Calibration of Trapping Force on Cell-Size Objects From Ultrahigh-Frequency Single-Beam Acoustic Tweezer

Author(s):  
Hae Gyun Lim ◽  
Ying Li ◽  
Ming-Yi Lin ◽  
Changhan Yoon ◽  
Changyang Lee ◽  
...  
2017 ◽  
Vol 729 ◽  
pp. 556-562 ◽  
Author(s):  
Chunlong Fei ◽  
Hsiu-Sheng Hsu ◽  
Arash Vafanejad ◽  
Ying Li ◽  
Pengfei Lin ◽  
...  

2016 ◽  
Vol 109 (17) ◽  
pp. 173509 ◽  
Author(s):  
Chunlong Fei ◽  
Ying Li ◽  
Benpeng Zhu ◽  
Chi Tat Chiu ◽  
Zeyu Chen ◽  
...  

2017 ◽  
Vol 114 (11) ◽  
pp. 2637-2647 ◽  
Author(s):  
Xiaoyang Chen ◽  
Kwok Ho Lam ◽  
Ruimin Chen ◽  
Zeyu Chen ◽  
Ping Yu ◽  
...  

2020 ◽  
Vol 117 (27) ◽  
pp. 15490-15496 ◽  
Author(s):  
Diego Baresch ◽  
Valeria Garbin

Contactless manipulation of microparticles using acoustic waves holds promise for applications ranging from cell sorting to three-dimensional (3D) printing and tissue engineering. However, the unique potential of acoustic trapping to be applied in biomedical settings remains largely untapped. In particular, the main advantage of acoustic trapping over optical trapping, namely the ability of sound to propagate through thick and opaque media, has not yet been exploited in full. Here we demonstrate experimentally the use of the recently developed technique of single-beam acoustical tweezers to trap microbubbles, an important class of biomedically relevant microparticles. We show that the region of vanishing pressure of a propagating vortex beam can confine a microbubble by forcing low-amplitude, nonspherical, shape oscillations, enabling its full 3D positioning. Our interpretation is validated by the absolute calibration of the acoustic trapping force and the direct spatial mapping of isolated bubble echos, for which both find excellent agreement with our theoretical model. Furthermore, we prove the stability of the trap through centimeter-thick layers of bio-mimicking, elastic materials. Finally, we demonstrate the simultaneous trapping of nanoparticle-loaded microbubbles and activation with an independent acoustic field to trigger the release of the nanoparticles. Overall, using exclusively acoustic powering to position and actuate microbubbles paves the way toward controlled delivery of drug payloads in confined, hard-to-reach locations, with potential in vivo applications.


2020 ◽  
Vol 64 (2) ◽  
pp. 383-396
Author(s):  
Lara K. Krüger ◽  
Phong T. Tran

Abstract The mitotic spindle robustly scales with cell size in a plethora of different organisms. During development and throughout evolution, the spindle adjusts to cell size in metazoans and yeast in order to ensure faithful chromosome separation. Spindle adjustment to cell size occurs by the scaling of spindle length, spindle shape and the velocity of spindle assembly and elongation. Different mechanisms, depending on spindle structure and organism, account for these scaling relationships. The limited availability of critical spindle components, protein gradients, sequestration of spindle components, or post-translational modification and differential expression levels have been implicated in the regulation of spindle length and the spindle assembly/elongation velocity in a cell size-dependent manner. In this review, we will discuss the phenomenon and mechanisms of spindle length, spindle shape and spindle elongation velocity scaling with cell size.


2019 ◽  
Vol 83 (3) ◽  
pp. 295-308
Author(s):  
MG Weinbauer ◽  
S Suominen ◽  
J Jezbera ◽  
ME Kerros ◽  
S Marro ◽  
...  

2020 ◽  
pp. 9-12
Author(s):  
Viktor E. Lyubimov ◽  

Health of dairy cows ensures human health, so it is important that dairy products do not contain antibiotics that are used to treat any inflammation, including mastitis. In Russia at present, the problem of mastitis in cows exists with both attached and loose housing of cows. Mastitis of dairy cows are the great problem in milking husbandry. Losses from mastitis in milk yield can reach 15-20%. The main reasons for the occurrence of nonspecific mastitis of cows are the shortcomings of the working components of the milking machine: the degree of deterioration of the nipple rubber and the violation of machine milking technology (the reduction in pre-milking time and vacuum fluctuations account for 70% of all causes). Treatment of mastitis with antibiotics is effective, but not environmentally friendly and unacceptable. For the treatment of inflammation of the udder, it is necessary to use more environmentally friendly methods of treatment, one of which is the exposure to ultra-high frequency electromagnetic field tested by medicine. Use of three types of medical-mobile milking machines with the same method of exposure to ultrahigh-frequency electromagnetic fields on cow's nipples through electrodes in milking cups: LPDA-1-UHF, LPDA-2-UHF and LPDA-UHF-30 M, is described in the article. Author proved that cows with subclinical forms of mastitis recovered faster during milking with exposure to the ultrahigh frequency than when treated by antibiotics, and milking with the ultrahigh frequency device helps to recover 82% of the affected quarters with clinical forms and 100% of cows with subclinical forms of mastitis or with udder irritation. The high efficiency of the method of exposure to electromagnetic fields of the ultrahigh frequency during machine milking by means of medical-mobile milking machines LPDA-UHF for the prevention and treatment of mastitis of cows was shown.


2008 ◽  
Author(s):  
Yuankun Lin ◽  
Ahmad Harb ◽  
Daniel Rodriguez ◽  
Karen Lozano ◽  
Di Xu ◽  
...  

Author(s):  
Zsuzsanna Márton ◽  
Bianka Csitári ◽  
Tamas Felfoldi ◽  
Anna J Szekely ◽  
Attila Szabo

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