Carotenoid production by the marine microalgae Nannochloropsis oculata in different low-cost culture media

2018 ◽  
Vol 49 (7) ◽  
pp. 2527-2535 ◽  
Author(s):  
Wladimir Angelino Faé Neto ◽  
Carlos Rafael Borges Mendes ◽  
Paulo Cesar Abreu
Keyword(s):  
Culture Media ◽  
Low Cost ◽  
Nannochloropsis Oculata ◽  
Marine Microalgae ◽  
Carotenoid Production

scholarly journals Improvement of a Specific Culture Medium Based on Industrial Glucose for Carotenoid Production by Xanthophyllomyces dendrorhous

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 429
Author(s):  
Alejandro Torres-Haro ◽  
Anne Gschaedler ◽  
Juan C. Mateos-Díaz ◽  
Enrique J. Herrera-López ◽  
Rosa M. Camacho-Ruíz ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
Carotenoid Biosynthesis ◽  
Pilot Scale ◽  
P Value ◽  
Xanthophyllomyces Dendrorhous ◽  
Carotenoid Production ◽  
Yeast Peptone Dextrose ◽  
Specific Culture

In this study, a low-cost chemically defined (CD) culture medium was proposed and evaluated with the aim of replacing culture media such as yeast mold (YM) and yeast peptone dextrose (YPD), commonly used for growth and carotenoid production by Xanthophyllomyces dendrorhous. Initially, the CD culture medium was compared to the YM and YPD. The growth in optical density (OD) and carotenoid production (mg/L) of the cultures reached 4.88, 6.76, 5.79, and 0.67, 0.92, and 0.69, respectively. The CD culture served as the basis of an improved specific culture medium containing industrial glucose. Additionally, in this new formulation, vitamins, glutamate, and other compounds were evaluated. Industrial glucose more than doubled carotenoid production; however, the addition of vitamins was not essential for X. dendrorhous cultivation. Moreover, glutamate and Na2HPO4 proved to be highly significant factors (p-value < 0.05), increasing carotenoid biosynthesis from 0.67 to 1.33 mg/L. The specific culture was successfully used in a bioreactor at 2 L and 110 L pilot-scale levels, increasing carotenoid production up to 2 mg/L. It was demonstrated that the CD-specific culture medium is an efficient alternative to conventional culture media to carry out carotenoid production at the laboratory and pilot levels, with promising potential for industrial scaling.

A photobioreactor using Nannochloropsis oculata marine microalgae for removal of polycyclic aromatic hydrocarbons and sorption of metals in produced water

Chemosphere ◽  
2021 ◽  
pp. 130775
Author(s):  
Isadora Machado Marques ◽  
Adna Caroline Vale Oliveira ◽  
Olivia Maria Cordeiro de Oliveira ◽  
Emerson Andrade Sales ◽  
Ícaro Thiago Andrade Moreira
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Produced Water ◽  
Nannochloropsis Oculata ◽  
Marine Microalgae ◽  
Polycyclic Aromatic

scholarly journals Development of single-cell-level microfluidic technology for long-term growth visualization of living cultures of Mycobacterium smegmatis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Han Wang ◽  
Gloria M. Conover ◽  
Song-I Han ◽  
James C. Sacchettini ◽  
Arum Han
Keyword(s):  
Drug Treatment ◽  
Single Cell ◽  
Mycobacterium Smegmatis ◽  
Culture Media ◽  
Low Cost ◽  
Bacterial Pathogen ◽  
Growth Dynamics ◽  
Time Lapse ◽  
Cell Phenotype

AbstractAnalysis of growth and death kinetics at single-cell resolution is a key step in understanding the complexity of the nonreplicating growth phenotype of the bacterial pathogen Mycobacterium tuberculosis. Here, we developed a single-cell-resolution microfluidic mycobacterial culture device that allows time-lapse microscopy-based long-term phenotypic visualization of the live replication dynamics of mycobacteria. This technology was successfully applied to monitor the real-time growth dynamics of the fast-growing model strain Mycobacterium smegmatis (M. smegmatis) while subjected to drug treatment regimens during continuous culture for 48 h inside the microfluidic device. A clear morphological change leading to significant swelling at the poles of the bacterial membrane was observed during drug treatment. In addition, a small subpopulation of cells surviving treatment by frontline antibiotics was observed to recover and achieve robust replicative growth once regular culture media was provided, suggesting the possibility of identifying and isolating nonreplicative mycobacteria. This device is a simple, easy-to-use, and low-cost solution for studying the single-cell phenotype and growth dynamics of mycobacteria, especially during drug treatment.

scholarly journals “CLUPS”: A New Culture Medium for the Axenic Growth ofEntamoeba histolytica

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
F. Gonzalez-Salazar ◽  
I. Meester ◽  
F. J. Guzmán De La Garza ◽  
L. H. De La Garza-Salinas ◽  
A. Sampayo-Reyes ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Low Cost ◽  
The Novel ◽  
Phospholipase Activity ◽  
Major Health Problem ◽  
Beef Liver ◽  
Major Health ◽  
Liver Abscesses ◽  
Therapeutic Tools

Amebiasis remains a major health problem in Mexico. Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental. We present a new culture medium forEntamoeba histolyticawhich allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models. The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory. The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium. To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS),E. histolyticatrophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters.E. histolyticatrophozoites grew significantly better in CLUPS medium than in TYI-S-33. Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites. Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.

scholarly journals Exploiting the Sequence of Naturally Occurring Elastin: Construction, Production and Characterization of a Recombinant Thermoplastic Protein-Based Polymer

2009 ◽  
Vol 6 ◽  
pp. 133-145 ◽  
Author(s):  
Raul Machado ◽  
A.J. Ribeiro ◽  
J. Padrão ◽  
D. Silva ◽  
A. Nobre ◽  
...  
Keyword(s):  
Culture Media ◽  
Low Cost ◽  
Carbon Sources ◽  
Molecular Weight Polymer ◽  
Precise Control ◽  
Self Assembling ◽  
Thermoplastic Protein ◽  
Optimization Of Fermentation ◽  
Absolute Control

Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologically produced in Escherichia coli and purified by hot and cold centrifugation cycles, based on the reversible inverse temperature transition property of ELPs. The use of low cost carbon sources like lactose and glycerol for bacteria cells culture media was explored using Central Composite Design approach allowing optimization of fermentation conditions. Due to its self-assembling behaviour near 33 °C stable spherical microparticles with a size ~ 1µm were obtained, redissolving when a strong undercooling is achieved. The polymer produced showed hysteresis behaviour with thermal absorbing/releasing components depending on the salt concentration of the polymer solution.

PAH removal by immobilized bacterial cells-support systems using low-cost culture media for biomass production

2017 ◽  
Vol 120 ◽  
pp. 6-14 ◽  
Author(s):  
Mauricio J. Alessandrello ◽  
María S. Juárez Tomás ◽  
Paula Isaac ◽  
Diana L. Vullo ◽  
Marcela A. Ferrero
Keyword(s):  
Biomass Production ◽  
Support Systems ◽  
Culture Media ◽  
Low Cost ◽  
Bacterial Cells

Aspects of Carbon Dioxide Mitigation by Nannochloropsis oculata Cultured in a Photobioreactor

2014 ◽  
Vol 625 ◽  
pp. 775-779
Author(s):  
Vijendren Krishnan ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Norridah Osman
Keyword(s):  
Carbon Dioxide ◽  
Carbon Dioxide Emission ◽  
Lipid Synthesis ◽  
Carbon Dioxide Concentration ◽  
Nannochloropsis Oculata ◽  
Marine Microalgae ◽  
Biodiesel Production ◽  
Photobioreactor Design ◽  
Reduce Carbon Dioxide ◽  
Carbon Dioxide Mitigation

This paper primarily presents on carbon dioxide mitigation by marine microalgae. Among the potential marine microalgae,Nannochloropsis oculatahas been identified as a promising species which can be utilized to reduce carbon dioxide concentration via photosynthesis process. The growth ofN. oculataand lipid synthesis for biodiesel production is influenced by various aspects. The aspects that are focused in this paper include light source and intensity, temperature, carbon dioxide concentration, and photobioreactor design. Besides, emerging technologies that are able to increase the efficiency of carbon dioxide reduction were also highlighted. As a whole,N. oculatamight play a key role in reducing the global carbon dioxide emission as well as enhancing the biodiesel production.

A low-cost antifoam additive for agar-based culture media

1987 ◽  
Vol 63 (5) ◽  
pp. 465-468
Author(s):  
J. I. Blenkharn ◽  
Sharon Wood
Keyword(s):  
Culture Media ◽  
Low Cost
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