Polymer Nanoparticles Enhance Irreversible Electroporation In Vitro

2022 ◽  
pp. 1-1
Author(s):  
Ross Aaron Petrella ◽  
Shani Leah Levit ◽  
Christopher C. Fesmire ◽  
Christina Tang ◽  
Michael B. Sano
Keyword(s):  
Irreversible Electroporation ◽  
Polymer Nanoparticles

scholarly journals In Vitro and In Vivo Imaging of Peptide-Encapsulated Polymer Nanoparticles for Cancer Biomarker Activated Drug Delivery

2013 ◽  
Vol 12 (4) ◽  
pp. 304-310 ◽  
Author(s):  
Gulsim K. Kulsharova ◽  
Matthew B. Lee ◽  
Felice Cheng ◽  
Munima Haque ◽  
Hyungsoo Choi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
In Vivo Imaging ◽  
Cancer Biomarker ◽  
Polymer Nanoparticles

scholarly journals Fluorescent Polymer Nanoparticles for Cell Barcoding In Vitro and In Vivo

Small ◽  
2017 ◽  
Vol 13 (38) ◽  
pp. 1701582 ◽  
Author(s):  
Bohdan Andreiuk ◽  
Andreas Reisch ◽  
Marion Lindecker ◽  
Gautier Follain ◽  
Nadine Peyriéras ◽  
...  
Keyword(s):  
Polymer Nanoparticles ◽  
Fluorescent Polymer

scholarly journals A Three-Dimensional In Vitro Tumor Platform for Modeling Therapeutic Irreversible Electroporation

2012 ◽  
Vol 103 (9) ◽  
pp. 2033-2042 ◽  
Author(s):  
Christopher B. Arena ◽  
Christopher S. Szot ◽  
Paulo A. Garcia ◽  
Marissa Nichole Rylander ◽  
Rafael V. Davalos
Keyword(s):  
Irreversible Electroporation ◽  
Three Dimensional

In Vitro Efficacy of Paclitaxel-Loaded Dual-Responsive Shell Cross-Linked Polymer Nanoparticles Having Orthogonally Degradable Disulfide Cross-Linked Corona and Polyester Core Domains

2013 ◽  
Vol 10 (3) ◽  
pp. 1092-1099 ◽  
Author(s):  
Sandani Samarajeewa ◽  
Ritu Shrestha ◽  
Mahmoud Elsabahy ◽  
Amolkumar Karwa ◽  
Ang Li ◽  
...  
Keyword(s):  
Polymer Nanoparticles ◽  
Dual Responsive

scholarly journals Photoactivable Ruthenium-Based Coordination Polymer Nanoparticles for Light-Induced Chemotherapy

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3089
Author(s):  
Junda Zhang ◽  
Vadde Ramu ◽  
Xue-Quan Zhou ◽  
Carolina Frias ◽  
Daniel Ruiz-Molina ◽  
...  
Keyword(s):  
Coordination Polymer ◽  
High Performance ◽  
Green Light ◽  
Fold Increase ◽  
Polymer Nanoparticles ◽  
Light Irradiation ◽  
Bridging Ligands ◽  
Monomeric Complex ◽  
First Time

Green light photoactive Ru-based coordination polymer nanoparticles (CPNs), with chemical formula [[Ru(biqbpy)]1.5(bis)](PF6)3 (biqbpy = 6,6′-bis[N-(isoquinolyl)-1-amino]-2,2′-bipyridine; bis = bis(imidazol-1-yl)-hexane), were obtained through polymerization of the trans-[Ru(biqbpy)(dmso)Cl]Cl complex (Complex 1) and bis bridging ligands. The as-synthesized CPNs (50 ± 12 nm diameter) showed high colloidal and chemical stability in physiological solutions. The axial bis(imidazole) ligands coordinated to the ruthenium center were photosubstituted by water upon light irradiation in aqueous medium to generate the aqueous substituted and active ruthenium complexes. The UV-Vis spectral variations observed for the suspension upon irradiation corroborated the photoactivation of the CPNs, while High Performance Liquid Chromatography (HPLC) of irradiated particles in physiological media allowed for the first time precisely quantifying the amount of photoreleased complex from the polymeric material. In vitro studies with A431 and A549 cancer cell lines revealed an 11-fold increased uptake for the nanoparticles compared to the monomeric complex [Ru(biqbpy)(N-methylimidazole)2](PF6)2 (Complex 2). After irradiation (520 nm, 39.3 J/cm2), the CPNs yielded up to a two-fold increase in cytotoxicity compared to the same CPNs kept in the dark, indicating a selective effect by light irradiation. Meanwhile, the absence of 1O2 production from both nanostructured and monomeric prodrugs concluded that light-induced cell death is not caused by a photodynamic effect but rather by photoactivated chemotherapy.

scholarly journals Electroporation-Based Treatments in Urology

Cancers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2208 ◽  
Author(s):  
Aleksander Kiełbik ◽  
Wojciech Szlasa ◽  
Jolanta Saczko ◽  
Julita Kulbacka
Keyword(s):  
Cell Membrane ◽  
Irreversible Electroporation ◽  
Underlying Mechanism ◽  
Extracellular Proteins ◽  
Dna Transfection ◽  
Membrane Phospholipids ◽  
Cancer Antigens ◽  
Urological Cancers

The observation that an application of a pulsed electric field (PEF) resulted in an increased permeability of the cell membrane has led to the discovery of the phenomenon called electroporation (EP). Depending on the parameters of the electric current and cell features, electroporation can be either reversible or irreversible. The irreversible electroporation (IRE) found its use in urology as a non-thermal ablative method of prostate and renal cancer. As its mechanism is based on the permeabilization of cell membrane phospholipids, IRE (as well as other treatments based on EP) provides selectivity sparing extracellular proteins and matrix. Reversible EP enables the transfer of genes, drugs, and small exogenous proteins. In clinical practice, reversible EP can locally increase the uptake of cytotoxic drugs such as cisplatin and bleomycin. This approach is known as electrochemotherapy (ECT). Few in vivo and in vitro trials of ECT have been performed on urological cancers. EP provides the possibility of transmission of genes across the cell membrane. As the protocols of gene electrotransfer (GET) over the last few years have improved, EP has become a well-known technique for non-viral cell transfection. GET involves DNA transfection directly to the cancer or the host skin and muscle tissue. Among urological cancers, the GET of several plasmids encoding prostate cancer antigens has been investigated in clinical trials. This review brings into discussion the underlying mechanism of EP and an overview of the latest progress and development perspectives of EP-based treatments in urology.

scholarly journals Establishing Irreversible Electroporation Electric Field Potential Threshold in A Suspension In Vitro Model for Cardiac and Neuronal Cells

2021 ◽  
Vol 10 (22) ◽  
pp. 5443
Author(s):  
Sahar Avazzadeh ◽  
Barry O’Brien ◽  
Ken Coffey ◽  
Martin O’Halloran ◽  
David Keane ◽  
...  
Keyword(s):  
Cell Death ◽  
Electric Field ◽  
Cell Viability ◽  
Cardiac Fibroblasts ◽  
Irreversible Electroporation ◽  
Field Potential ◽  
Optimal Threshold ◽  
Delayed Cell Death ◽  
Significant Difference

Aims: Irreversible electroporation is an ablation technique being adapted for the treatment of atrial fibrillation. Currently, there are many differences reported in the in vitro and pre-clinical literature for the effective voltage threshold for ablation. The aim of this study is a direct comparison of different cell types within the cardiovascular system and identification of optimal voltage thresholds for selective cell ablation. Methods: Monophasic voltage pulses were delivered in a cuvette suspension model. Cell viability and live–dead measurements of three different neuronal lines, cardiomyocytes, and cardiac fibroblasts were assessed under different voltage conditions. The immediate effects of voltage and the evolution of cell death was measured at three different time points post ablation. Results: All neuronal and atrial cardiomyocyte lines showed cell viability of less than 20% at an electric field of 1000 V/cm when at least 30 pulses were applied with no significant difference amongst them. In contrast, cardiac fibroblasts showed an optimal threshold at 1250 V/cm with a minimum of 50 pulses. Cell death overtime showed an immediate or delayed cell death with a proportion of cell membranes re-sealing after three hours but no significant difference was observed between treatments after 24 h. Conclusions: The present data suggest that understanding the optimal threshold of irreversible electroporation is vital for achieving a safe ablation modality without any side-effect in nearby cells. Moreover, the evolution of cell death post electroporation is key to obtaining a full understanding of the effects of IRE and selection of an optimal ablation threshold.

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