Ferricyanide-backfilled cylindrical carbon fiber microelectrodes for in vivo analysis with high stability and low polarized potential

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7154-7159 ◽  
Author(s):  
Peipei Zhong ◽  
Ping Yu ◽  
Kai Wang ◽  
Jie Hao ◽  
Junjie Fei ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
High Stability ◽  
Carbon Fiber Microelectrode ◽  
In Vivo Analysis ◽  
Carbon Fiber Microelectrodes

A ferricyanide-backfilled cylindrical carbon fiber microelectrode of high stability and low polarized potential was fabricated and used for in vivo analysis.

In vivo measurement of tubular fluid ferrocyanide with carbon-fiber microelectrodes

1987 ◽  
Vol 252 (6) ◽  
pp. F1158-F1166
Author(s):  
L. C. Moore ◽  
C. Clausen ◽  
E. F. Bowden ◽  
A. Birzgalis
Keyword(s):  
Carbon Fiber ◽  
Proximal Tubule ◽  
Linear Response ◽  
Ion Concentration ◽  
Data Sampling ◽  
Sampling Device ◽  
Carbon Fiber Microelectrode ◽  
Carbon Fiber Microelectrodes

Techniques to construct carbon-fiber microelectrodes and to measure ferrocyanide ion concentration in single nephrons are described. The measurement involves polarizing an inert carbon-fiber microelectrode 500 mV positive with respect to a Ag-AgCl reference, while measuring the faradic current produced by the oxidation of ferrocyanide. A carbon fiber (5-7 micron diam) is heat sealed into a glass micropipette that is then sharpened, silanized, and electrochemically pretreated to minimize electrode degradation by protein. Circuit diagrams for an inexpensive voltage clamp-current monitor and a data sampling device are presented. The electrodes show a linear response to changes in ferrocyanide concentration in large and very small (20 nl) volumes in vitro. The electrodes were used in an electrochemical microassay to determine tubular fluid-to-plasma ferrocyanide concentration ratios and nephron filtration rates with proximal micropuncture samples. The results show excellent agreement with paired determinations using [3H]inulin. In vivo proximal tubule perfusion experiments show a rapid linear response to changes in tubular fluid ferrocyanide concentration. These electrodes permit rapid quantitative measurements of ferrocyanide concentration and water transport in the proximal tubule and may be useful in other biological systems.

In Vivo Monitoring of Oxygen in Rat Brain by Carbon Fiber Microelectrode Modified with Antifouling Nanoporous Membrane

2019 ◽  
Vol 91 (5) ◽  
pp. 3645-3651 ◽  
Author(s):  
Lin Zhou ◽  
Hanfeng Hou ◽  
Huan Wei ◽  
Lina Yao ◽  
Lei Sun ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Rat Brain ◽  
Nanoporous Membrane ◽  
In Vivo Monitoring ◽  
Carbon Fiber Microelectrode

Facile Fabrication and Characterization of Carbon Fiber Microelectrode

2011 ◽  
Vol 287-290 ◽  
pp. 1433-1436
Author(s):  
Zhan Jun Yang ◽  
Yan Yan Ren ◽  
Juan Li ◽  
Xiao Ya Hu
Keyword(s):  
Electron Microscopy ◽  
Carbon Fiber ◽  
Electrochemical Behaviors ◽  
Carbon Fiber Microelectrode ◽  
Facile Fabrication ◽  
Fabrication And Characterization ◽  
Carbon Fiber Microelectrodes ◽  
Novel Strategy ◽  
First Time

A novel strategy was proposed to fabricate carbon fiber microelectrodes (CFMEs). The resultant CFMEs were characterized using scan electron microscopy (SEM) and cyclic voltammetry (CV). Compared to the conventional method, the proposed method only needs a simple heating step for achieving CFMEs without additional pulled, sealed and back-filled procedure. The electrochemical behaviors of 2,4-Dichlorophenol (2,4-DCP) at fabricated CFMEs in pH (2.0-9.0) was for the first time studied and demonstrated a two-charge and two-proton transference process.

scholarly journals In Vivo Ambient Serotonin Measurements at Carbon-Fiber Microelectrodes

2017 ◽  
Vol 89 (18) ◽  
pp. 9703-9711 ◽  
Author(s):  
Aya Abdalla ◽  
Christopher W. Atcherley ◽  
Pavithra Pathirathna ◽  
Srimal Samaranayake ◽  
Beidi Qiang ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Carbon Fiber Microelectrodes

Carbon Nanotube-Modified Carbon Fiber Microelectrodes for In Vivo Voltammetric Measurement of Ascorbic Acid in Rat Brain

2007 ◽  
Vol 79 (17) ◽  
pp. 6559-6565 ◽  
Author(s):  
Meining Zhang ◽  
Kun Liu ◽  
Ling Xiang ◽  
Yuqing Lin ◽  
Lei Su ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Carbon Nanotube ◽  
Carbon Fiber ◽  
Rat Brain ◽  
Voltammetric Measurement ◽  
Carbon Fiber Microelectrodes

scholarly journals In vivo voltammetry monitoring of electrically evoked extracellular norepinephrine in subregions of the bed nucleus of the stria terminalis

2012 ◽  
Vol 107 (6) ◽  
pp. 1731-1737 ◽  
Author(s):  
Natalie R. Herr ◽  
Jinwoo Park ◽  
Zoé A. McElligott ◽  
Anna M. Belle ◽  
Regina M. Carelli ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Mode Of Delivery ◽  
Simultaneous Detection ◽  
Local Application ◽  
Stria Terminalis ◽  
Pharmacological Agents ◽  
Bed Nucleus ◽  
Carbon Fiber Microelectrode ◽  
Systemic Application

Norepinephrine (NE) is an easily oxidized neurotransmitter that is found throughout the brain. Considerable evidence suggests that it plays an important role in neurocircuitry related to fear and anxiety responses. In certain subregions of the bed nucleus of the stria terminalis (BNST), NE is found in large amounts. In this work we probed differences in electrically evoked release of NE and its regulation by the norepinephrine transporter (NET) and the α2-adrenergic autoreceptor (α2-AR) in two regions of the BNST of anesthetized rats. NE was monitored in the dorsomedial BNST (dmBNST) and ventral BNST (vBNST) by fast-scan cyclic voltammetry at carbon fiber microelectrodes. Pharmacological agents were introduced either by systemic application (intraperitoneal injection) or by local application (iontophoresis). The iontophoresis barrels were attached to a carbon fiber microelectrode to allow simultaneous detection of evoked NE release and quantitation of iontophoretic delivery. Desipramine (DMI), an inhibitor of NET, increased evoked release and slowed clearance of released NE in both regions independent of the mode of delivery. However, the effects of DMI were more robust in the vBNST than in the dmBNST. Similarly, the α2-AR autoreceptor inhibitor idazoxan (IDA) enhanced NE release in both regions but to a greater extent in the vBNST by both modes of delivery. Since both local application by iontophoresis and systemic application of IDA had similar effects on NE release, our results indicate that terminal autoreceptors play a predominant role in the inhibition of subsequent release.

scholarly journals Construction and Implementation of Carbon Fiber Microelectrode Arrays for Chronic and Acute In Vivo Recordings

10.3791/62760 ◽  
2021 ◽  
Author(s):  
Kristen N. Reikersdorfer ◽  
Andrea K. Stacy ◽  
David A. Bressler ◽  
Lauren S. Hayashi ◽  
Keith B. Hengen ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Microelectrode Arrays ◽  
Carbon Fiber Microelectrode

In Vivo Brain Glucose Measurements:  Differential Normal Pulse Voltammetry with Enzyme-Modified Carbon Fiber Microelectrodes

1996 ◽  
Vol 68 (24) ◽  
pp. 4358-4364 ◽  
Author(s):  
Larissa I. Netchiporouk ◽  
Nataliya F. Shram ◽  
Nicole Jaffrezic-Renault ◽  
Claude Martelet ◽  
Raymond Cespuglio
Keyword(s):  
Carbon Fiber ◽  
Brain Glucose ◽  
Pulse Voltammetry ◽  
Carbon Fiber Microelectrodes
Sign in / Sign up

Export Citation Format

Share Document