scholarly journals Improving lignocellulosic biofuel production by CRISPR/Cas9‐mediated lignin modification in barley

GCB Bioenergy ◽  
2021 ◽  
Author(s):  
Jae Hoon Lee ◽  
Hyo Jun Won ◽  
Phuong Hoang Nguyen Tran ◽  
Sun‐mi Lee ◽  
Ho‐Youn Kim ◽  
...  
Keyword(s):  
Biofuel Production ◽  
Lignin Modification ◽  
Lignocellulosic Biofuel

scholarly journals Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae

2021 ◽  
Vol 9 ◽  
Author(s):  
Minhye Shin ◽  
Heeyoung Park ◽  
Sooah Kim ◽  
Eun Joong Oh ◽  
Deokyeol Jeong ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Xylose Fermentation ◽  
Sugar Metabolism ◽  
Pentose Phosphate ◽  
Biofuel Production ◽  
Rna Seq ◽  
Loss Of Function ◽  
Lignocellulosic Biofuel ◽  
Pentose Sugar ◽  
Transcriptomic Changes

Being a microbial host for lignocellulosic biofuel production, Saccharomyces cerevisiae needs to be engineered to express a heterologous xylose pathway; however, it has been challenging to optimize the engineered strain for efficient and rapid fermentation of xylose. Deletion of PHO13 (Δpho13) has been reported to be a crucial genetic perturbation in improving xylose fermentation. A confirmed mechanism of the Δpho13 effect on xylose fermentation is that the Δpho13 transcriptionally activates the genes in the non-oxidative pentose phosphate pathway (PPP). In the current study, we found a couple of engineered strains, of which phenotypes were not affected by Δpho13 (Δpho13-negative), among many others we examined. Genome resequencing of the Δpho13-negative strains revealed that a loss-of-function mutation in GCR2 was responsible for the phenotype. Gcr2 is a global transcriptional factor involved in glucose metabolism. The results of RNA-seq confirmed that the deletion of GCR2 (Δgcr2) led to the upregulation of PPP genes as well as downregulation of glycolytic genes, and changes were more significant under xylose conditions than those under glucose conditions. Although there was no synergistic effect between Δpho13 and Δgcr2 in improving xylose fermentation, these results suggested that GCR2 is a novel knockout target in improving lignocellulosic ethanol production.

scholarly journals Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production

2020 ◽  
Vol 11 ◽  
Author(s):  
Mitra Mazarei ◽  
Holly L. Baxter ◽  
Avinash Srivastava ◽  
Guifen Li ◽  
Hongli Xie ◽  
...  
Keyword(s):  
Panicum Virgatum ◽  
Biofuel Production ◽  
Panicum Virgatum L ◽  
Lignocellulosic Biofuel

Toward high solids loading process for lignocellulosic biofuel production at a low cost

2017 ◽  
Vol 114 (5) ◽  
pp. 980-989 ◽  
Author(s):  
Mingjie Jin ◽  
Cory Sarks ◽  
Bryan D. Bals ◽  
Nick Posawatz ◽  
Christa Gunawan ◽  
...  
Keyword(s):  
Low Cost ◽  
Biofuel Production ◽  
High Solids ◽  
Loading Process ◽  
Solids Loading ◽  
High Solids Loading ◽  
Lignocellulosic Biofuel

Economics and Uncertainty of Lignocellulosic Biofuel Production from Energy Cane and Sweet Sorghum in South Texas

2014 ◽  
Vol 46 (4) ◽  
pp. 457-485 ◽  
Author(s):  
Juan J. Monge ◽  
Luis A. Ribera ◽  
John L. Jifon ◽  
Jorge A. da Silva ◽  
James W. Richardson
Keyword(s):  
Sweet Sorghum ◽  
Net Present Value ◽  
Economic Feasibility ◽  
South Texas ◽  
Government Support ◽  
Biofuel Production ◽  
Energy Cane ◽  
Cellulosic Biofuel ◽  
Lignocellulosic Biofuel ◽  
Yield Information

Government support uncertainty, scarce yield information, and the inherent risk in bioeconomic phenomena are some of the deterrents faced by investors in the nascent cellulosic biofuel industry. A financial probabilistic model was developed to contrast the economic feasibility of producing cellulosic biofuels from energy cane and sweet sorghum using three technologies: hydrolysis, pyrolysis, and gasification. Hydrolysis and pyrolysis proved feasible (showed possibilities of a positive net present value) without government support and conditioned to stochastic feedstock yields and biofuel prices. Gasification was feasible with government support. Improved feedstock and biofuel productivity would considerably raise the feasibility probabilities for hydrolysis and pyrolysis without government support.

scholarly journals The Anaerobic Fungi: Challenges and Opportunities for Industrial Lignocellulosic Biofuel Production

2021 ◽  
Vol 9 (4) ◽  
pp. 694
Author(s):  
Luke M. G. Saye ◽  
Tejas A. Navaratna ◽  
James P. J. Chong ◽  
Michelle A. O’Malley ◽  
Michael K. Theodorou ◽  
...  
Keyword(s):  
Natural Habitat ◽  
Biofuel Production ◽  
Anaerobic Fungi ◽  
Industrial Systems ◽  
Microbial Electrolysis Cells ◽  
Lignocellulosic Biofuel ◽  
Electrolysis Cells ◽  
Biological Conversion ◽  
Challenges And Opportunities

Lignocellulose is a promising feedstock for biofuel production as a renewable, carbohydrate-rich and globally abundant source of biomass. However, challenges faced include environmental and/or financial costs associated with typical lignocellulose pretreatments needed to overcome the natural recalcitrance of the material before conversion to biofuel. Anaerobic fungi are a group of underexplored microorganisms belonging to the early diverging phylum Neocallimastigomycota and are native to the intricately evolved digestive system of mammalian herbivores. Anaerobic fungi have promising potential for application in biofuel production processes due to the combination of their highly effective ability to hydrolyse lignocellulose and capability to convert this substrate to H2 and ethanol. Furthermore, they can produce volatile fatty acid precursors for subsequent biological conversion to H2 or CH4 by other microorganisms. The complex biological characteristics of their natural habitat are described, and these features are contextualised towards the development of suitable industrial systems for in vitro growth. Moreover, progress towards achieving that goal is reviewed in terms of process and genetic engineering. In addition, emerging opportunities are presented for the use of anaerobic fungi for lignocellulose pretreatment; dark fermentation; bioethanol production; and the potential for integration with methanogenesis, microbial electrolysis cells and photofermentation.

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