In vivostudy for the discrimination of cancerous and normal skin using fibre probe-based Raman spectroscopy

2015 ◽  
Vol 24 (10) ◽  
pp. 767-772 ◽  
Author(s):  
Johannes Schleusener ◽  
Patrycja Gluszczynska ◽  
Carina Reble ◽  
Ingo Gersonde ◽  
Jürgen Helfmann ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Normal Skin ◽  
Fibre Probe

Raman spectroscopy for the discrimination of cancerous and normal skin

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Johannes Schleusener ◽  
Carina Reble ◽  
Martina C. Meinke ◽  
Jürgen Helfmann
Keyword(s):  
Raman Spectroscopy ◽  
Normal Skin ◽  
Skin Cancers

Abstract:Various studies have shown promising results in using Raman spectroscopy (RS) for the detection of skin cancers.

scholarly journals FT-Raman spectroscopy for the differentiation between cutaneous melanoma and pigmented nevus

2010 ◽  
Vol 25 (4) ◽  
pp. 351-356 ◽  
Author(s):  
Sidney Bandeira Cartaxo ◽  
Ivan Dunshee de Abranches Oliveira Santos ◽  
Renata Bitar ◽  
Andréa Fernandes Oliveira ◽  
Lydia Masako Ferreira ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cutaneous Melanoma ◽  
Significant Variation ◽  
Ex Vivo ◽  
Normal Skin ◽  
Univariate Analysis ◽  
Ft Raman Spectroscopy ◽  
Pigmented Nevus ◽  
Pigmented Nevi ◽  
Ft Raman

Cutaneous melanoma is the most aggressive type of skin cancer and Ft-Raman spectroscopy has been studied as a potential method that could be a real alternative for early diagnosis of neoplasms. PURPOSE: To qualify the spectral FT-Raman data, in order to differentiate cutaneous melanoma and pigmented nevus. METHODS: For this study, 10 samples of cutaneous melanoma, 9 samples of pigmented nevi, and 10 samples of normal skin were obtained by incisional biopsies performed during plastic surgeries ex vivo, immediately after removing the surgical sample. RESULTS: The FT-Raman spectra of each group presented a high correlation between the elements of the same group, thus favoring the elaboration of spectral averages. When analyzing the spectral standard of each group, the normal skin standard did not show a significant variation between the spectra; the standard of the pigmented nevi group showed significant variation, and the cutaneous melanoma group also showed variation. Through univariate analysis, specific bands were detected for each vibrational mode identified. The discriminatory analysis of the data showed a 75.3% efficiency of the differentiation between the three groups studied. CONCLUSION: The vibrational modes Polysaccharides, Tyrosine and Amide-I differentiated the melanoma from the pigmented nevus.

scholarly journals Sub-millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

2021 ◽  
Author(s):  
Stephanos Yerolatsitis ◽  
András Kufcsák ◽  
Katjana Ehrlich ◽  
Harry Wood ◽  
Susan Fernandes ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fibre Probe

scholarly journals Micro-Lensed Negative-Curvature Fibre Probe for Raman Spectroscopy

Sensors ◽  
2021 ◽  
Vol 21 (24) ◽  
pp. 8434
Author(s):  
Karolina Milenko ◽  
Stephanos Yerolatsitis ◽  
Astrid Aksnes ◽  
Dag Roar Hjelme ◽  
James M. Stone
Keyword(s):  
Raman Spectroscopy ◽  
Aqueous Solutions ◽  
Negative Curvature ◽  
Collection Efficiency ◽  
Fibre Probe

We developed a novel miniature micro-lensed fibre probe for Raman spectroscopy. The fibre probe consists of a single negative-curvature fibre (NCF) and a spliced, cleaved, micro-lensed fibre cap. Using a single NCF, we minimized the Raman background generated from the silica and maintained the diameter of the probe at less than 0.5 mm. In addition, the cap provided fibre closure by blocking the sample from entering the hollow parts of the fibre, enabling the use of the probe in in vivo applications. Moreover, the micro-lensed cap offered an improved collection efficiency (1.5-times increase) compared to a cleaved end-cap. The sensing capabilities of the micro-lensed probe were demonstrated by measuring different concentrations of glucose in aqueous solutions.

scholarly journals Sub millimetre flexible fibre probe for background and fluorescence free Raman spectroscopy

2021 ◽  
Author(s):  
Stephanos Yerolatsitis ◽  
András Kufcsák ◽  
Katjana Ehrlich ◽  
Harry A. C. Wood ◽  
Susan Fernandes ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fibre Probe

Raman spectroscopy differentiates squamous cell carcinoma (SCC) from normal skin following treatment with a high-powered CO2 laser

2014 ◽  
Vol 46 (10) ◽  
pp. 757-772 ◽  
Author(s):  
Sara A. Fox ◽  
Ashley A. Shanblatt ◽  
Hugh Beckman ◽  
John Strasswimmer ◽  
Andrew C. Terentis
Keyword(s):  
Raman Spectroscopy ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Co2 Laser ◽  
Squamous Cell ◽  
Normal Skin

scholarly journals Differential diagnosis in primary and metastatic cutaneous melanoma by FT-Raman spectroscopy

2010 ◽  
Vol 25 (5) ◽  
pp. 434-439 ◽  
Author(s):  
Andrea Fernandes de Oliveira ◽  
Ivan Dunshee de Abranches Oliveira Santos ◽  
Sidney Bandeira Cartaxo ◽  
Renata Andrade Bitar ◽  
Mílvia Maria Simões e Silva Enokihara ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Differential Diagnosis ◽  
Cutaneous Melanoma ◽  
Normal Skin ◽  
Normal Human Skin ◽  
Ft Raman Spectroscopy ◽  
Normal Human ◽  
Raman Spectral Data ◽  
Raman Spectral ◽  
Ft Raman

PURPOSE: To qualify the FT-Raman spectral data of primary and metastatic cutaneous melanoma in order to obtain a differential diagnosis. METHODS: Ten normal human skin samples without any clinical or histopathological alterations, ten cutaneous melanoma fragments, and nine lymph node metastasis samples were used; 105, 140 and 126 spectra were obtained respectively. Each sample was divided into 2 or 3 fragments of approximately 2 mm³ and positioned in the Raman spectrometer sample holder in order to obtain the spectra; a monochrome laser light Nd:YAG at 1064 nm was used to excite the inelastic effect. RESULTS: To differentiate the three histopathological groups according to their characteristics extracted from the spectra, data discriminative analysis was undertaken. Phenylalanine, DNA, and Amide-I spectral variables stood out in the differentiation of the three groups. The percentages of correctly classified groups based on Phenylalanine, DNA, and Amide-I spectral features was 93.1%. CONCLUSION: FT-Raman spectroscopy is capable of differentiating melanoma from its metastasis, as well as from normal skin.

Discrimination of non-melanoma skin cancer and keratosis from normal skin tissue in vivo and ex vivo by Raman spectroscopy

2019 ◽  
Vol 100 ◽  
pp. 131-141 ◽  
Author(s):  
Ana Mara Ferreira Lima ◽  
Camila Ribeiro Daniel ◽  
Ricardo Scarparo Navarro ◽  
Benito Bodanese ◽  
Carlos Augusto Pasqualucci ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Skin Cancer ◽  
Ex Vivo ◽  
Normal Skin ◽  
Skin Tissue ◽  
Non Melanoma Skin Cancer ◽  
Melanoma Skin

scholarly journals Discrimination of Basal Cell Carcinoma and Melanoma from Normal Skin Biopsies in Vitro Through Raman Spectroscopy and Principal Component Analysis

2012 ◽  
Vol 30 (7) ◽  
pp. 381-387 ◽  
Author(s):  
Benito Bodanese ◽  
Fabrício Luiz Silveira ◽  
Renato Amaro Zângaro ◽  
Marcos Tadeu T. Pacheco ◽  
Carlos Augusto Pasqualucci ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Normal Skin ◽  
Principal Component ◽  
Component Analysis ◽  
Skin Biopsies

Fibroblasts During Hypertrophic Scar Formation and Resolution

1973 ◽  
Vol 31 ◽  
pp. 428-429
Author(s):  
C. W. Kischer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Fine Structure ◽  
Metabolic Activity ◽  
Hypertrophic Scar ◽  
Normal Skin ◽  
Ground Substance ◽  
Hypertrophic Scars ◽  
Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

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