Dual Electroretinogram/Nitric Oxide Carbon Fiber Microelectrode for Direct Measurement of Nitric Oxide in the In Vivo Retina

2014 ◽  
Vol 61 (3) ◽  
pp. 611-619
Author(s):  
Micah J. Guthrie ◽  
Jennifer J. Kang-Mieler
Keyword(s):  
Nitric Oxide ◽  
Carbon Fiber ◽  
Direct Measurement ◽  
Carbon Fiber Microelectrode

In Vivo Monitoring of Oxygen in Rat Brain by Carbon Fiber Microelectrode Modified with Antifouling Nanoporous Membrane

2019 ◽  
Vol 91 (5) ◽  
pp. 3645-3651 ◽  
Author(s):  
Lin Zhou ◽  
Hanfeng Hou ◽  
Huan Wei ◽  
Lina Yao ◽  
Lei Sun ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Rat Brain ◽  
Nanoporous Membrane ◽  
In Vivo Monitoring ◽  
Carbon Fiber Microelectrode

A sensitive nitric oxide microsensor based on PBPB composite film-modified carbon fiber microelectrode

2008 ◽  
Vol 133 (2) ◽  
pp. 571-576 ◽  
Author(s):  
Yanfen Peng ◽  
Chengguo Hu ◽  
Dongyun Zheng ◽  
Shengshui Hu
Keyword(s):  
Nitric Oxide ◽  
Carbon Fiber ◽  
Composite Film ◽  
Carbon Fiber Microelectrode

Ferricyanide-backfilled cylindrical carbon fiber microelectrodes for in vivo analysis with high stability and low polarized potential

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7154-7159 ◽  
Author(s):  
Peipei Zhong ◽  
Ping Yu ◽  
Kai Wang ◽  
Jie Hao ◽  
Junjie Fei ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
High Stability ◽  
Carbon Fiber Microelectrode ◽  
In Vivo Analysis ◽  
Carbon Fiber Microelectrodes

A ferricyanide-backfilled cylindrical carbon fiber microelectrode of high stability and low polarized potential was fabricated and used for in vivo analysis.

scholarly journals In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis

2012 ◽  
Vol 107 (6) ◽  
pp. 1731-1737 ◽  
Author(s):  
Natalie R. Herr ◽  
Jinwoo Park ◽  
Zoé A. McElligott ◽  
Anna M. Belle ◽  
Regina M. Carelli ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Mode Of Delivery ◽  
Simultaneous Detection ◽  
Local Application ◽  
Stria Terminalis ◽  
Pharmacological Agents ◽  
Bed Nucleus ◽  
Carbon Fiber Microelectrode ◽  
Systemic Application

Norepinephrine (NE) is an easily oxidized neurotransmitter that is found throughout the brain. Considerable evidence suggests that it plays an important role in neurocircuitry related to fear and anxiety responses. In certain subregions of the bed nucleus of the stria terminalis (BNST), NE is found in large amounts. In this work we probed differences in electrically evoked release of NE and its regulation by the norepinephrine transporter (NET) and the α2-adrenergic autoreceptor (α2-AR) in two regions of the BNST of anesthetized rats. NE was monitored in the dorsomedial BNST (dmBNST) and ventral BNST (vBNST) by fast-scan cyclic voltammetry at carbon fiber microelectrodes. Pharmacological agents were introduced either by systemic application (intraperitoneal injection) or by local application (iontophoresis). The iontophoresis barrels were attached to a carbon fiber microelectrode to allow simultaneous detection of evoked NE release and quantitation of iontophoretic delivery. Desipramine (DMI), an inhibitor of NET, increased evoked release and slowed clearance of released NE in both regions independent of the mode of delivery. However, the effects of DMI were more robust in the vBNST than in the dmBNST. Similarly, the α2-AR autoreceptor inhibitor idazoxan (IDA) enhanced NE release in both regions but to a greater extent in the vBNST by both modes of delivery. Since both local application by iontophoresis and systemic application of IDA had similar effects on NE release, our results indicate that terminal autoreceptors play a predominant role in the inhibition of subsequent release.

scholarly journals Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

10.3791/62760 ◽  
2021 ◽  
Author(s):  
Kristen N. Reikersdorfer ◽  
Andrea K. Stacy ◽  
David A. Bressler ◽  
Lauren S. Hayashi ◽  
Keith B. Hengen ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Microelectrode Arrays ◽  
Carbon Fiber Microelectrode

A new design of carbon fiber microelectrode for in vivo voltammetry using fused silica

1997 ◽  
Vol 73 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Artur H Swiergiel ◽  
Vitaly S Palamarchouk ◽  
Adrian J Dunn
Keyword(s):  
Carbon Fiber ◽  
Fused Silica ◽  
In Vivo Voltammetry ◽  
Carbon Fiber Microelectrode

In vivo measurement of tubular fluid ferrocyanide with carbon-fiber microelectrodes

1987 ◽  
Vol 252 (6) ◽  
pp. F1158-F1166
Author(s):  
L. C. Moore ◽  
C. Clausen ◽  
E. F. Bowden ◽  
A. Birzgalis
Keyword(s):  
Carbon Fiber ◽  
Proximal Tubule ◽  
Linear Response ◽  
Ion Concentration ◽  
Data Sampling ◽  
Sampling Device ◽  
Carbon Fiber Microelectrode ◽  
Carbon Fiber Microelectrodes

Techniques to construct carbon-fiber microelectrodes and to measure ferrocyanide ion concentration in single nephrons are described. The measurement involves polarizing an inert carbon-fiber microelectrode 500 mV positive with respect to a Ag-AgCl reference, while measuring the faradic current produced by the oxidation of ferrocyanide. A carbon fiber (5-7 micron diam) is heat sealed into a glass micropipette that is then sharpened, silanized, and electrochemically pretreated to minimize electrode degradation by protein. Circuit diagrams for an inexpensive voltage clamp-current monitor and a data sampling device are presented. The electrodes show a linear response to changes in ferrocyanide concentration in large and very small (20 nl) volumes in vitro. The electrodes were used in an electrochemical microassay to determine tubular fluid-to-plasma ferrocyanide concentration ratios and nephron filtration rates with proximal micropuncture samples. The results show excellent agreement with paired determinations using [3H]inulin. In vivo proximal tubule perfusion experiments show a rapid linear response to changes in tubular fluid ferrocyanide concentration. These electrodes permit rapid quantitative measurements of ferrocyanide concentration and water transport in the proximal tubule and may be useful in other biological systems.

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