Investigation of Low-Voltage Pulse Parameters on Electroporation and Electrical Lysis Using a Microfluidic Device With Interdigitated Electrodes

2014 ◽  
Vol 61 (3) ◽  
pp. 871-882 ◽  
Author(s):  
Bashir I. Morshed ◽  
Maitham Shams ◽  
Tofy Mussivand
Keyword(s):  
Microfluidic Device ◽  
Voltage Pulse ◽  
Low Voltage ◽  
Interdigitated Electrodes ◽  
Pulse Parameters ◽  
Electrical Lysis

scholarly journals Continuous electrical lysis of cancer cells in a microfluidic device with passivated interdigitated electrodes

2020 ◽  
Vol 14 (6) ◽  
pp. 064101
Author(s):  
K. Pandian ◽  
M. Ajanth Praveen ◽  
S. Z. Hoque ◽  
A. Sudeepthi ◽  
A. K. Sen
Keyword(s):  
Cancer Cells ◽  
Microfluidic Device ◽  
Interdigitated Electrodes ◽  
Electrical Lysis

Fundamental Study of Cannabidiol Effect on MCF-7 with Low Voltage Pulse Electric Field

2021 ◽  
Author(s):  
Nur Adilah Abd Rahman ◽  
Muhammad Mahadi Abdul Jamil ◽  
Mohamad Nazib Adon ◽  
Ahmad Basri Zainal ◽  
Farideh Javid ◽  
...  
Keyword(s):  
Electric Field ◽  
Voltage Pulse ◽  
Low Voltage ◽  
Pulse Electric Field ◽  
Fundamental Study ◽  
Pulse Electric ◽  
Mcf 7

scholarly journals Electrophoretic Concentration and Electrical Lysis of Bacteria in a Microfluidic Device Using a Nanoporous Membrane

Micromachines ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 45 ◽  
Author(s):  
Md. Islam ◽  
Ali Shahid ◽  
Kacper Kuryllo ◽  
Yingfu Li ◽  
M. Deen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nanoporous Membrane ◽  
Electrophoretic Concentration ◽  
Electrical Lysis

Calcium influx determines the muscular response to electrotransfer

2012 ◽  
Vol 302 (4) ◽  
pp. R446-R453 ◽  
Author(s):  
Pernille Hojman ◽  
Camilla Brolin ◽  
Hanne Gissel
Keyword(s):  
Cell Membrane ◽  
High Voltage ◽  
Voltage Pulse ◽  
Low Voltage ◽  
Calcium Influx ◽  
High Voltage Pulse ◽  
Electric Pulses ◽  
Tnf Α ◽  
Cell Membrane Permeabilization ◽  
The Times

Cell membrane permeabilization by electric pulses (electropermeabilization), results in free exchange of ions across the cell membrane. The role of electrotransfer-mediated Ca2+-influx on muscle signaling pathways involved in degeneration (β-actin and MurF), inflammation (IL-6 and TNF-α), and regeneration (MyoD1, myogenin, and Myf5) was investigated, using pulse parameters of both electrochemotherapy (8 HV) and DNA delivery (HVLV). Three pulsing conditions were used: 8 high-voltage pulses (8 HV), resulting in large permeabilization and ion flux, and a combination of one high-voltage pulse and one low-voltage pulse (HVLV), either alone or in combination with injection of DNA. Mice and rats were anesthetized before pulsing. At the times given, animals were killed, and intact tibialis cranialis muscles were excised for analysis. Uptake of Ca2+ was assessed using 45Ca as a tracer. Using gene expression analyses and histology, we showed a clear association between Ca2+ influx and muscular response. Moderate Ca2+ influx induced by HVLV pulses results in activation of pathways involved in immediate repair and hypertrophy. This response could be attenuated by intramuscular injection of EGTA reducing Ca2+ influx. Larger Ca2+ influx as induced by 8-HV pulses leads to muscle damage and muscle fiber regeneration through recruitment of satellite cells. The extent of Ca2+ influx determines the muscular response to electrotransfer and, thus, the success of a given application. In the case of electrochemotherapy, in which the objective is cell death, a large influx of Ca2+ may be beneficial, whereas for DNA electrotransfer, muscle recovery should occur without myofiber loss to ensure preservation of plasmid DNA.

scholarly journals A Dielectrophoresis Microfluidic Device for Trapping Bioparticles at Low Voltage and Frequency

2016 ◽  
Vol 41 (1) ◽  
pp. 60-65
Author(s):  
Jin-Tae Jeong ◽  
Hyun-Min Shin ◽  
Duwoon Kim ◽  
Kyeong-Hwan Lee
Keyword(s):  
Microfluidic Device ◽  
Low Voltage

Implementation of a High Voltage Pulse Modulator with Low Voltage Ripple and Fast Switching Characteristics

2012 ◽  
Vol 9 (1) ◽  
pp. 466-471
Author(s):  
Hung-Liang Cheng ◽  
Chien-Hsuan Chang ◽  
Chun-An Cheng ◽  
Chin-Wen Chuang
Keyword(s):  
High Voltage ◽  
Voltage Pulse ◽  
Low Voltage ◽  
High Voltage Pulse ◽  
Fast Switching ◽  
Voltage Ripple ◽  
Pulse Modulator ◽  
Switching Characteristics

Design of a Two Levels Self-Integrating Capacitive Divider

2014 ◽  
Vol 599-601 ◽  
pp. 882-886
Author(s):  
Bao Feng Cao ◽  
Jiang Bing Fan ◽  
Li Jun Song ◽  
Xue Qin Zhang ◽  
Xin Li ◽  
...  
Keyword(s):  
High Voltage ◽  
Voltage Pulse ◽  
Low Voltage ◽  
Voltage Divider ◽  
High Voltage Pulse ◽  
Pulse Source ◽  
Voltage Level ◽  
Capacitive Divider ◽  
Capacitive Voltage ◽  
Capacitive Voltage Divider

A ims . A kind of two levels self-integrating capacitive voltage divider is designed to online measure high voltage nanosecond pulse. Methods. The circuit principle of capacitive divider is analyzed, and the theoretical voltage ratio is calculated. The calibration is carried out by using a low voltage pulse source. The divider with a primary voltage divide ratio 601 has been tested to frequency response exceed 1GHz. The second voltage divide ratio can be set flexibly using different ratio attenuators according to the different voltage level of the source. Result. Finally, this divider is used in measuring 600kV high voltage pulse source, and the experimental results show that the present coaxial capacitive divider is reliable and satisfactory for the measuring requirement in time domain and frequency domain.

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