Automation and optimization of multi-pulse laser zona drilling of mouse embryos during embryo biopsy

2016 ◽  
pp. 1-1 ◽  
Author(s):  
Christopher Yee Wong ◽  
James K. Mills
Keyword(s):  
Pulse Laser ◽  
Mouse Embryos ◽  
Embryo Biopsy ◽  
Zona Drilling

Developmental competence of mouse embryos following zona drilling using a non-contact HO:Yag laser system

1995 ◽  
Vol 43 (1) ◽  
pp. 315
Author(s):  
M.C. Schiewe ◽  
J.Y. Neev ◽  
M.L. Hazeleger ◽  
Y. Tadir ◽  
M.W. Berns ◽  
...  
Keyword(s):  
Laser System ◽  
Mouse Embryos ◽  
Developmental Competence ◽  
Ho:Yag Laser ◽  
Zona Drilling

Developmental competence of mouse embryos following zona drilling using a non-contact holmium: yttrium scandian gallium garnet (Ho: YSGG) laser system

1995 ◽  
Vol 10 (7) ◽  
pp. 1821-1824 ◽  
Author(s):  
Mitchel C. Schiewe ◽  
Joseph Neev ◽  
Nancy L. Hazeleger ◽  
Jose P. Balmaceda ◽  
Michael W. Berns ◽  
...  
Keyword(s):  
Laser System ◽  
Mouse Embryos ◽  
Developmental Competence ◽  
Ysgg Laser ◽  
Zona Drilling

Comparison of Computer Assisted Infrared Laser with Acid Tyrode Solution in zona drilling for embryo biopsy

2004 ◽  
Vol 82 ◽  
pp. S250-S251
Author(s):  
M. Li ◽  
C. Marin Deugarte ◽  
M. Surrey ◽  
H. Danzer ◽  
A. Decherney ◽  
...  
Keyword(s):  
Infrared Laser ◽  
Embryo Biopsy ◽  
Computer Assisted ◽  
Tyrode Solution ◽  
Zona Drilling

A simplified biopsy method for precompacted mouse embryos: A technical report

2002 ◽  
Vol 50 (4) ◽  
pp. 469-479 ◽  
Author(s):  
S. Bodó ◽  
L. Laczkó ◽  
Gabriella Horváth ◽  
Keyword(s):  
Genetic Diagnosis ◽  
In Utero ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Embryo Biopsy ◽  
Control Groups ◽  
Technical Report ◽  
Significant Difference ◽  
Biopsy Method

This article presents a new, simple and rapid embryo biopsy method. The blastomere for genetic analysis can be separated from a precompacted mouse embryo after a partial zona digestion with the use of a holding pipette. For the micromanipulation only two microcapillaries and micromanipulators are needed. The development of the biopsied embryos was studied during in vitro culture and in utero following embryo transfer. There was no significant difference between the treated and the control groups in the ratio of embryos that developed to the blastocyst stage, although the biopsied embryos were delayed in their development because they contained significantly fewer cells compared to the control ones at the same stage. Although there was no difference in the ratio of implantation, the development of the biopsied embryos in utero was also delayed 12-24 hours on the 9th day of pregnancy. No difference in development was visible from the 13th day of pregnancy. Statistically, no differences were found in the developmental ratio (number of developed fetuses/transferred embryos) of the control and treated embryos during gastrulation (9th day of pregnancy), at the beginning of organogenesis (13th day of pregnancy) and before birth (19th day of pregnancy). The embryo biopsy method presented here can be a new and useful tool for preimplantation genetic diagnosis.

Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos

2005 ◽  
Vol 84 (5) ◽  
pp. 1547-1550 ◽  
Author(s):  
Cristina Hartshorn ◽  
Aleksandra Anshelevich ◽  
Lawrence J. Wangh
Keyword(s):  
Mouse Embryos ◽  
Zona Drilling

scholarly journals Sex diagnosis of ovine and bovine embryos by enzymatic amplification and digestion of DNA from the ZFY/ZFX locus

1992 ◽  
Vol 1 (2) ◽  
pp. 233-238
Author(s):  
Peter Bredbacka ◽  
Jaana Peippo
Keyword(s):  
Sex Determination ◽  
Y Chromosome ◽  
Alkaline Treatment ◽  
Mouse Embryos ◽  
Restriction Endonucleases ◽  
Embryo Biopsy ◽  
Bovine Embryos ◽  
Enzymatic Amplification ◽  
Sex Diagnosis ◽  
Blind Trials

A PCR-based sex determination assay for sheep and cattle embryos was developed using mouse embryos for optimizing the protocol. Samples were lysed either enzymatically or by alkaline treatment followed by enzymatic amplification of DNA from the ZFY/ZFX locus. Sex diagnosis could be done after the digestion of the amplified product by restriction endonucleases. Ovine and bovine embryos could be sexed from biopsies as small as 1-4 cells. Some embryos were split into 2-4 sections, which were amplified separately. Blind trials with such samples demonstrated that the method was highly accurate, even when embryo biopsy was done under farm conditions. The protocol involves an in-built control. This eliminates the need for autosomal control primers, which often inhibit the amplification of the Y-chromosome-specific DNA, especially when a small amount of template is used.

scholarly journals A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

1997 ◽  
Vol 14 (3) ◽  
pp. 157-161 ◽  
Author(s):  
Shee-Uan Chen ◽  
Hong-Nerng Ho ◽  
Hsin-Fu Chen ◽  
Kuang-Han Chao ◽  
Su-Cheng Huang ◽  
...  
Keyword(s):  
Embryo Biopsy ◽  
Zona Drilling
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