Investigation of umami and kokumi taste-active components in bovine bone marrow extract produced during enzymatic hydrolysis and Maillard reaction

2018 ◽  
Vol 53 (11) ◽  
pp. 2465-2481 ◽  
Author(s):  
Xinru Xu ◽  
Mengchen You ◽  
Huanlu Song ◽  
Lin Gong ◽  
Wenqing Pan
Keyword(s):  
Bone Marrow ◽  
Enzymatic Hydrolysis ◽  
Maillard Reaction ◽  
Bovine Bone ◽  
Active Components

THE FATE OF BOVINE BONE MARROW STROMAL CELLS IN HYDROGELS

2008 ◽  
Vol 41 ◽  
pp. S130 ◽  
Author(s):  
Stephan Zeiter ◽  
Marije van der Werf ◽  
Keita Ito
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bovine Bone

scholarly journals Species Identification and Quantification of Bovine Bone Marrow from Non-Bovine Products using DNA and Protein Fingerprints

2021 ◽  
Author(s):  
Yize Guan ◽  
Nan Li ◽  
Tancheng Li ◽  
Liyuan Sun ◽  
Li Ming Cheng
Keyword(s):  
Bone Marrow ◽  
Multiplex Pcr ◽  
High Sensitivity ◽  
Bovine Bone ◽  
Assay Sensitivity ◽  
Conserved Sequence ◽  
Specific Pcr ◽  
Modified Method ◽  
Sds Page ◽  
Mitochondrial Cytochrome B

Abstract Bovine bone marrow is traditionally regarded as a highly nutritious food that has been widely used as a medicinal and healthy food for several decades in China. A large number of adulterated and counterfeit bone marrows from pigs and donkeys have been used in place of bovine bone marrow in commercial products, which are almost identical morphologically to species. Therefore, we explored a feasibility of fingerprinting of multiplex PCR gels combined with imaged SDS-PAGE gels of proteins. Three pairs of specific primers for bovine, pig and donkey were designed according to the conserved sequence in mitochondrial cytochrome b. The optimal reaction conditions for triple PCR were optimized. A modified method was used to extract total proteins and SDS-PAGE gels of proteins were set up. A three-fold PCR detection assay was successfully established to identify three species of bovine, pig and donkey. Three primers have good specificity and high sensitivity. Additionally, the assay sensitivity test confirmed that the extracted DNA concentration was the lowest of bovine bone marrow at 0.1 pg. The assay also showed 100% specificity. The electrophoretogram of SDS-PAGE showed distinct electrophoresis profiles among three species. The established fingerprint of multiplex PCR DNA gels combined with imaged SDS-PAGE gels of proteins has strong specificity and can be quickly and accurately used for the identification of bovine bone marrow. Rapid authentication of bovine bone marrow and differentiation from non-bovine products can be achieved using an improved SDS alkali denaturation method, species-specific PCR assay combined with imaged SDS-PAGE gels of proteins.

A new subset of small stem cells in bovine bone marrow stromal cell populations

2019 ◽  
Vol 120 (8) ◽  
pp. 13881-13892
Author(s):  
Shaohui Pan ◽  
Yu‐Chen Chen ◽  
Ning Zhao ◽  
Xiang Feng ◽  
Dan‐Dan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Populations ◽  
Bovine Bone ◽  
Marrow Stromal Cell

Bovine Bone Marrow

1963 ◽  
Vol 4 (1) ◽  
pp. 160-165 ◽  
Author(s):  
J.K.H. Wilde
Keyword(s):  
Bone Marrow ◽  
Bovine Bone
Sign in / Sign up

Export Citation Format

Share Document