scholarly journals Inhibitory transmission in the bed nucleus of the stria terminalis in male and female mice following morphine withdrawal

2019 ◽  
Vol 25 (3) ◽  
Author(s):  
Brennon R. Luster ◽  
Elizabeth S. Cogan ◽  
Karl T. Schmidt ◽  
Dipanwita Pati ◽  
Melanie M. Pina ◽  
...  
Keyword(s):  
Morphine Withdrawal ◽  
Stria Terminalis ◽  
Male And Female ◽  
Bed Nucleus ◽  
Inhibitory Transmission ◽  
Female Mice

scholarly journals Male and female mice demonstrate divergent cellular responses in the bed nucleus of stria terminalis (BNST) following morphine withdrawal

2018 ◽  
Author(s):  
Brennon R. Luster ◽  
Elizabeth S. Cogan ◽  
Karl T. Schmidt ◽  
Dipanwita Pati ◽  
Melanie M. Pina ◽  
...  
Keyword(s):  
Opioid Analgesics ◽  
Cellular Responses ◽  
The United States ◽  
Morphine Withdrawal ◽  
Stria Terminalis ◽  
Male Mice ◽  
Male And Female ◽  
Bed Nucleus ◽  
Precipitated Withdrawal ◽  
Female Mice

AbstractThe United States is experiencing an opioid epidemic of significant proportions, imposing enormous fiscal and societal costs. While prescription opioid analgesics are essential for treating pain, the cessation of these drugs can induce a withdrawal syndrome, and thus opioid use often persists to alleviate or avoid these symptoms. Therefore, it is essential to understand the neurobiology underlying this critical window of withdrawal from opioid analgesics to prevent continued usage. To model this, we administered a low dose of morphine, and precipitated withdrawal with naloxone to investigate the behavioral and cellular responses in C57BL/6J male and female mice. Following 3 days of administration, both male and female mice sensitized to the repeated bouts of withdrawal, as evidenced by their composite global withdrawal score. Female mice exhibited increased withdrawal symptoms on some individual measures, but did not show characteristic weight loss observed in male mice. Because of its role in mediating withdrawal-associated behaviors, we examined neuronal excitability and inhibitory synaptic transmission in the bed nucleus of the stria terminalis (BNST) 24 hours following the final precipitated withdrawal. In male mice, morphine withdrawal increased spontaneous GABAergic signaling compared to controls. In contrast, morphine withdrawal decreased spontaneous GABAergic signaling, and increased BNST projection neuron excitability in female mice. Intriguingly, these opposing GABAergic effects were dependent on within slice excitability. Our findings suggest that male and female mice manifest divergent cellular responses in the BNST following morphine withdrawal, and alterations in BNST inhibitory signaling may be a significant factor contributing to the expression of behaviors following opioid withdrawal.

scholarly journals Corticotropin-Releasing Factor Neurons in the Bed Nucleus of the Stria Terminalis Differentially Influence Pain Processing and Modulation in Male and Female Mice

2020 ◽  
Author(s):  
Waylin Yu ◽  
Christina M. Stanhope ◽  
Natalia del R. Rivera Sanchez ◽  
Garrett A. Moseley ◽  
Thomas L. Kash
Keyword(s):  
Pain Modulation ◽  
Corticotropin Releasing Factor ◽  
Stria Terminalis ◽  
Noxious Heat ◽  
Specific Expression ◽  
Male And Female ◽  
Bed Nucleus ◽  
Female Mice ◽  
Pain Knowledge

AbstractThe bed nucleus of the stria terminalis (BNST) plays an emerging yet understudied role in pain. Corticotropin-releasing factor (CRF) is an important source of pain modulation in the BNST, with local pharmacological inhibition of CRF receptors conditionally impacting the sensory and affective-motivational components of pain. Knowledge on how pain dynamically engages CRF neurons in the BNST and is influenced by intra-BNST production of CRF, however, remains unknown. In the present study, we utilized in vivo calcium imaging to show robust and synchronized recruitment of BNSTCRF+ neurons during acute exposure to noxious heat. Distinct patterns of recruitment were observed by sex, as the magnitude and timing of heat responsive activity in BNSTCRF+ neurons differed for male and female mice. We then established the necessity of CRF for intact pain behaviors by genetically deleting Crf in the BNST, which reduced thermal and mechanical nociceptive sensitivity for both sexes, and increased paw attending in female mice, suggesting a divergent role for CRF with respect to active coping responses to pain. Together, these findings demonstrate that CRF in the BNST contributes to multiple facets of the pain experience and may play a key role in the sex-specific expression of pain-related behaviors.

Morphine withdrawal syndrome and its prevention with baclofen: Autoradiographic study of μ-opioid receptors in prepubertal male and female mice

Synapse ◽  
2006 ◽  
Vol 60 (2) ◽  
pp. 132-140 ◽  
Author(s):  
Silvina L. Diaz ◽  
Virginia G. Barros ◽  
Marta C. Antonelli ◽  
Modesto C. Rubio ◽  
Graciela N. Balerio
Keyword(s):  
Opioid Receptors ◽  
Withdrawal Syndrome ◽  
Autoradiographic Study ◽  
Morphine Withdrawal ◽  
Male And Female ◽  
Female Mice ◽  
Μ Opioid Receptors

Morphine Withdrawal Reactions in Male and Female Mice

1976 ◽  
Vol 3 (2) ◽  
pp. 363-368 ◽  
Author(s):  
Kenneth Blum ◽  
Joseph D. Eubanks ◽  
Ben Wiggins ◽  
Jack E. Wallace
Keyword(s):  
Morphine Withdrawal ◽  
Male And Female ◽  
Female Mice
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