scholarly journals A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

1997 ◽  
Vol 14 (3) ◽  
pp. 157-161 ◽  
Author(s):  
Shee-Uan Chen ◽  
Hong-Nerng Ho ◽  
Hsin-Fu Chen ◽  
Kuang-Han Chao ◽  
Su-Cheng Huang ◽  
...  
Keyword(s):  
2004 ◽  
Vol 82 ◽  
pp. S250-S251
Author(s):  
M. Li ◽  
C. Marin Deugarte ◽  
M. Surrey ◽  
H. Danzer ◽  
A. Decherney ◽  
...  

2005 ◽  
Vol 84 ◽  
pp. S346-S347
Author(s):  
S. Geber ◽  
L. Sales ◽  
R. Bossi ◽  
P. Souza ◽  
M.A. Sampaio

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
M. Cenariu ◽  
E. Pall ◽  
C. Cernea ◽  
I. Groza

The purpose of this research was to evaluate three embryo biopsy techniques used for preimplantation genetic diagnosis (PGD) in cattle and to recommend the least invasive one for current use, especially when PGD is followed by embryo cryopreservation. Three hundred bovine embryos were biopsied by either one of the needle, aspiration or microblade method, and then checked for viability by freezing/thawing and transplantation to recipient cows. The number of pregnancies obtained after the transfer of biopsied frozen/thawed embryos was assessed 30 days later using ultrasounds. The results were significantly different between the three biopsy methods: the pregnancy rate was of 57% in cows that received embryos biopsied by needle, 43% in cows that received embryos biopsied by aspiration, and 31% in cows that received embryos biopsied by microblade. Choosing an adequate biopsy method is therefore of great importance in embryos that will undergo subsequent cryopreservation, as it significantly influences their viability after thawing.


1989 ◽  
Vol 40 (5) ◽  
pp. 953-960 ◽  
Author(s):  
B. C. Vanderhyden ◽  
K. J. Mc Laughlin ◽  
J. M. Rutledege ◽  
D. T. Armstrong
Keyword(s):  

1990 ◽  
Vol 42 (5-6) ◽  
pp. 869-876 ◽  
Author(s):  
Jon W. Gordon ◽  
Ingoo Gang
Keyword(s):  

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