A novel bi-enzymatic system for lactose conversion

2013 ◽  
Vol 48 (7) ◽  
pp. 1396-1403 ◽  
Author(s):  
Peter Chr. Lorenzen ◽  
Judith Breiter ◽  
Ingrid Clawin-Rädecker ◽  
Alexandra Dau
Keyword(s):  
Enzymatic System ◽  
Lactose Conversion

scholarly journals ENZYMATIC SYSTEM SYNTHESIZING SULFURIC ACID ESTERS OF PHENOLS

1955 ◽  
Vol 213 (1) ◽  
pp. 439-443 ◽  
Author(s):  
R.H. De Meio ◽  
Martha Wizerkaniuk ◽  
I. Schreibman
Keyword(s):  
Sulfuric Acid ◽  
Enzymatic System ◽  
Acid Esters

scholarly journals Effect of bioactive compounds released from Brassicaceae defatted seed meals on bacterial load in pig manure

2021 ◽  
Author(s):  
Luisa Ugolini ◽  
Donatella Scarafile ◽  
Roberto Matteo ◽  
Eleonora Pagnotta ◽  
Lorena Malaguti ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Bacterial Population ◽  
Bacterial Load ◽  
Bacterial Pathogen ◽  
Animal Manure ◽  
Pig Manure ◽  
Resistant Bacteria ◽  
Enzymatic System ◽  
Livestock Waste ◽  
Natural Substances

AbstractAnimal manure application to soils is considered to be one of the main cause of antibiotic and bacterial pathogen spread in the environment. Pig livestock, which is the source of one of the most used fertilizer for cultivated land, is also a hotspot for antibiotics and antibiotic-resistant bacteria. Besides harsh chemical and physical sanitization treatments for the abatement of antibiotics and bacterial load in livestock waste, more sustainable and environmentally friendly strategies need to be considered. In this context, the use of natural substances which are proved useful for pest and disease control is currently under exploration for their role in the reduction of bacterial pathogen population. Among these, plants and derived products from the Brassicaceae family, characterized by the presence of a defensive glucosinolate-myrosinase enzymatic system, have been successfully exploited for years in agriculture using the so-called biofumigation technique against crop diseases. Although the application of biofumigation to suppress a range of soil borne pests has been well documented, no studies have been examined to reduce bacterial population in animal waste. In the present study, the release and the antibacterial activity of bioactive compounds deriving from different Brassicaceae defatted seed meals against pathogens and bacterial population in pig manure is addressed. Rapistrum rugosum and Brassica nigra defatted seed meals were found to be the most active products against tested pathogens and able to significantly reduce the bacterial load in the manure.

Immobilized bi-enzymatic system for the determination of biogenic amines in solution

2021 ◽  
Vol 169 ◽  
pp. 107960
Author(s):  
Luca Lavagna ◽  
Maria Laura Tummino ◽  
Giuliana Magnacca ◽  
Ingrid Corazzari ◽  
Enzo Laurenti
Keyword(s):  
Biogenic Amines ◽  
Enzymatic System

The macrokinetics of an enzymatic system containing an enzyme that inactivates in the course of reaction

1983 ◽  
Vol 25 (11) ◽  
pp. 2519-2530 ◽  
Author(s):  
G. F. Sud'ina ◽  
G. M. Kobel'kov ◽  
S. D. Varfolomeev
Keyword(s):  
Enzymatic System

scholarly journals Synthesis of potentially-bioactive lactosyl-oligofructosides by a novel bi-enzymatic system using bacterial fructansucrases

2015 ◽  
Vol 78 ◽  
pp. 258-265 ◽  
Author(s):  
Marina Díez-Municio ◽  
Clara González-Santana ◽  
Blanca de las Rivas ◽  
M. Luisa Jimeno ◽  
Rosario Muñoz ◽  
...  
Keyword(s):  
Enzymatic System

scholarly journals Sesquiterpene Lactones from Artemisia absinthium. Biotransformation and Rearrangement of the Insect Antifeedant 3α-hydroxypelenolide

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 891
Author(s):  
Braulio M. Fraga ◽  
Carmen E. Díaz ◽  
María Bailén ◽  
Azucena González-Coloma
Keyword(s):  
Cytotoxic Activity ◽  
Leptinotarsa Decemlineata ◽  
Spodoptera Littoralis ◽  
Culture Medium ◽  
Insect Cells ◽  
Sesquiterpene Lactones ◽  
Enzymatic System ◽  
Artemisia Absinthium ◽  
Sf9 Insect Cells ◽  
New Compounds

Three new compounds, the sesquiterpenes absilactone and hansonlactone and the acetophenone derivative ajenjol, have been isolated from a cultivated variety of Artemisia absinthium. In addition, the major lactone isolated, 3α-hydroxypelenolide, was biotransformed by the fungus Mucor plumbeus affording the corresponding 1β, 10α-epoxide. A cadinane derivative was formed by an acid rearrangement produced in the culture medium, but not by the enzymatic system of the fungus. Furthermore, 3α-hydroxypelenolide showed strong antifeedant effects against Leptinotarsa decemlineata and cytotoxic activity to Sf9 insect cells, while the biotransformed compounds showed antifeedant postingestive effects against Spodoptera littoralis.

Influence of photodynamic processes induced by 2,2'-dipyridyl on the enzymatic system of chlorophyll biosynthesis

1998 ◽  
Vol 34 (4) ◽  
pp. 555-560 ◽  
Author(s):  
V. Toneva ◽  
N. Shalygo ◽  
E. Yaronskaya ◽  
N. Averina ◽  
I. Minkov
Keyword(s):  
Chlorophyll Biosynthesis ◽  
Enzymatic System

scholarly journals New Insights into the Potential of Endogenous Redox Systems in Wheat Bread Dough

Antioxidants ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 190 ◽  
Author(s):  
Nicolas Navrot ◽  
Rikke Buhl Holstborg ◽  
Per Hägglund ◽  
Inge Povlsen ◽  
Birte Svensson
Keyword(s):  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Thioredoxin Reductase ◽  
Heat Stability ◽  
Wheat Bread ◽  
Enzymatic System ◽  
Redox Systems ◽  
Bread Dough ◽  
Seed Formation ◽  
Protein Substrates ◽  
Thioredoxin System

Various redox compounds are known to influence the structure of the gluten network in bread dough, and hence its strength. The cereal thioredoxin system (NTS), composed of nicotinamide adenine dinucleotide phosphate (NADPH)-dependent thioredoxin reductase (NTR) and thioredoxin (Trx), is a major reducing enzymatic system that is involved in seed formation and germination. NTS is a particularly interesting tool for food processing due to its heat stability and its broad range of protein substrates. We show here that barley NTS is capable of remodeling the gluten network and weakening bread dough. Furthermore, functional wheat Trx that is present in the dough can be recruited by the addition of recombinant barley NTR, resulting in dough weakening. These results confirm the potential of NTS, especially NTR, as a useful tool in baking for weakening strong doughs, or in flat product baking.

scholarly journals Plant-Mediated Biotransformations of S(+)- and R(–)-Carvones

2018 ◽  
Vol 8 (12) ◽  
pp. 2605 ◽  
Author(s):  
Wanda Mączka ◽  
Daria Sołtysik ◽  
Katarzyna Wińska ◽  
Małgorzata Grabarczyk ◽  
Antoni Szumny
Keyword(s):  
Solanum Tuberosum L ◽  
Apium Graveolens ◽  
Petroselinum Crispum ◽  
Enzymatic System ◽  
Enantiomerically Pure ◽  
Daucus Carota L ◽  
Pyrus Communis L ◽  
Substrate Conversion ◽  
Diastereomeric Excess ◽  
Reduction Of Ketones

The enzymatic system of vegetables is well known as an efficient biocatalyst in the stereoselective reduction of ketones. Therefore, we decided to use the comminuted material of several plants including five vegetables (Apium graveolens L., Beta vulgaris L., Daucus carota L., Petroselinum crispum L., and Solanum tuberosum L.) and three fruits (Malus pumila L. “Golden” and “Kortland” as well as Pyrus communis L. “Konferencja”) to obtain enantiomerically pure carveol, which is commercially unavailable. Unexpectedly, all of the used biocatalysts not only reduced the carbonyl group of (4R)-(–)-carvone and (4S)-(+)-carvone, but also reduced the double bond in the cyclohexene ring. The results revealed that (4R)-(–)-carvone was transformed into (1R, 4R)- and (1S, 4R)-dihydrocarvones, and (1R,2R,4R)-dihydrocarveol. Although the enzymatic system of the potato transformed the substrate almost completely, the %de was not the highest. Potato yielded 92%; however, when carrot was used as the biocatalyst, it was possible to obtain 17% of (1R, 4R)-(+)-dihydrocarvone with 100% diastereomeric excess. In turn, the (4S)-(+)-carvone was transformed, using the biocatalysts, into (1R, 4S)- and (1S, 4S)-dihydrocarvones and dihydrocarveols. Complete substrate conversion was observed in biotransformation when potato was used. In the experiments using apple, (1R, 4S)-dihydrocarvone with 100% diastereomeric excess was obtained. Using NMR spectroscopy, we confirmed both diastereoisomers of 4(R)-1,2-dihydrocarveols, which were unseparated in the GC condition. Finally, we proved the high usefulness of vegetables for the biotransformation of both enantiomers of carvone as well as dihydrocarvone.

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