scholarly journals Bacterial contamination of surgical scrub suits worn outside the operating theatre: a randomised crossover study

Anaesthesia ◽  
2014 ◽  
Vol 69 (8) ◽  
pp. 816-825 ◽  
Author(s):  
H. I. Hee ◽  
S. Lee ◽  
S. N. Chia ◽  
Q. S. Lu ◽  
A. P. Q. Liew ◽  
...  
2019 ◽  
Vol 41 (1) ◽  
pp. 120-123
Author(s):  
Patricia Sebastian Marcos ◽  
Darren Hermes ◽  
Mellora Sharman

AbstractIn a crossover study, 30 stethoscopes were assessed and disinfected using 3 protocols: isopropyl alcohol, a quaternary ammonia or biguanide disinfectant, and ultraviolet germicidal irradiation (UVGI). All protocols effectively reduced bacterial loads, but UVGI was less effective at higher contamination levels (P = .0004). The effectiveness of each intervention was short in duration.


2018 ◽  
Vol 4 (1) ◽  
pp. 243-245 ◽  
Author(s):  
Sabine Gruber ◽  
Sebastian Buhl ◽  
Clemens Bulitta

AbstractThe purpose of this work was to evaluate the decontamination potential of the Potok system both in an experimental setting in a research Operating Room (OR) with standalone Air Decontamination Units (Potok 150-M-01) and in a clinical setting in a real operating theatre in Moscow. Our experiments showed an impact of the Potok units on the bacterial contamination of the room air according to the Swedish SIS-TS 39:2015 standard. For the initial measurements in our research OR in Weiden this could be shown by a decrease of the bacterial burden at all three different measurement points (OR table, instrumentation tray, periphery). Also the subsequently done measurements in the Moscow hospital verified this decontaminating effectivity of the Potok system. In this case the initial background contamination of the operating theatre was higher than in the research OR in Germany. This bacterial burden could be effectively decreased by the use of the installed Potok based ventilation system.


1969 ◽  
Vol 67 (3) ◽  
pp. 417-425 ◽  
Author(s):  
G. A. J. Ayliffe ◽  
J. R. Babb ◽  
B. J. Collins ◽  
E. J. L. Lowbury

SUMMARYThe value of clean zones and of transfer areas in operating suites was assessed by comparisons of the amounts of contamination on floors, trolleys and footwear in suites with and without a clean zone and a transfer area; counts of Clostridium welchii were used as an index of bacterial contamination introduced into the aseptic zone from outside.The mean counts of Cl. welchii on contact plates from the wheels of trolleys used to convey patients from wards to the operating suite (67·9 ± 7·68 per plate) were significantly higher than those from theatre trolleys (i.e. those used only inside a theatre suite provided with a transfer area) (3·13 ± 0·47 per plate); mean counts of total bacteria were only slightly lower on the wheels of theatre trolleys than on those of hospital trolleys. Other surfaces of hospital trolleys showed counts similar to those found on theatre trolleys.Contact plates from floors showed significantly lower counts of Cl. welchii in the aseptic zone and the clean zone than in the hospital corridor, the protective zone and (when present) the transfer area.The mean counts per 100 cm2 of Cl. welchii were approximately the same on the floor of a theatre with a clean zone and a transfer area (0·83) as in one with a clean zone but no transfer area (0·5). Counts of total bacteria were higher in the latter. A suite with no clean zone or transfer area showed a higher mean count of Cl. welchii on contact plates from the aseptic zone (operating theatre) (20·5 ± 12·33 per 100 cm2). These higher levels of contamination were due to sporadic high counts of Cl. welchii found near the door of the theatre with no clean zone; in another theatre with no clean zone the level of Cl. welchii on the floor was not higher than that in the theatres with clean zones.Theatres with plenum ventilation had lower mean counts of airborne Cl. welchii than those ventilated by windows: there was no significant difference in the levels of Cl. welchii on the floors of theatres with the two forms of ventilation.On sampling with contact plates, theatre footwear yielded fewer total organisms, Staphylococcus aureus and Cl. welchii than outdoor shoes removed before entering the clean zone.The hygienic value of transfer areas and clean zones is discussed. Bacteriological support could not be obtained for the former, but the latter appeared to contribute something to the cleanliness of the theatre by preventing heavy sporadic contamination.We wish to thank Mr M. Wilkins for valuable assistance, the staff of the operating theatres for their co-operation and Alne Engineering Limited, 57 High Street, Henley-in-Arden, Solihull, for supplying disposable contact plates.


2014 ◽  
Vol 35 (11) ◽  
pp. 1411-1413 ◽  
Author(s):  
Mallory A. Boutin ◽  
Kerri A. Thom ◽  
Min Zhan ◽  
J. Kristie Johnson

Healthcare worker attire may become contaminated with pathogenic organisms during a normal shift. We performed a randomized crossover study to assess whether treatment with an antimicrobial coating would decrease bacterial contamination on scrubs. Thirty percent of all scrubs were contaminated; there was no difference in the rate of contamination between the intervention and control groupsInfect Control Hosp Epidemiol 2014;35(11):1411–1413


1984 ◽  
Vol 5 (7) ◽  
pp. 339-341 ◽  
Author(s):  
Britt Marie Andersson ◽  
Lars Lidgren ◽  
Claës Schalén ◽  
Anita Steen

AbstractSterile irrigation solutions, used in open systems in a conventional orthopedic operating theatre, were examined for bacterial contamination. At the end of 13 of 21 operations (duration ≥ one hour) the solution tested displayed the growth of Staphylococcus epidermidis and/or diphtheroid rods and/or other species. It is concluded that irrigation by solutions, kept in open bowls during an operation, is a potential source of contamination.


2018 ◽  
Vol 4 (1) ◽  
pp. 177-179
Author(s):  
Sabine Gruber ◽  
Sebastian Buhl ◽  
Clemens Bulitta

AbstractThe purpose of this work was to evaluate the decontamination potential of the Potok system both in an experimental setting in a research Operating Room (OR) with standalone Air Decontamination Units (Potok 150-M-01) and in a clinical setting in a real operating theatre in Moscow. Our experiments showed an impact of the Potok units on the bacterial contamination of the room air according to the Swedish SIS-TS 39:2015 standard. For the initial measurements in our research OR in Weiden this could be shown by a decrease of the bacterial burden at all three different measurement points (OR table, instrumentation tray, periphery). Also the subsequently done measurements in the Moscow hospital verified this decontaminating effectivity of the Potok system. In this case the initial background contamination of the operating theatre was higher than in the research OR in Germany. This bacterial burden could be effectively decreased by the use of the installed Potok based ventilation system.


2019 ◽  
Vol 3 (4) ◽  
pp. 75-76
Author(s):  
Dito Anggoro ◽  
Setyo Budhi ◽  
Agus Purnomo ◽  
Dorothea Vera Megarani

Ultraviolet (UV) lamp is the simplest method for sterilizing operating theatre. This method is effective, easily operated, and does not require high cost. Furthermore, there were several studies of microorganism contamination in the air and surface at human operating theatre. However, studies in veterinary operating theatre related to the effectiveness of UV light on sterilization process is still limited, especially in Indonesia. Bacterial contamination samples were collected three times each in three different conditions: A) before surgery and without UV, B) before surgery but UV was already used, and C) after surgery and UV was already used. Samples were taken with settle plate and swab method for collecting the air and operating table contamination, respectively. One-way repeated measures ANOVA determined that there was statistically significant difference in the number of bacterial contaminations between three conditions (A, B, and C) in settle plate method (p=0.009), as well as in swab method (p=0.010). The result revealed that the UV light was effective to sterilize operating theatre, which can be seen from the significant decreases on the number of bacterial contaminations before and after the UV was used, both in settle plate and swab method. The result of this study supported the theory that the UV light can reduce the air bacterial and surface contamination at operating theatre. However, the result of microorganism contaminations in this study was still not appropriate based on the standard minimum of total bacterial in the operating theatre from The Ministry of Health, Republic of Indonesia. Consequently, the use of another method of sterilization at the operating theatre is still required for a better sterilization result.


1978 ◽  
Vol 80 (1) ◽  
pp. 57-67 ◽  
Author(s):  
A. Hambraeus ◽  
S. Bengtsson ◽  
G. Laurell

SUMMARYIn this investigation the bacterial contamination of surfaces such as walls and floors in a modern operating suite, together with surfaces of lamps in the operating theatres, and the clogs worn by staff, was studied. Counts of colony-forming units were made on impression plates containing blood-agar with Tween 80 for total bacterial counts, Baird Parker medium with egg yolk and tellurite for Staphylococcus aureus and trypticase peptone agar with neomycin and polymyxin for Clostridium spp.The areas examined were divided into the patients' route to the operating theatre, the staff's route, and the central area containing the operating rooms, anaesthetic rooms, and exit and scrub-up areas. In the patients' route counts of total organisms ranged from about 10 000 to 30 000/m2 for Staph. aureus the range was from 70 to 540/m2. In the staff's route the highest count was about 70000/m2 in the dressing area, and the numbers of Staph. aureus were about the same as along the patients' route. In the inner zone the counts were somewhat lower for both total organisms and Staph. aureus. Total counts on the floor from all areas of the inner zone were significantly higher just before the second operation than before the first operation on the same day. The total and Staph. aureus counts on walls, floors and lamps were the same after clean operations as after operations classified as ‘contaminated’ or ‘dirty’.


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