Toward high solids loading process for lignocellulosic biofuel production at a low cost

2017 ◽  
Vol 114 (5) ◽  
pp. 980-989 ◽  
Author(s):  
Mingjie Jin ◽  
Cory Sarks ◽  
Bryan D. Bals ◽  
Nick Posawatz ◽  
Christa Gunawan ◽  
...  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Meenaa Chandrasekar ◽  
Leela Joshi ◽  
Karleigh Krieg ◽  
Sarvada Chipkar ◽  
Emily Burke ◽  
...  

Abstract Background Environmental factors, such as weather extremes, have the potential to cause adverse effects on plant biomass quality and quantity. Beyond adversely affecting feedstock yield and composition, which have been extensively studied, environmental factors can have detrimental effects on saccharification and fermentation processes in biofuel production. Only a few studies have evaluated the effect of these factors on biomass deconstruction into biofuel and resulting fuel yields. This field-to-fuel evaluation of various feedstocks requires rigorous coordination of pretreatment, enzymatic hydrolysis, and fermentation experiments. A large number of biomass samples, often in limited quantity, are needed to thoroughly understand the effect of environmental conditions on biofuel production. This requires greater processing and analytical throughput of industrially relevant, high solids loading hydrolysates for fermentation, and led to the need for a laboratory-scale high solids experimentation platform. Results A field-to-fuel platform was developed to provide sufficient volumes of high solids loading enzymatic hydrolysate for fermentation. AFEX pretreatment was conducted in custom pretreatment reactors, followed by high solids enzymatic hydrolysis. To accommodate enzymatic hydrolysis of multiple samples, roller bottles were used to overcome the bottlenecks of mixing and reduced sugar yields at high solids loading, while allowing greater sample throughput than possible in bioreactors. The roller bottle method provided 42–47% greater liquefaction compared to the batch shake flask method for the same solids loading. In fermentation experiments, hydrolysates from roller bottles were fermented more rapidly, with greater xylose consumption, but lower final ethanol yields and CO2 production than hydrolysates generated with shake flasks. The entire platform was tested and was able to replicate patterns of fermentation inhibition previously observed for experiments conducted in larger-scale reactors and bioreactors, showing divergent fermentation patterns for drought and normal year switchgrass hydrolysates. Conclusion A pipeline of small-scale AFEX pretreatment and roller bottle enzymatic hydrolysis was able to provide adequate quantities of hydrolysate for respirometer fermentation experiments and was able to overcome hydrolysis bottlenecks at high solids loading by obtaining greater liquefaction compared to batch shake flask hydrolysis. Thus, the roller bottle method can be effectively utilized to compare divergent feedstocks and diverse process conditions.


2021 ◽  
Author(s):  
Jose A. Perez-Pimienta ◽  
Gabriella Papa ◽  
Jian Sun ◽  
Vitalie Stavila ◽  
Arturo Sanchez ◽  
...  

Agave bagasse (AG) is used as potential bioenergy feedstock due to its high biomass productivity, even in semiarid lands. In particular, Ionic liquid (IL) pretreatment using aprotic ILs (AILs) has...


2012 ◽  
Vol 468-471 ◽  
pp. 2078-2081
Author(s):  
Qing Ye ◽  
Jun Jie Hao ◽  
Zhi Meng Guo ◽  
Min Chao Ru

Ti-6Al-4V alloy was prepared by gelcasting. Plasma rotating electrode and hydrogenation-dehydrogenation Ti-6Al-4V powders were mixed used to meet both high performance and low cost objectives. The morphological properties were investigated by SEM. The results showed that the gelcasting green body with mixed powders has advantages of high solids loading and excellent sinterability. After sintered at 1400°C for 2h, the relative density reached 98%. The bending strength and hardness were 313MPa and 49.8(HRC).


2016 ◽  
Vol 111 ◽  
pp. 387-402 ◽  
Author(s):  
Elin Svensson ◽  
Valeria Lundberg ◽  
Mikael Jansson ◽  
Charilaos Xiros ◽  
Thore Berntsson

2001 ◽  
Vol 206-213 ◽  
pp. 75-78
Author(s):  
O. Lyckfeldt ◽  
Lisa Palmqvist ◽  
Frederic Poeydemenge

2018 ◽  
Vol 250 ◽  
pp. 273-280 ◽  
Author(s):  
Bárbara Ribeiro Alves Alencar ◽  
Emmanuel Damilano Dutra ◽  
Everardo Valadares de Sá Barretto Sampaio ◽  
Rômulo Simões Cezar Menezes ◽  
Marcos Antônio Morais

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