The impact of platelet‐rich fibrin (PRF) on delayed tooth replantation: A preliminary animal study

2021 ◽  
Author(s):  
Mohammad Behnaz ◽  
Seyed Sadra Izadi ◽  
Fatemeh Mashhadi Abbas ◽  
Omid Dianat ◽  
Sina Sadeghabadi ◽  
...  
Keyword(s):  
Animal Study ◽  
Platelet Rich Fibrin ◽  
Tooth Replantation ◽  
The Impact

scholarly journals Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3466
Author(s):  
Zahra Kargarpour ◽  
Jila Nasirzade ◽  
Layla Panahipour ◽  
Richard J. Miron ◽  
Reinhard Gruber
Keyword(s):  
Target Genes ◽  
Nuclear Translocation ◽  
Kinase Inhibitor ◽  
Plasma Layer ◽  
Buffy Coat ◽  
Type I ◽  
Platelet Rich Fibrin ◽  
Nadph Oxidase 4 ◽  
Coat Layer ◽  
The Impact

Liquid platelet-rich fibrin (PRF) can be prepared by high centrifugation forces separating the blood into a platelet-poor plasma (PPP) layer and a cell-rich buffy coat layer, termed concentrated PRF (C-PRF). Heating the liquid PPP was recently introduced to prepare an albumin gel (Alb-gel) that is later mixed back with the concentrated liquid C-PRF to generate Alb-PRF. PRF is a rich source of TGF-β activity; however, the overall TGF-β activity in the PPP and the impact of heating the upper plasma layer remains unknown. Here, we investigated for the first time the in vitro TGF-β activity of all fractions of Alb-PRF. We report that exposure of oral fibroblasts with lysates of PPP and the buffy coat layer, but not with heated PPP, provoked a robust increase in the TGF-β target genes interleukin 11 and NADPH oxidase 4 by RT-PCR, and for IL11 by immunoassay. Consistent with the activation of TGF-β signaling, expression changes were blocked in the presence of the TGF-β receptor type I kinase inhibitor SB431542. Immunofluorescence and Western blot further confirmed that lysates of PPP and the buffy coat layer, but not heated PPP, induced the nuclear translocation of Smad2/3 and increased phosphorylation of Smad3. The immunoassay further revealed that PPP and particularly BC are rich in active TGF-β compared to heated PPP. These results strengthen the evidence that not only the cell-rich C-PRF but also PPP comprise a TGF-β activity that is, however, heat sensitive. It thus seems relevant to mix the heated PPP with the buffy coat C-PRF layer to regain TGF-β activity, as proposed during the preparation of Alb-PRF.

Histological evaluation of effectiveness of platelet-rich fibrin on healing of sinus membrane perforations: A preclinical animal study

2017 ◽  
Vol 45 (8) ◽  
pp. 1150-1157 ◽  
Author(s):  
Ceyhun Aricioglu ◽  
Dogan Dolanmaz ◽  
Alparslan Esen ◽  
Kubilay Isik ◽  
Mustafa Cihat Avunduk
Keyword(s):  
Animal Study ◽  
Histological Evaluation ◽  
Platelet Rich Fibrin ◽  
Preclinical Animal Study ◽  
Sinus Membrane

The impact of platelet-rich fibrin (PRF) on olfactory function and pain after septoplasty operations

2020 ◽  
Vol 277 (4) ◽  
pp. 1115-1120 ◽  
Author(s):  
Belgin Tutar ◽  
Enis Ekincioglu ◽  
Semih Karaketir ◽  
Güler Berkiten ◽  
Ziya Saltürk ◽  
...  
Keyword(s):  
Olfactory Function ◽  
Platelet Rich Fibrin ◽  
The Impact

scholarly journals Synergistic effects of the combined use of human-cultured periosteal sheets and platelet-rich fibrin on bone regeneration: An animal study

2017 ◽  
Vol 3 (4) ◽  
pp. 134-141 ◽  
Author(s):  
Makoto Horimizu ◽  
Takehiko Kubota ◽  
Tomoyuki Kawase ◽  
Masaki Nagata ◽  
Mito Kobayashi ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Animal Study ◽  
Synergistic Effects ◽  
Platelet Rich Fibrin ◽  
Combined Use

scholarly journals Impact of g force and timing on the characteristics of platelet-rich fibrin matrices

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana B. Castro ◽  
C. Andrade ◽  
X. Li ◽  
N. Pinto ◽  
W. Teughels ◽  
...  
Keyword(s):  
Growth Factors ◽  
In Vitro Study ◽  
Blood Draw ◽  
Minimal Impact ◽  
Platelet Rich Fibrin ◽  
Cellular Content ◽  
Beneficial Impact ◽  
The Difference ◽  
The Impact

AbstractRecently, new centrifugation protocols for the preparation of platelet-rich fibrin (PRF) have been introduced in an attempt to further improve the beneficial impact of these 2nd generation platelet concentrate membranes. This in-vitro study aimed to compare the biological and physical characteristics of three types of PRF membranes using two different centrifuges with adapted relative centrifugal forces (RCF): leucocyte- and platelet-rich fibrin, advanced platelet-rich fibrin, and advanced platelet-rich fibrin+. Release of growth factors, macroscopic dimensions, cellular content and mechanical properties of the respective membranes, prepared from blood of the same individual were explored. Furthermore, the impact of timing (blood draw-centrifugation and centrifugation-membrane preparation) was assessed morphologically as well as by electron microscopy scanning. No statistically significant differences amongst the three PRF modifications could be observed, neither in their release of growth factors or the cellular content, nor in clot/membrane dimensions. The difference between both centrifuges were negligible when the same g-force was used. A lower g-force, however, reduced membrane tensile strength. Timing in the preparation process had a significant impact. Adaptation of RCF only had a minimal impact on the final characteristics of PRF membranes.

The impact of immobilization and strength exercise strategy to bone change: An animal study

Bone ◽  
2008 ◽  
Vol 43 ◽  
pp. S116
Author(s):  
Tachung Lee ◽  
Chingyen Lin ◽  
Teenmeei Wang
Keyword(s):  
Animal Study ◽  
Strength Exercise ◽  
Bone Change ◽  
The Impact

scholarly journals Does Platelet-Rich Fibrin Decrease Dimensional Changes and Improve Postoperative Comfort in Post-Extraction Sockets? An Overview of Systematic Reviews

2020 ◽  
Vol 10 (17) ◽  
pp. 5750 ◽  
Author(s):  
Vittorio Moraschini ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
Rafael Coutinho de Mello Machado ◽  
Jhonathan Raphaell Barros Nascimento ◽  
Kayvon Javid ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Systematic Reviews ◽  
Selection Process ◽  
Soft Tissue Healing ◽  
Socket Preservation ◽  
Platelet Rich Fibrin ◽  
Dimensional Changes ◽  
Tissue Healing ◽  
The Impact

This overview aimed to evaluate the methods, quality, and outcomes of systematic reviews (SRs) conducted to investigate the effects of platelet-rich fibrin (PRF) in dental sockets on promoting bone regeneration and soft tissue healing and diminishing the incidence of pain, swelling, trismus, and alveolar osteitis after tooth extraction. An electronic search without date or language restriction was done in PubMed/MEDLINE, Cochrane, and Web of Science until March 2020. Eligibility criteria included SRs that assessed the effect of PRF for human alveolar socket preservation. The quality assessment of the included studies was performed using AMSTAR 2 guidelines. The protocol of this overview was recorded in PROSPERO under the number CRD42018089617. The search and selection process yielded 13 studies published between 2011 and 2018. The analysis of the studies showed inconclusive data for the effect of the PRF and the dimensional changes. There is no definitive evidence for the impact of using PRF alone on bone regeneration in post-extraction sockets. The use of PRF improves soft tissue healing and reduces pain, bleeding, and osteitis in post-extraction sockets.

Evaluation of Advanced Platelet Rich Fibrin (A-PRF) on Bone Healing. Is It Better than Old Version? A Histological Animal Study

2017 ◽  
Vol 7 (6) ◽  
pp. 478-483 ◽  
Author(s):  
Kübra Titirinli ◽  
Umut Tekin ◽  
Fethi Atıl ◽  
M. Ercüment Önder ◽  
Burcu Şenguven ◽  
...  
Keyword(s):  
Animal Study ◽  
Bone Healing ◽  
Platelet Rich Fibrin ◽  
Better Than
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