scholarly journals Discrimination of Basal Cell Carcinoma and Melanoma from Normal Skin Biopsies in Vitro Through Raman Spectroscopy and Principal Component Analysis

2012 ◽  
Vol 30 (7) ◽  
pp. 381-387 ◽  
Author(s):  
Benito Bodanese ◽  
Fabrício Luiz Silveira ◽  
Renato Amaro Zângaro ◽  
Marcos Tadeu T. Pacheco ◽  
Carlos Augusto Pasqualucci ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Normal Skin ◽  
Principal Component ◽  
Component Analysis ◽  
Skin Biopsies

103 RAMAN MICROSPECTROSCOPY AS A TOOL TO DETECT MOLECULAR MODIFICATIONS INDUCED BY AGING-RELATED OXIDATIVE STRESS IN MOUSE OOCYTES

2013 ◽  
Vol 25 (1) ◽  
pp. 199
Author(s):  
O. Murrone ◽  
M. Piccinini ◽  
C. Tatone ◽  
G. Di Emidio ◽  
S. Ledda ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Principal Component ◽  
Component Analysis ◽  
Oocyte Quality ◽  
Markers Of Oxidative Stress ◽  
Group A ◽  
Old Mice

The conditions of oxidative stress that can be generated during physiological events, such as post-ovulatory aging and reproductive aging, as well as by the PMA procedures, can seriously degrade the oocyte developmental competence. The ability to identify predictive markers of oxidative stress using noninvasive techniques may provide a useful diagnostic tool for the assessment of oocyte quality. The aim of the present work is to evaluate the potential of Raman spectroscopy (RMN) as a tool to detect molecular modifications induced by aging-related oxidative stress in mouse oocytes. The research was carried out using CD-1 mice; at the age of 4 to 8 weeks (young mice) and 48 to52 weeks (old mice), females were superovulated and oocytes at metaphase II stage were recovered from oviducts. The MII oocytes from young animals were divided into 3 experimental groups: (A) young oocytes, processed immediately after collection; (B) in vitro aged oocytes, cultured in vitro for 10 h before processing; (C) oxidative-stressed oocytes, exposed to 10 mM hydrogen peroxide for 2 min before processing (oocytes with a fully oxidized status). Oocytes from reproductively old mice were referred to as old oocytes (D). After fixation in 3.7% paraformaldehyde, oocytes (n = 10 for each group) were immersed in a 50-µL drop of PBS on quartz windows and analyzed using a Bruker Senterra confocal Raman microscope. Measurements were performed by recording 3 line scans across the oocyte with 5-µm step size, totalling 32 point spectra for each oocyte. The spectra were statistically analyzed using principal component analysis. Principal component analysis showed a clear discrimination between the spectra of young oocytes (A), in vitro aged oocytes (B), oxidative-stressed oocytes (C), and old oocytes (D). Compared with the control group (A), B, C, and D groups revealed significant differences in the bands attributable to lipid components; specifically, a reduction in the intensity of the peaks at 1653 and 1602 cm–1 (stretching of the C = C bond) and of the peaks at 1485, 1462, 1437, 1396 cm–1 (CH3-CH2 vibration) was recorded. With regard to the protein component, spectra of B, C, and D groups showed modifications in the intensities of peaks 1297 and 850 cm–1, which refer respectively to amide III and to CNC symmetric stretching compared with group A. Principal component analysis also revealed an overlap between the spectra of in vitro aged oocytes, old oocytes, and oxidative-stressed oocytes, suggesting that the molecular damage caused by ageing has similar characteristics to chemically induced oxidative damage. In conclusion, the results of our study show that Raman spectroscopy is a valuable tool for the identification of molecular biochemical markers of oxidative stress. This technique could represent a highly informative method of investigation to evaluate the oocyte quality in response to various stress conditions (in vitro maturation, aging, cryopreservation, and so on) that may negatively affect its potential development.

Formulation, Characterization and In vitro Cytotoxicity of 5-Fluorouracil Loaded Polymeric Electrospun Nanofibers for the Treatment of Skin Cancer

2019 ◽  
Vol 13 (2) ◽  
pp. 114-128 ◽  
Author(s):  
Gayatri Patel ◽  
Bindu K.N. Yadav
Keyword(s):  
Skin Cancer ◽  
Basal Cell Carcinoma ◽  
Cell Viability ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Fiber Diameter ◽  
Electrospun Nanofibers ◽  
In Vitro Cytotoxicity ◽  
Fractional Factorial

Background: The purpose of this study was to formulate, characterize and conduct in vitro cytotoxicity of 5-fluorouracil loaded polymeric electrospun nanofibers for the treatment of skin cancer. The patents on electrospun nanofibers (US9393216B2), (US14146252), (WO2015003155A1) etc. helped in the selection of polymers and method for the preparation of nanofibers. Methods: In the present study, the fabrication of nanofibers was done using a blend of chitosan with polyvinyl alcohol and processed using the electrospinning technique. 5-fluorouracil with known chemotherapeutic potential in the treatment of skin cancer was used as a drug carrier. 24-1 fractional factorial screening design was employed to study the effect of independent variables like the concentration of the polymeric solution, applied voltage (kV), distance (cm), flow rate (ml / hr) on dependent variables like % entrapment efficiency and fiber diameter. Results: Scanning electron microscopy was used to characterize fiber diameter and morphology. Results showed that the fiber diameter of all batches was found in the range of 100-200 nm. The optimized batch results showed the fiber diameter of 162.7 nm with uniform fibers. The tensile strength obtained was 190±37 Mpa. Further in vitro and ex vivo drug release profile suggested a controlled release mechanism for an extended period of 24 hr. The 5-fluorouracil loaded electrospun nanofibers were found to decrease cell viability up to ≥50% over 24 hr, with the number of cells dropping by ~ 10% over 48 hr. As the cell viability was affected by the release of 5-fluorouracil, we believe that electrospun nanofibers are a promising drug delivery system for the treatment of Basal Cell Carcinoma (BCC) skin cancer. Conclusion: These results demonstrate the possibility of delivering 5-Fluorouracil loaded electrospun nanofiber to skin with enhanced encapsulation efficiency indicating the effectiveness of the formulation for the treatment of basal cell carcinoma type of skin cancer.

scholarly journals Formulations Based on Drug Loaded Aptamer-Conjugated Liposomes as a Viable Strategy for the Topical Treatment of Basal Cell Carcinoma—In Vitro Tests

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 866
Author(s):  
Anca N. Cadinoiu ◽  
Delia M. Rata ◽  
Leonard I. Atanase ◽  
Cosmin T. Mihai ◽  
Simona E. Bacaita ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Topical Treatment ◽  
Skin Irritation ◽  
In Vitro Tests ◽  
Liposomal Drug ◽  
Liposomal Formulations ◽  
As1411 Aptamer

Topical liposomal drug formulations containing AS1411-aptamer conjugated liposomes were designed to deliver in a sustained way the 5-fluorouracil to the tumor site but also to increase the compliance of patients with basal cell carcinoma. The 5-fluorouracil penetrability efficiency through the Strat-M membrane and the skin irritation potential of the obtained topical liposomal formulations were evaluated in vitro and the Korsmeyer Peppas equation was considered as the most appropriate to model the drug release. Additionally, the efficiency of cytostatic activity for targeted antitumor therapy and the hemolytic capacity were performed in vitro. The obtained results showed that the optimal liposomal formulation is a crosslinked gel based on sodium alginate and hyaluronic acid containing AS1411-aptamer conjugated liposomes loaded with 5-fluorouracil, which appeared to have favorable biosafety effects and may be used as a new therapeutic approach for the topical treatment of basal cell carcinoma.

scholarly journals UV-Radiation-Specific p53 Mutation Frequency in Normal Skin as a Predictor of Risk of Basal Cell Carcinoma

1998 ◽  
Vol 90 (7) ◽  
pp. 523-531 ◽  
Author(s):  
Allal Ouhtit ◽  
Hisayoshi Nakazawa ◽  
Hiroshi Yamasaki ◽  
Bruce K. Armstrong ◽  
Anne Kricker ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Uv Radiation ◽  
Basal Cell ◽  
Mutation Frequency ◽  
Normal Skin ◽  
P53 Mutation

Investigation of sport supplements quality by Raman spectroscopy and principal component analysis

2016 ◽  
Vol 87 ◽  
pp. 1-7 ◽  
Author(s):  
Mariana R. Almeida ◽  
Letícia P. de Souza ◽  
Rodrigo S. Cesar ◽  
Rafael A. Sousa ◽  
Celly M.S. Izumi
Keyword(s):  
Principal Component Analysis ◽  
Raman Spectroscopy ◽  
Principal Component ◽  
Component Analysis ◽  
Sport Supplements
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