Effect of thermal treatment on aroma generation from bovine bone marrow extract during enzymatic hydrolysis

2019 ◽  
Vol 43 (10) ◽  
Author(s):  
Nabila Begum ◽  
Ali Raza ◽  
Huanlu Song ◽  
Yu Zhang ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Enzymatic Hydrolysis ◽  
Thermal Treatment ◽  
Bovine Bone ◽  
Aroma Generation

THE FATE OF BOVINE BONE MARROW STROMAL CELLS IN HYDROGELS

2008 ◽  
Vol 41 ◽  
pp. S130 ◽  
Author(s):  
Stephan Zeiter ◽  
Marije van der Werf ◽  
Keita Ito
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bovine Bone

scholarly journals Species Identification and Quantification of Bovine Bone Marrow from Non-Bovine Products using DNA and Protein Fingerprints

2021 ◽  
Author(s):  
Yize Guan ◽  
Nan Li ◽  
Tancheng Li ◽  
Liyuan Sun ◽  
Li Ming Cheng
Keyword(s):  
Bone Marrow ◽  
Multiplex Pcr ◽  
High Sensitivity ◽  
Bovine Bone ◽  
Assay Sensitivity ◽  
Conserved Sequence ◽  
Specific Pcr ◽  
Modified Method ◽  
Sds Page ◽  
Mitochondrial Cytochrome B

Abstract Bovine bone marrow is traditionally regarded as a highly nutritious food that has been widely used as a medicinal and healthy food for several decades in China. A large number of adulterated and counterfeit bone marrows from pigs and donkeys have been used in place of bovine bone marrow in commercial products, which are almost identical morphologically to species. Therefore, we explored a feasibility of fingerprinting of multiplex PCR gels combined with imaged SDS-PAGE gels of proteins. Three pairs of specific primers for bovine, pig and donkey were designed according to the conserved sequence in mitochondrial cytochrome b. The optimal reaction conditions for triple PCR were optimized. A modified method was used to extract total proteins and SDS-PAGE gels of proteins were set up. A three-fold PCR detection assay was successfully established to identify three species of bovine, pig and donkey. Three primers have good specificity and high sensitivity. Additionally, the assay sensitivity test confirmed that the extracted DNA concentration was the lowest of bovine bone marrow at 0.1 pg. The assay also showed 100% specificity. The electrophoretogram of SDS-PAGE showed distinct electrophoresis profiles among three species. The established fingerprint of multiplex PCR DNA gels combined with imaged SDS-PAGE gels of proteins has strong specificity and can be quickly and accurately used for the identification of bovine bone marrow. Rapid authentication of bovine bone marrow and differentiation from non-bovine products can be achieved using an improved SDS alkali denaturation method, species-specific PCR assay combined with imaged SDS-PAGE gels of proteins.

A new subset of small stem cells in bovine bone marrow stromal cell populations

2019 ◽  
Vol 120 (8) ◽  
pp. 13881-13892
Author(s):  
Shaohui Pan ◽  
Yu‐Chen Chen ◽  
Ning Zhao ◽  
Xiang Feng ◽  
Dan‐Dan Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Cell Populations ◽  
Bovine Bone ◽  
Marrow Stromal Cell

Bovine Bone Marrow

1963 ◽  
Vol 4 (1) ◽  
pp. 160-165 ◽  
Author(s):  
J.K.H. Wilde
Keyword(s):  
Bone Marrow ◽  
Bovine Bone

182 In Vitro Derivation of Male Germ Cells from Bovine Bone Marrow-Derived Mesenchymal Stem Cells

2018 ◽  
Vol 30 (1) ◽  
pp. 231
Author(s):  
J. Cortez ◽  
J. Bahamonde ◽  
J. Palomino ◽  
M. De los Reyes ◽  
C. Torres ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Germ Cell ◽  
Mrna Expression ◽  
Male Germ Cell ◽  
Bovine Bone ◽  
Mrna Levels ◽  
Germ Cell Differentiation

During the last few years, the in vitro derivation of germ cell lineages from stem cells has emerged as an exciting new strategy for obtaining mature gametes. In vitro gamete derivation technology has potential applications as an alternative method for dissemination of elite animal genetics, production of transgenic animals, and conservation of endangered species. Germ cell differentiation and gametogenesis is a complex process and potential of different stem cell donors (i.e. SSC, ESC, iPSC) for in vitro male germ cell derivation has been inconsistent. Mesenchymal stem cells (MSC) may be suitable candidates for in vitro gamete derivation considering their (1) plasticity that is not limited to mesodermal derivatives, (2) availability of abundant tissues sources for isolation, (3) high proliferative potential, (4) simple and inexpensive isolation, and (5) high potential for cell therapy, including autologous or allogenic transplantation. The present study aimed to induce differentiation of MSC isolated from bone marrow derived from bovine male fetuses (bfMSC) into the germ cell lineage using an in vitro approach based on the exogenous effect of retinoic acid (RA) and bone morphogenetic protein 4 (BMP4). Differentiation media consisted in control media (DMEM with high glucose plus 10% fetal bovine serum, 100 IU mL−1 penicillin, 100 μg mL−1 streptomycin, and 0.25 μg mL−1amphotericin B) supplemented with RA (0.01, 0.1, or 1 µM) or BMP4 (10, 50, or 100 ng mL−1). Cell samples were obtained from differentiating and control bfMSC cultures and analysed for expression of housekeeping genes β-ACTIN and GAPDH, pluripotent genes OCT4 and NANOG, germ cell genes FRAGILLIS, STELLA, and VASA, male germ cell genes DAZL, PIWIl2, and STRA8, and meiotic biomarker SCP3 by quantitative-PCR (Q-PCR). OCT4, NANOG, and DAZL were immunodetected in undifferentiated and differentiated bfMSC using flow-cytometry analysis. The mRNA expression of DAZL was activated by RA or BMP4 supplementation, although no differences (P > 0.05) were detected among different concentrations. DAZL and NANOG mRNA levels increased (P < 0.05) from Day 7 to Day 21 during supplementation of RA (0.1 μM). In comparison, DAZL mRNA levels increased (P < 0.05) at Day 14 during supplementation of BMP4 (100 ng). OCT4 and SCP3 mRNA levels were not affected by RA or BMP4 treatments. Transcripts of FRAGILLIS, STELLA, VASA, PIWIl2, and STRA8 were not detected in control or differentiated bfMSC. Higher (P < 0.05) percentages of undifferentiated bfMSC were positive for NANOG (80.6%) and OCT4 (83.4%). DAZL- and NANOG-positive cells were 2.1% and 2.9%, and 95.9% and 97.8% at Days 0 and 21 of RA treatment, respectively. Data indicated that expression of germ cell biomarker DAZL in bfMSC is activated and increased after in vitro supplementation of RA and BMP4. Moreover, NANOG mRNA levels were regulated by RA treatment. Similar levels of SCP3 mRNA expression suggest that differentiated bfMSC were not induced into meiosis. Thus, exposure of bfMSC to RA or BMP4 under in vitro conditions might induce an early stage of premeiotic germinal differentiation.

scholarly journals The effect of a bone marrow-derived immunostimulatory preparation on the immunity of broiler chickens vaccinated against salmonellosis

2019 ◽  
Vol 64 (No. 7) ◽  
pp. 317-322
Author(s):  
N Mandro ◽  
YA Kopeikin ◽  
ZA Litvinova
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Bone Marrow Cells ◽  
Marrow Cell ◽  
Cell Protein ◽  
Phagocytic Index ◽  
Bovine Bone ◽  
Poultry Production ◽  
Protein Preparation ◽  
Marrow Cells

The use of bone marrow-derived immunostimulants is a promising direction in poultry production. The objective of this research was to study the effect of introducing a bone marrow cell protein formulation on the immunity of chickens vaccinated against salmonellosis. According to the principle of analogues, a control and two experimental groups of chickens were formed with 20 heads each (in total 60 individuals). To Group 1 birds, a protein preparation from bovine bone marrow cells was administered with feed by irrigation with 10% suspension in physiological saline at a rate of 0.2 ml per head once per day from the first day of life for three days. In Group 2, the drug was administered once, on day 1, at a rate of 0.2 ml per head. Control chickens were injected with saline in the same volumes. All chickens were vaccinated against salmonellosis. Blood for analysis of cellular, biochemical and humoral indicators was taken on days 7 and 14 of bird life. The use of the bone marrow cell-derived protein preparation resulted in higher values in the blood of chickens of Groups 1 and 2, respectively, by day 14 of age in comparison with controls as follows: erythrocytes (15.51% and 22.28%) and leukocytes (3.93% and 3.70%), T- and B- lymphocytes (67.5% and 69.16%; 23.24% and 23.75%), neutrophil phagocytic activity (10.14% and 25.36%) and phagocytic index (17.25% and 18.74%), bactericidal (13.32% and 20.25%) and lysozyme activity (23.88% and 24.41%), total protein (13.23% and 14.21%), immunoglobulins (19.59% and 20.76%), specific antibody titre (47.50% and 51.25%). Our study confirms the suitability of using bone marrow-derived protein preparations in poultry production. In practical terms, our study has particular importance for the development and implementation of preparations based on proteins of bone marrow cells.

Characterization of a novel decellularized bone marrow scaffold as an inductive environment for hematopoietic stem cells

2019 ◽  
Vol 7 (4) ◽  
pp. 1516-1528 ◽  
Author(s):  
Juares E. Romero Bianco ◽  
Renata Giardini Rosa ◽  
Ada Congrains-Castillo ◽  
Paulo P. Joazeiro ◽  
Stephen D. Waldman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Bovine Bone ◽  
Hematopoietic Stem ◽  
Bone Marrow Study ◽  
Increasing Demand

Due to the increasing demand for a bone marrow study model, we developed a natural scaffold from decellularized bovine bone marrow (DeBM).

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