Shiga toxin 2eB‐transgenic lettuce vaccine is effective in protecting weaned piglets from edema disease caused by Shiga toxin‐producing Escherichia coli infection

2019 ◽  
Vol 90 (11) ◽  
pp. 1460-1467 ◽  
Author(s):  
Takashi Hamabata ◽  
Toshio Sato ◽  
Eiji Takita ◽  
Takeshi Matsui ◽  
Taishi Imaoka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Weaned Piglets ◽  
Transgenic Lettuce ◽  
Edema Disease ◽  
Escherichia Coli Infection

scholarly journals Structural Insights into Escherichia coli Shiga Toxin (Stx) Glycosphingolipid Receptors of Porcine Renal Epithelial Cells and Inhibition of Stx-Mediated Cellular Injury Using Neoglycolipid-Spiked Glycovesicles

2019 ◽  
Vol 7 (11) ◽  
pp. 582 ◽  
Author(s):  
Johanna Detzner ◽  
Caroline Gloerfeld ◽  
Gottfried Pohlentz ◽  
Nadine Legros ◽  
Hans-Ulrich Humpf ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Shiga Toxin ◽  
Spectrometric Analysis ◽  
Porcine Kidney ◽  
Renal Epithelial Cells ◽  
Edema Disease ◽  
Kidney Epithelial Cell ◽  
Inhibitory Potential

Shiga toxin (Stx) producing Escherichia coli (STEC) cause the edema disease in pigs by releasing the swine-pathogenic Stx2e subtype as the key virulence factor. Stx2e targets endothelial cells of animal organs including the kidney harboring the Stx receptor glycosphingolipids (GSLs) globotriaosylceramide (Gb3Cer, Galα1-4Galβ1-4Glcβ1-1Cer) and globotetraosylceramide (Gb4Cer, GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1Cer). Since the involvement of renal epithelial cells in the edema disease is unknown, in this study, we analyzed the porcine kidney epithelial cell lines, LLC-PK1 and PK-15, regarding the presence of Stx-binding GSLs, their sensitivity towards Stx2e, and the inhibitory potential of Gb3- and Gb4-neoglycolipids, carrying phosphatidylethanolamine (PE) as the lipid anchor, towards Stx2e. Immunochemical and mass spectrometric analysis revealed various Gb3Cer and Gb4Cer lipoforms as the dominant Stx-binding GSLs in both LLC-PK1 and PK-15 cells. A dihexosylceramide with proposed Galα1-4Gal-sequence (Gal2Cer) was detected in PK-15 cells, whereas LLC-PK1 cells lacked this compound. Both cell lines were susceptible towards Stx2e with LLC-PK1 representing an extremely Stx2e-sensitive cell line. Gb3-PE and Gb4-PE applied as glycovesicles significantly reduced the cytotoxic activity of Stx2e towards LLC-PK1 cells, whereas only Gb4-PE exhibited some protection against Stx2e for PK-15 cells. This is the first report identifying Stx2e receptors of porcine kidney epithelial cells and providing first data on their Stx2e-mediated damage suggesting possible involvement in the edema disease.

scholarly journals Haemolytic uraemic syndrome and Shiga toxin-producing Escherichia coli infection in children in France

2000 ◽  
Vol 124 (2) ◽  
pp. 215-220 ◽  
Author(s):  
B. DECLUDT ◽  
P. BOUVET ◽  
P. MARIANI-KURKDJIAN ◽  
F. GRIMONT ◽  
P. A. D. GRIMONT ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Prospective Study ◽  
Shiga Toxin ◽  
Haemolytic Uraemic Syndrome ◽  
Serum Samples ◽  
E Coli ◽  
Escherichia Coli Infection ◽  
Stool Samples ◽  
Polymerase Chain

We conducted a study to determine the incidence of haemolytic uraemic syndrome (HUS) in children in France and to assess the role of Shiga-toxin-producing Escherichia coli (STEC) infection in the aetiology of HUS. In collaboration with the Société de Néphrologie Pédiatrique we undertook a retrospective review of all cases of HUS hospitalized from January 1993 to March 1995 and a 1-year prospective study (April 1995–March 1996) of epidemiological and microbiological features of cases of HUS. The polymerase chain reaction (PCR) procedure was used to detect stx, eae, e-hlyA genes directly from case stool samples. Serum samples from cases were examined for antibodies to lipopolysaccharide (LPS) of 26 major STEC serogroups. Two hundred and eighty-six cases were reported. The average incidence per year was 0·7/105 children < 15 years and 1·8/105 children < 5 years. During the prospective study, 122/130 cases were examined for evidence of STEC infection using PCR and/or serological assays and 105 (86%) had evidence of STEC infection. Serum antibodies to E. coli O157 LPS were detected in 79 (67%) cases tested. In conclusion, this study showed that STEC infection is an important cause of HUS in children in France, with a high proportion related to the O157 serogroup.

Production of double repeated B subunit of Shiga toxin 2e at high levels in transgenic lettuce plants as vaccine material for porcine edema disease

2010 ◽  
Vol 20 (4) ◽  
pp. 735-748 ◽  
Author(s):  
Takeshi Matsui ◽  
Eiji Takita ◽  
Toshio Sato ◽  
Michie Aizawa ◽  
Misa Ki ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Transgenic Lettuce ◽  
Edema Disease ◽  
B Subunit

scholarly journals Shiga Toxin-producing Escherichia coli Infection in Germany--Different Risk Factors for Different Age Groups

2006 ◽  
Vol 165 (4) ◽  
pp. 425-434 ◽  
Author(s):  
D. Werber ◽  
S. C. Behnke ◽  
A. Fruth ◽  
R. Merle ◽  
S. Menzler ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Age Groups ◽  
Escherichia Coli Infection

scholarly journals Prevalence of a Gene Encoding Adhesin Involved in Diffuse Adherence among Escherichia Coli Isolates in Pigs with Postweaning Diarrhea or Edema Disease

2003 ◽  
Vol 15 (4) ◽  
pp. 378-381 ◽  
Author(s):  
Seung-Kwon Ha ◽  
Changsun Choi ◽  
Chanhee Chae
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Coli Strain ◽  
Isolation Rate ◽  
Gene Encoding ◽  
Edema Disease ◽  
E Coli ◽  
Heat Stable ◽  
Fimbrial Adhesins ◽  
Postweaning Diarrhea

A total of 604 Escherichia coli strains isolated from weaned pigs with diarrhea or edema disease on 653 swine farms were screened for the presence of the adhesin involved in diffuse adherence (AIDA) gene by polymerase chain reaction (PCR). Escherichia coli isolates that carried AIDA genes were also tested by PCR for the detection of 5 fimbriae (F4, F5, F6, F18, and F41), 3 heat-stable (STa, STb, and EAST1) and 1 heat-labile (LT) enterotoxin, and Shiga toxin 2e (Stx2e) genes. Forty-five (7.5%) of the 604 E. coli isolates carried the gene for AIDA. Of these 45 isolates, 5 (11.1%) carried EAST1 genes only, 1 (2.2%) carried genes for at least one of the fimbrial adhesins, 12 (26.7%) carried genes for at least one of the toxins, and 27 (60%) carried genes for at least one of the fimbrial adhesins and toxins. Fifty-one percent of strains that carried AIDA genes carried Stx2e genes, and 40% of strains that carried AIDA genes carried F18ab. The isolation rate of enterotoxigenic E. coli strain carrying genes for AIDA was 87%, and the isolation rate of Shiga toxin-producing E. coli strain carrying genes for AIDA was 49%. AIDA may represent an important virulence determinant in pigs with postweaning diarrhea or edema disease.

Escherichia coli strains from edema disease: O serogroups, and genes for Shiga toxin, enterotoxins, and F18 fimbriae

2001 ◽  
Vol 80 (3) ◽  
pp. 227-233 ◽  
Author(s):  
Alex Souza da Silva ◽  
Geórgio Freesz Valadares ◽  
Mario Paulo Amante Penatti ◽  
Benito Guimarães Brito ◽  
Domingos da Silva Leite
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Edema Disease

scholarly journals Neuropsychological Outcome after Complicated Shiga Toxin–Producing Escherichia coli Infection

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e103029 ◽  
Author(s):  
Olga Simova ◽  
Gabriele Weineck ◽  
Thorsten Schuetze ◽  
Karl Wegscheider ◽  
Ulf Panzer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Neuropsychological Outcome ◽  
Escherichia Coli Infection
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