scholarly journals Continuous electrical lysis of cancer cells in a microfluidic device with passivated interdigitated electrodes

2020 ◽  
Vol 14 (6) ◽  
pp. 064101
Author(s):  
K. Pandian ◽  
M. Ajanth Praveen ◽  
S. Z. Hoque ◽  
A. Sudeepthi ◽  
A. K. Sen
Keyword(s):  
Cancer Cells ◽  
Microfluidic Device ◽  
Interdigitated Electrodes ◽  
Electrical Lysis

A nanostructured microfluidic device for plasmonic-assisted electrochemical detection of hydrogen peroxide released from cancer cells

Nanoscale ◽  
2021 ◽  
Author(s):  
Carolina del Real Mata ◽  
Roozbeh Siavash Moakhar ◽  
Sayed Iman Isaac Hosseini ◽  
Mahsa Jalali ◽  
Sara Mahshid
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Real Time ◽  
Electrochemical Detection ◽  
Microfluidic Device ◽  
Cancer Biomarker ◽  
Cancer Diagnostics ◽  
Liquid Biopsies ◽  
Non Invasive

Non-invasive liquid biopsies offer hope for a rapid, risk-free, real-time glimpse into cancer diagnostics. Recently, hydrogen peroxide (H2O2) is identified as a cancer biomarker due to continued release from cancer...

scholarly journals Electrophoretic Concentration and Electrical Lysis of Bacteria in a Microfluidic Device Using a Nanoporous Membrane

Micromachines ◽  
2017 ◽  
Vol 8 (2) ◽  
pp. 45 ◽  
Author(s):  
Md. Islam ◽  
Ali Shahid ◽  
Kacper Kuryllo ◽  
Yingfu Li ◽  
M. Deen ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Nanoporous Membrane ◽  
Electrophoretic Concentration ◽  
Electrical Lysis

Inertial Migration of Cancer Cells in a Microfluidic Device

2012 ◽  
pp. 15-27
Author(s):  
Tatsuya Tanaka ◽  
Takuji Ishikawa ◽  
Keiko Numayama-Tsuruta ◽  
Yohsuke Imai ◽  
Hironori Ueno ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Device ◽  
Inertial Migration

scholarly journals Effect of Varying Expression of EpCAM on the Efficiency of CTCs Detection by SERS-Based Immunomagnetic Optofluidic Device

Cancers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3315
Author(s):  
Marta Czaplicka ◽  
Krzysztof Niciński ◽  
Ariadna Nowicka ◽  
Tomasz Szymborski ◽  
Izabela Chmielewska ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Device ◽  
Surface Enhanced Raman Spectroscopy ◽  
Prostate Adenocarcinoma ◽  
Immune Recognition ◽  
Blood Samples ◽  
Magnetic Nanospheres ◽  
Western Blot Method ◽  
Isolation And Characterization ◽  
Adenocarcinoma Cells

The circulating tumor cells (CTCs) isolation and characterization has a great potential for non-invasive biopsy. In the present research, the surface–enhanced Raman spectroscopy (SERS)-based assay utilizing magnetic nanoparticles and solid SERS-active support integrated in the external field assisted microfluidic device was designed for efficient isolation of CTCs from blood samples. Magnetic nanospheres (Fe2O3) were coated with SERS-active metal and then modified with p-mercaptobenzoic acid (p-MBA) which works simultaneously as a Raman reporter and linker to an antiepithelial-cell-adhesion-molecule (anti-EpCAM) antibodies. The newly developed laser-induced SERS-active silicon substrate with a very strong enhancement factor (up to 108) and high stability and reproducibility provide the additional extra-enhancement in the sandwich plasmonic configuration of immune assay which finally leads to increase the efficiency of detection. The sensitive immune recognition of cancer cells is assisted by the introducing of the controllable external magnetic field into the microfluidic chip. Moreover, the integration of the SERS-active platform and p-MBA-labeled immuno-Ag@Fe2O3 nanostructures with microfluidic device offers less sample and analytes demand, precise operation, increase reproducibly of spectral responses, and enables miniaturization and portability of the presented approach. In this work, we have also investigated the effect of varying expression of the EpCAM established by the Western Blot method supported by immunochemistry on the efficiency of CTCs’ detection with the developed SERS method. We used four target cancer cell lines with relatively high (human metastatic prostate adenocarcinoma cells (LNCaP)), medium (human metastatic prostate adenocarcinoma cells (LNCaP)), weak (human metastatic prostate adenocarcinoma cells (LNCaP)), and no EpCAM expressions (cervical cancer cells (HeLa)) to estimate the limits of detection based on constructed calibration curves. Finally, blood samples from lung cancer patients were used to validate the efficiency of the developed method in clinical trials.

21am2-E4 Microfluidic device for one-to-one electrofusion and selective transfer of cytoplasmic contents from cancer cells to dendritic cells

2014 ◽  
Vol 2014.6 (0) ◽  
pp. _21am2-E4--_21am2-E4-
Author(s):  
Yutaro Itagaki ◽  
Kennedy Omondi Okeyo ◽  
Osamu Kurosawa ◽  
Miwako Narita ◽  
Hidehiro Oana ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cancer Cells ◽  
Microfluidic Device ◽  
Selective Transfer ◽  
One To One

scholarly journals Microfluidic Techniques for Single-Cell Protein Expression Analysis

2006 ◽  
Vol 52 (6) ◽  
pp. 1080-1088 ◽  
Author(s):  
Ethan Fitzpatrick ◽  
Sterling McBride ◽  
Jonathan Yavelow ◽  
Saltanat Najmi ◽  
Peter Zanzucchi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Protein Expression ◽  
Cancer Cells ◽  
Microfluidic Device ◽  
Single Cells ◽  
Surface Protein ◽  
Surface Proteins ◽  
Cell Protein ◽  
Surface Protein Expression ◽  
Mcf 7

Abstract Background: The analysis of single cells obtained from needle aspirates of tumors is constrained by the need for processing. To this end, we investigated two microfluidic approaches to measure the expression of surface proteins in single cancer cells or in small populations (<50 cells). Methods: One approach involved indirect fluorescence labeling of cell-surface proteins and channeling of cells in a microfluidic device past a fluorescence detector for signal quantification and analysis. A second approach channeled cells in a microfluidic device over detection zones coated with ligands to surface proteins and measured rates of passage and of retardation based on transient interactions between surface proteins and ligands. Results: The fluorescence device detected expression of integrin α5 induced by basic fibroblast growth factor (FGF-2) treatment in MCF-7 cells and that of Her-2/neu in SK-BR-3 cells compared with controls. Experiments measuring passage retardation showed significant differences in passage rates between FGF-2–treated and untreated MCF-7 cells over reaction regions coated with fibronectin and antibody to integrin α5β1 compared with control regions. Blocking peptides reversed the retardation, demonstrating specificity. Conclusions: Immunofluorescence detection in a microfluidic channel demonstrates the potential for assaying surface protein expression in a few individual cells and will permit the development of future iterations not requiring cell handling. The flow retardation device represents the first application of this technology for assessing cell-surface protein expression in cancer cells and may provide a way for analyzing expression profiles of single cells without preanalytical manipulation.

Specific Binding of Cancer Cells Using a Microchamber Array Functionalized With Antibodies

2009 ◽  
Author(s):  
Xiangjun Zheng ◽  
Siu Lun Cheung ◽  
Lian Wang ◽  
Joyce A. Schroeder ◽  
Ronald L. Heimark ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Microfluidic Device ◽  
Specific Binding ◽  
Metastatic Cancer ◽  
Cell Cancer ◽  
Membrane Receptors ◽  
Oxide Surface ◽  
Cell Capture ◽  
Cell Receptors ◽  
Suspended Cells

Specific binding of target suspended metastatic cancer cells to an antibody-functionalized surface utilizing a microfluidic device has experimentally been investigated under various conditions. The microfluidic devices, fabricated in silicon using DRIE process, consisted of a 5×5 micochamber array; each 1mm×1mm in area, and 50μm in depth. The oxide surface of the microchammber array was functionalized with various antibodies immobilized on a protein G layer. The microfluidic device design allows accurate counting of cells loaded into each microchamber and, thus, enabling a reliable counting of cells captured from homogeneous suspensions. The effects of cell suspension concentration, incubation times and ambient temperature on cell capture efficiency have been examined. Furthermore, to evaluate the specificity of the cell-surface interaction, several cell cancer types expressing different membrane receptors have been incubated on surfaces functionalized with various counter receptors. Specific binding of up to 100% of the suspended cells is observed when using surfaces functionalized with counter receptors matching the cell receptors; otherwise, non-specific binding of less than 15% of suspended cells is obtained if the functionalized counter receptors do not match the cell receptors.

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