scholarly journals Knockdown of Sexually Differentiated Vasopressin Expression in the Bed Nucleus of the Stria Terminalis Reduces Social and Sexual Behavior in Male, but Not Female, Mice

2021 ◽  
Author(s):  
Nicole Rigney ◽  
Adam Zbib ◽  
Geert J. Vries ◽  
Aras Petrulis
Keyword(s):  
Sexual Behavior ◽  
Stria Terminalis ◽  
Bed Nucleus ◽  
Female Mice

scholarly journals Male and female mice demonstrate divergent cellular responses in the bed nucleus of stria terminalis (BNST) following morphine withdrawal

2018 ◽  
Author(s):  
Brennon R. Luster ◽  
Elizabeth S. Cogan ◽  
Karl T. Schmidt ◽  
Dipanwita Pati ◽  
Melanie M. Pina ◽  
...  
Keyword(s):  
Opioid Analgesics ◽  
Cellular Responses ◽  
The United States ◽  
Morphine Withdrawal ◽  
Stria Terminalis ◽  
Male Mice ◽  
Male And Female ◽  
Bed Nucleus ◽  
Precipitated Withdrawal ◽  
Female Mice

AbstractThe United States is experiencing an opioid epidemic of significant proportions, imposing enormous fiscal and societal costs. While prescription opioid analgesics are essential for treating pain, the cessation of these drugs can induce a withdrawal syndrome, and thus opioid use often persists to alleviate or avoid these symptoms. Therefore, it is essential to understand the neurobiology underlying this critical window of withdrawal from opioid analgesics to prevent continued usage. To model this, we administered a low dose of morphine, and precipitated withdrawal with naloxone to investigate the behavioral and cellular responses in C57BL/6J male and female mice. Following 3 days of administration, both male and female mice sensitized to the repeated bouts of withdrawal, as evidenced by their composite global withdrawal score. Female mice exhibited increased withdrawal symptoms on some individual measures, but did not show characteristic weight loss observed in male mice. Because of its role in mediating withdrawal-associated behaviors, we examined neuronal excitability and inhibitory synaptic transmission in the bed nucleus of the stria terminalis (BNST) 24 hours following the final precipitated withdrawal. In male mice, morphine withdrawal increased spontaneous GABAergic signaling compared to controls. In contrast, morphine withdrawal decreased spontaneous GABAergic signaling, and increased BNST projection neuron excitability in female mice. Intriguingly, these opposing GABAergic effects were dependent on within slice excitability. Our findings suggest that male and female mice manifest divergent cellular responses in the BNST following morphine withdrawal, and alterations in BNST inhibitory signaling may be a significant factor contributing to the expression of behaviors following opioid withdrawal.

scholarly journals A Sexually Dimorphic Area of the Dorsal Hypothalamus in Mice and Common Marmosets

Endocrinology ◽  
2016 ◽  
Vol 157 (12) ◽  
pp. 4817-4828 ◽  
Author(s):  
Yadanar Moe ◽  
Chaw Kyi-Tha-Thu ◽  
Tomoko Tanaka ◽  
Hiroto Ito ◽  
Satowa Yahashi ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Sexual Behaviors ◽  
Maternal Behavior ◽  
Neuronal Cell ◽  
Sexually Dimorphic ◽  
Common Marmosets ◽  
Female Sexual Behavior ◽  
Bed Nucleus ◽  
Female Mice ◽  
Activity Marker

We found a novel sexually dimorphic area (SDA) in the dorsal hypothalamus (DH) of mice. The SDA-DH was sandwiched between 2 known male-biased sexually dimorphic nuclei, the principal nucleus of the bed nucleus of the stria terminalis and the calbindin-sexually dimorphic nucleus, and exhibited a female-biased sex difference in neuronal cell density. The density of neurons in the SDA-DH was increased in male mice by orchidectomy on the day of birth and decreased in female mice by treatment with testosterone, dihydrotestosterone, or estradiol within 5 days after birth. These findings indicate that the SDA-DH is defeminized under the influence of testicular testosterone, which acts via both directly by binding to the androgen receptor, and indirectly by binding to the estrogen receptor after aromatization. We measured the activity of SDA-DH neurons with c-Fos, a neuronal activity marker, in female mice during maternal and sexual behaviors. The number of c-Fos-expressing neurons in the SDA-DH of female mice was negatively correlated with maternal behavior performance. However, the number of c-Fos-expressing neurons did not change during female sexual behavior. These findings suggest that the SDA-DH contains a neuronal cell population, the activity of which decreases in females exhibiting higher performance of maternal behavior, but it may contribute less to female sexual behavior. Additionally, we examined the brain of common marmosets and found an area that appears to be homologous with the mouse SDA-DH. The sexually dimorphic structure identified in this study is not specific to mice and may be found in other species.

scholarly journals Inhibitory transmission in the bed nucleus of the stria terminalis in male and female mice following morphine withdrawal

2019 ◽  
Vol 25 (3) ◽  
Author(s):  
Brennon R. Luster ◽  
Elizabeth S. Cogan ◽  
Karl T. Schmidt ◽  
Dipanwita Pati ◽  
Melanie M. Pina ◽  
...  
Keyword(s):  
Morphine Withdrawal ◽  
Stria Terminalis ◽  
Male And Female ◽  
Bed Nucleus ◽  
Inhibitory Transmission ◽  
Female Mice

scholarly journals Corticotropin-Releasing Factor Neurons in the Bed Nucleus of the Stria Terminalis Differentially Influence Pain Processing and Modulation in Male and Female Mice

2020 ◽  
Author(s):  
Waylin Yu ◽  
Christina M. Stanhope ◽  
Natalia del R. Rivera Sanchez ◽  
Garrett A. Moseley ◽  
Thomas L. Kash
Keyword(s):  
Pain Modulation ◽  
Corticotropin Releasing Factor ◽  
Stria Terminalis ◽  
Noxious Heat ◽  
Specific Expression ◽  
Male And Female ◽  
Bed Nucleus ◽  
Female Mice ◽  
Pain Knowledge

AbstractThe bed nucleus of the stria terminalis (BNST) plays an emerging yet understudied role in pain. Corticotropin-releasing factor (CRF) is an important source of pain modulation in the BNST, with local pharmacological inhibition of CRF receptors conditionally impacting the sensory and affective-motivational components of pain. Knowledge on how pain dynamically engages CRF neurons in the BNST and is influenced by intra-BNST production of CRF, however, remains unknown. In the present study, we utilized in vivo calcium imaging to show robust and synchronized recruitment of BNSTCRF+ neurons during acute exposure to noxious heat. Distinct patterns of recruitment were observed by sex, as the magnitude and timing of heat responsive activity in BNSTCRF+ neurons differed for male and female mice. We then established the necessity of CRF for intact pain behaviors by genetically deleting Crf in the BNST, which reduced thermal and mechanical nociceptive sensitivity for both sexes, and increased paw attending in female mice, suggesting a divergent role for CRF with respect to active coping responses to pain. Together, these findings demonstrate that CRF in the BNST contributes to multiple facets of the pain experience and may play a key role in the sex-specific expression of pain-related behaviors.

Amygdaloid lesion-induced obesity: relation to sexual behavior, olfaction, and the ventromedial hypothalamus

2006 ◽  
Vol 291 (5) ◽  
pp. R1201-R1214 ◽  
Author(s):  
Bruce M. King
Keyword(s):  
Sexual Behavior ◽  
Nodal Point ◽  
Ventromedial Hypothalamus ◽  
Female Rats ◽  
Male Rats ◽  
Amygdaloid Nucleus ◽  
Stria Terminalis ◽  
Bed Nucleus ◽  
Normal Feeding ◽  
Medial Amygdaloid Nucleus

Lesions of the amygdala have long been known to produce hyperphagia and obesity in cats, dogs, and monkeys, but only recently have studies with rats determined that the effective site is the posterodorsal amygdala (PDA)—the posterodorsal medial amygdaloid nucleus and the intra-amygdaloid bed nucleus of the stria terminalis. There is a sex difference; female rats with PDA lesions display greater weight gain than male rats. In the brains of female rats with obesity-inducing PDA lesions, there is a dense pattern of axonal degeneration in the capsule of the ventromedial hypothalamus (VMH) and other targets of the stria terminalis. Transections of the dorsal component of the stria terminalis also result in hyperphagia and obesity in female rats. Similar to rats with VMH lesions, rats with PDA lesions are hyperinsulinemic during food restriction and greatly prefer high-carbohydrate diets. The PDA is also a critical site for some aspects of rodent sexual behavior, particularly those that depend on olfaction, and the pattern of degeneration observed after obesity-inducing PDA lesions is remarkably parallel to the circuit that has been proposed to mediate sexual behavior. Medial amygdaloid lesions disrupt the normal feeding pattern and result in impaired responses to caloric challenges, and there is evidence that these behavioral changes are also due to a disruption of olfactory input. With its input from the olfactory bulbs and connections to the VMH, the PDA may be a nodal point at which olfactory and neuroendocrine stimuli are integrated to affect feeding behavior.

Author response for "Projections from the Dorsomedial Division of the Bed Nucleus of the Stria Terminalis to Hypothalamic Nuclei in the Mouse"

2020 ◽  
Author(s):  
Marie Barbier ◽  
J. Antonio González ◽  
Christophe Houdayer ◽  
Denis Burdakov ◽  
Pierre‐Yves Risold ◽  
...  
Keyword(s):  
Author Response ◽  
Stria Terminalis ◽  
Bed Nucleus ◽  
Hypothalamic Nuclei
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