leading cause of annual mortality in the USA, influenza A viruses are a major public health concern. In 20% of patients, severe influenza progresses to acute lung injury (ALI). However, pathophysiological mechanisms underlying ALI development are poorly defined. We reported that, unlike wild-type (WT) C57BL/6 controls, influenza A virus-infected mice that are heterozygous for the F508del mutation in the cystic fibrosis transmembrane conductance regulator (HETs) did not develop ALI. This effect was associated with higher IL-6 and alveolar macrophages (AMs) at 6 days postinfection (d.p.i.) in HET bronchoalveolar lavage fluid (BALF). In the present study, we found that HET AMs were an important source of IL-6 at 6 d.p.i. Infection also induced TGF-β production by HET but not WT mice at 2 d.p.i. TGF-β neutralization at 2 d.p.i. (TGF-N) significantly reduced BALF IL-6 in HETs at 6 d.p.i. Neither TGF-N nor IL-6 neutralization at 4 d.p.i. (IL-6-N) altered postinfection weight loss or viral replication in either mouse strain. However, both treatments increased influenza A virus-induced hypoxemia, pulmonary edema, and lung dysfunction in HETs to WT levels at 6 d.p.i. TGF-N and IL-6-N did not affect BALF AM and neutrophil numbers but attenuated the CXCL-1/keratinocyte chemokine response in both strains and reduced IFN-γ production in WT mice. Finally, bone marrow transfer experiments showed that HET stromal and myeloid cells are both required for protection from ALI in HETs. These findings indicate that TGF-β-dependent production of IL-6 by AMs later in infection prevents ALI development in influenza A virus-infected HET mice.

Antiviral effect of polyphenol-enriched extract of Rumex Acetosa L. against Influenza A virus

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Derksen ◽  
W Hafezi ◽  
A Hensel ◽  
J Kühn
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Antiviral Effect ◽  
Rumex Acetosa

DETECTION OF XENOBIOTIC SUBSTANCES IN MUTE SWANS’ (CYGNUS OLOR) BLOOD

2015 ◽  
Author(s):  
Jolanta STANKEVIČIŪTĖ ◽  
Solveiga Marija BARKAUSKAITĖ ◽  
Gediminas BRAZAITIS
Keyword(s):  
Animal Species ◽  
Wild Animal ◽  
Wild Populations ◽  
New Methods ◽  
Negative Results ◽  
Cygnus Olor ◽  
Ecological Scale ◽  
Literature Reviews ◽  
Baseline Information ◽  
Mute Swans

During recent years the attention towards the effects of xenobiotic substances on wild nature has been steadily increasing. Literature reviews have revealed that active hormone-disintegrating substances might affect the reproduction of some wild animal species. Research shows anomalies of reproduction and development in various animal groups such as birds, fish, invertebrates and reptiles. Species inhabiting water and its surroundings cause the highest concern. Due to insufficient baseline information it is difficult to determine the extent of the problem in these wild populations on an ecological scale. The research described in this article is the first attempt to analyse xenobiotic substances and evaluate possible accumulation of pharmaceuticals in animals higher up in the food chain in Lithuania. This research tests new methods for to analyse for xenobiotics substances, which might be used in the future. Blood samples of 7 swans were examined using liquid chromatography, however, no xenobiotics were detected. Negative results do not eliminate the necessity for further investigate of larger samples, other species or to search for non-pharmaceutical xenobiotics.

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