scholarly journals Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: Function of the Asn-His interaction in the catalytic triad

2001 ◽  
Vol 10 (10) ◽  
pp. 1962-1969 ◽  
Author(s):  
H.J. Snijder ◽  
J.H. Van Eerde ◽  
R.L. Kingma ◽  
K.H. Kalk ◽  
N. Dekker ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Active Site ◽  
Catalytic Triad ◽  
Phospholipase A ◽  
Structural Investigations ◽  
Outer Membrane Phospholipase A

scholarly journals Half-of-the-sites reactivity of outer-membrane phospholipase A against an active-site-directed inhibitor

1999 ◽  
Vol 260 (3) ◽  
pp. 794-800 ◽  
Author(s):  
Iban Ubarretxena-Belandia ◽  
Ruud C. Cox ◽  
Ruud Dijkman, ◽  
Maarten R. Egmond, ◽  
Hubertus M. Verheij ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Active Site ◽  
Phospholipase A ◽  
Outer Membrane Phospholipase A

scholarly journals Absence of the Outer Membrane Phospholipase A Suppresses the Temperature-Sensitive Phenotype of Escherichia coli degPMutants and Induces the Cpx and ςE Extracytoplasmic Stress Responses

2001 ◽  
Vol 183 (18) ◽  
pp. 5230-5238 ◽  
Author(s):  
Geoffrey R. Langen ◽  
Jill R. Harper ◽  
Thomas J. Silhavy ◽  
S. Peter Howard
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Stress Responses ◽  
Elevated Temperatures ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Phospholipase A ◽  
Temperature Sensitive ◽  
Outer Membrane Phospholipase A ◽  
Temperature Sensitive Phenotype

ABSTRACT DegP is a periplasmic protease that is a member of both the ςE and Cpx extracytoplasmic stress regulons ofEscherichia coli and is essential for viability at temperatures above 42°C. [U-14C]acetate labeling experiments demonstrated that phospholipids were degraded indegP mutants at elevated temperatures. In addition, chloramphenicol acetyltransferase, β-lactamase, and β-galactosidase assays as well as sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that large amounts of cellular proteins are released from degP cells at the nonpermissive temperature. A mutation in pldA, which encodes outer membrane phospholipase A (OMPLA), was found to rescue degPcells from the temperature-sensitive phenotype. pldA degP mutants had a normal plating efficiency at 42°C, displayed increased viability at 44°C, showed no degradation of phospholipids, and released far lower amounts of cellular protein to culture supernatants. degP and pldA degP mutants containing chromosomal lacZ fusions to Cpx and ςE regulon promoters indicated that both regulons were activated in the pldA mutants. The overexpression of the envelope lipoprotein, NlpE, which induces the Cpx regulon, was also found to suppress the temperature-sensitive phenotype ofdegP mutants but did not prevent the degradation of phospholipids. These results suggest that the absence of OMPLA corrects the degP temperature-sensitive phenotype by inducing the Cpx and ςE regulons rather than by inactivating the phospholipase per se.

scholarly journals Topology of the outer membrane phospholipase A of Salmonella typhimurium.

1997 ◽  
Vol 179 (11) ◽  
pp. 3443-3450 ◽  
Author(s):  
K B Merck ◽  
H de Cock ◽  
H M Verheij ◽  
J Tommassen
Keyword(s):  
Salmonella Typhimurium ◽  
Outer Membrane ◽  
Phospholipase A ◽  
Outer Membrane Phospholipase A

Substrate interferes with dimerisation of outer membrane phospholipase A

FEBS Letters ◽  
2002 ◽  
Vol 516 (1-3) ◽  
pp. 31-34 ◽  
Author(s):  
Roelie L Kingma ◽  
Maarten R Egmond
Keyword(s):  
Outer Membrane ◽  
Phospholipase A ◽  
Outer Membrane Phospholipase A

Kinetic characterization of Escherichia coli outer membrane phospholipase A using mixed detergent-lipid micelles

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1139-1147 ◽  
Author(s):  
Anton J. G. Horrevoets ◽  
Tilman M. Hackeng ◽  
Hubertus M. Verheij ◽  
Ruud Dijkman ◽  
Gerard H. De Haas
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Phospholipase A ◽  
Kinetic Characterization ◽  
Outer Membrane Phospholipase A ◽  
Lipid Micelles
Sign in / Sign up

Export Citation Format

Share Document