scholarly journals Influence of ultrasonic pretreatment on structural properties and biological activities of lupin protein hydrolysate

2022 ◽  
Author(s):  
Gbemisola J. Fadimu ◽  
Asgar Farahnaky ◽  
Harsharn Gill ◽  
Tuyen Truong
Keyword(s):  
Structural Properties ◽  
Protein Hydrolysate ◽  
Biological Activities ◽  
Ultrasonic Pretreatment ◽  
Lupin Protein

Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch

2020 ◽  
pp. 128361
Author(s):  
Tavani Rocha Camargo ◽  
Patrícia Ramos ◽  
José M. Monserrat ◽  
Carlos Prentice ◽  
Célio J.C. Fernandes ◽  
...  
Keyword(s):  
Protein Hydrolysate ◽  
Biological Activities ◽  
Fisheries Bycatch

A novel Synthesis, Chemical Characterization and Biological Activities of Metal-Leather Protein Hydrolysate Chelates

2021 ◽  
Author(s):  
Rania Jacob ◽  
Hazem Hassan ◽  
Adel Afify ◽  
Gamal Gabr
Keyword(s):  
Melting Point ◽  
Protein Hydrolysate ◽  
Biological Activities ◽  
Chemical Characterization ◽  
Antibacterial Activities ◽  
Biochemical Properties ◽  
Mineral Salts ◽  
Mineral Absorption ◽  
E Coli ◽  
Leather Processing

Abstract Leather industries covers a wide chain of production and indirectly contributes to the economic flow. The different stages used in leather processing led to produce huge solid waste volumes. Because of the great effectiveness of amino acids as naturally chelates for minerals, the present study was carried out to recycling leather waste into its protein hydrolysate by CaO hydrolysis. The Leather protein hydrolysates (LPHs) was used to prepare metal-leather protein hydrolysate chelates (Cu2+-, Zn2+-& Fe2+-LPHCs) and some of their physical properties (i.e. λ-max, FTIR spectra, color, melting point) and biochemical properties as its antibacterial activity, as well as using as micronutrient elements for plant were evaluated. Results showed that the Cu2+-LPHC gave the highest value of melting point and λ-max than other chelates. All chelates shifted the vibration bands toward a higher frequency than LPH/CaO. Metal-leather protein hydrolysate (M-LPHCs) had antibacterial activities against E. coli, B. cereus and Micrococcus spp. mostly with Zn-LPHC and Fe-LPHC. These complexes also increased the growth characteristics and mineral absorption of spinach plants in hydroponic nutrient solution than that of mineral salts (CuSO4, ZnSO4 and FeSO4). Finally, the study concluded that M-LPHCs can be used as antimicrobial agent, micronutrients for plant and support the minerals bioavailability in animals.

scholarly journals Biological Activities and Structural Properties of the Atypical Bacteriocins Mesenterocin 52B and Leucocin B-TA33a

2001 ◽  
Vol 67 (4) ◽  
pp. 1418-1422 ◽  
Author(s):  
C. Corbier ◽  
F. Krier ◽  
G. Mulliert ◽  
B. Vitoux ◽  
A.-M. Revol-Junelles
Keyword(s):  
Circular Dichroism ◽  
Structural Properties ◽  
Sequence Homology ◽  
Synthetic Peptides ◽  
Biological Activities ◽  
Modes Of Action ◽  
Amphiphilic Helix ◽  
High Sequence Homology ◽  
Circular Dichroïsm

ABSTRACT The antibacterial spectra and modes of action of synthetic peptides corresponding to mesenterocin 52B and leucocin B-TA33a greatly differ despite their high sequence homology. Circular dichroism experiments establish the capacity of each of these two peptides to partly fold into an amphiphilic helix that might be crucial for their adsorption at lipophilic-hydrophilic interfaces.

Anticoagulant Activity and Mechanism of Non-Anticoagulant Sulfated Polysaccharides

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 1208-1208 ◽  
Author(s):  
Michael Dockal ◽  
Susanne Till ◽  
Sabine Knappe ◽  
Hartmut J. Ehrlich ◽  
Friedrich Scheiflinger
Keyword(s):  
Structural Properties ◽  
Brown Algae ◽  
Biological Activities ◽  
Anticoagulant Activity ◽  
Procoagulant Activity ◽  
The Other ◽  
Sulfated Polysaccharides ◽  
Heparin Cofactor Ii ◽  
Thrombin Inhibition ◽  
Thrombin Generation Assay

Abstract Abstract 1208 Fucoidans are sulfated polysaccharides which are extracted from brown seaweeds and echinoderms and have a wide variety of biological activities. Described as non-anticoagulant polysaccharides (NASPs), they have been demonstrated to improve clotting in FVIII- and FIX-deficient plasma (Liu et al., 2006), making them good candidates for hemophilia treatment. However, fucoidans have also been extensively studied for their anticoagulant effects (Pereira et al., 1999), which usually occur at much higher concentrations than the procoagulant activity. This opens a large procoagulant window where procoagulant activities exceed anticoagulant effects. When analyzed by a global hemostatic thrombin generation assay in hemophilia plasma the onset of procoagulant activity is observed at concentrations as low as 0.01 μg/mL with optimal activity at about 1 μg/mL. Reversal of procoagulant activity is seen at concentrations higher than 10 μg/mL. Fucoidans and other sulfated polysaccharides activate different anticoagulant mechanisms depending on their structural properties. Branched fucoidans extracted from brown algae have been shown to directly inhibit thrombin, while linear fucoidan from echinoderms activates antithrombin III (ATIII) or heparin cofactor II (HCII)-mediated thrombin inhibition (Pereira et al., 1999). Sulfated galactans also have serpin-dependent and -independent anticoagulant activities (Glauser et al., 2009). In this study we analyzed fucoidans from several brown algae species for their anticoagulant properties and mode of action to identify the candidate with the best procoagulant and lowest anticoagulant activity. NASPs from several brown algae species including L. japonica (L.j.), F. vesiculosus (F.v.), and U. pinnatifida (U.p.) showed different anticoagulant activities in an activated partial thromboplastin time (aPTT) assay. U.p. fucoidan was about twice as anticoagulant as the other fucoidan preparations, increasing clotting time by 50% at a concentration of 4 μg/mL. In addition, NASPs were analyzed in ATIII- and HCII-thrombin model assays. L.j. fucoidan activated ATIII-mediated thrombin inhibition, whereas the other fucoidans showed no effect on ATIII. Fucoidans from L.j. and F.v. had a direct effect on thrombin, starting at about 10 μg/mL. By contrast, U.p. fucoidan did not directly affect thrombin. However, all preparations increased HCII-mediated thrombin inhibition at concentrations below 1 μg/mL. This suggests that HCII is the main target for the anticoagulant activity of fucoidans. Nevertheless, we observed substantial differences between the fucoidan candidates which will be correlated to structural properties. Our work describes the assessment of anticoagulant activities of a variety of fucoidan species to better understand their intertwined pro- and anticoagulant effects. This provides important mechanistic insights for the development of hemophilia therapies. Disclosures: Dockal: Baxter Innovations GmbH: Employment. Till:Baxter Innovations GmbH: Employment. Knappe:Baxter Innovations GmbH: Employment. Ehrlich:Baxter Innovations GmbH: Employment. Scheiflinger:Baxter BioScience: Employment.

scholarly journals Influence of Polysaccharides from Aloe vera (Aloe barbadensis Miller, Liliaceae) on Mechanically Induced Cough in Cats

2010 ◽  
Vol 79 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Martina Šutovská
Keyword(s):  
Chemical Composition ◽  
Structural Properties ◽  
Statistical Evaluation ◽  
Biological Activities ◽  
Aloe Vera ◽  
Aloe Barbadensis ◽  
Cough Reflex ◽  
Antitussive Activity ◽  
Nylon Fibre ◽  
Cough Suppressant

Different plants have been reported to be a source of polysaccharides that possess a great variety of biological activities, including cough suppression. The experiments were aimed on the influence of two polysaccharides isolated from the gel (A, HF1-Z) and one from the epidermis (SL-1) of Aloe vera leaves, characterized by chemical composition and structural properties, on the cough reflex. The method of mechanically induced cough was used in conscious cats. A nylon fibre directed individually into the mucous area of the laryngopharyngeal and tracheobronchial airways via endotracheal cannula elicited the cough reflex. Statistical evaluation registered cough reflex indicators (number of cough efforts - NE, cough frequency - NE min–1 intensity of cough attacks during expiration and inspiration - IA+ and IA-, intensity of maximal inspiratory and expiratory cough efforts - IME- and IME+) showed that gel polysaccharide HF1-Z possessed higher cough-suppressing activity than dropropizine, a nonnarcotic peripheral cough suppressant. Their antitussive activity was accompanied mainly with a decrease of NE, IA+ and IA- from both laryngopharyngeal and tracheobronchial mucous areas of the airways. Results of the presented study also showed minimal influence of polysaccharide HF1-Z on expectoration predictor, IME- and IME+. New pharmacodynamic property of polysaccharides isolated from Aloe vera was shown during experiments, which could be a base of their possible therapeutic use in this indication in future.

scholarly journals Anti-Colon Cancer Activity of Novel Peptides Isolated from In Vitro Digestion of Quinoa Protein in Caco-2 Cells

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 194
Author(s):  
Xin Fan ◽  
Huimin Guo ◽  
Cong Teng ◽  
Biao Zhang ◽  
Christophe Blecker ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Anticancer Activity ◽  
Protein Hydrolysate ◽  
Biological Activities ◽  
Quantitative Polymerase Chain Reaction ◽  
In Vitro Digestion ◽  
The Novel ◽  
Histone Deacetylase 1 ◽  
Liquid Chromatography Tandem Mass

Quinoa peptides are the bioactive components obtained from quinoa protein digestion, which have been proved to possess various biological activities. However, there are few studies on the anticancer activity of quinoa peptides, and the mechanism has not been clarified. In this study, the novel quinoa peptides were obtained from quinoa protein hydrolysate and identified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The anticancer activity of these peptides was predicted by PeptideRanker and evaluated using an antiproliferative assay in colon cancer Caco-2 cells. Combined with the result of histone deacetylase 1 (HDAC1) inhibitory activity assay, the highly anticancer activity peptides FHPFPR, NWFPLPR, and HYNPYFPG were screened and further investigated. Molecular docking was used to analyze the binding site between peptides and HDAC1, and results showed that three peptides were bound in the active pocket of HDAC1. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR), and Western blot showed that the expression of HDAC1, NFκB, IL-6, IL-8, Bcl-2 was significantly decreased, whereas caspase3 expression showed a remarkable evaluation. In conclusion, quinoa peptides may have the potential to protect against cancer development by inhibiting HDAC1 activity and regulating the expression of the cancer-related genes, which indicates that these peptides could be explored as functional foods to alleviate colon cancer.

Phosphorus–nitrogen compounds. Part 42. The comparative syntheses of 2-cis-4-ansa(N/O) and spiro(N/O) cyclotetraphosphazene derivatives: spectroscopic and crystallographic characterization, antituberculosis and cytotoxic activity studies

2019 ◽  
Vol 43 (18) ◽  
pp. 6856-6873 ◽  
Author(s):  
Arzu Binici ◽  
Aytuğ Okumuş ◽  
Gamze Elmas ◽  
Zeynel Kılıç ◽  
Nagehan Ramazanoğlu ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Structural Properties ◽  
Biological Activities ◽  
Nitrogen Compounds

Syntheses, structural properties and biological activities of ferrocenyl ansa- and spiro-cyclotetraphosphazenes were investigated.

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