2-dimensional potassium ion image sensor aiming for Label-free dynamic imaging of living-cell activity

2009 ◽  
Author(s):  
K. Atsumi ◽  
Y. Masaki ◽  
T. Noda ◽  
T. Hattori ◽  
H. Takao ◽  
...  
Keyword(s):  
Living Cell ◽  
Cell Activity ◽  
Image Sensor ◽  
Potassium Ion ◽  
Dynamic Imaging ◽  
Label Free ◽  
Free Dynamic

Label free bio image sensor for real time monitoring of potassium ion released from hippocampal slices

2014 ◽  
Vol 201 ◽  
pp. 439-443 ◽  
Author(s):  
Akiteru Kono ◽  
Takashi Sakurai ◽  
Toshiaki Hattori ◽  
Koichi Okumura ◽  
Makoto Ishida ◽  
...  
Keyword(s):  
Real Time ◽  
Hippocampal Slices ◽  
Image Sensor ◽  
Potassium Ion ◽  
Label Free ◽  
Real Time Monitoring

scholarly journals DNA Aptamer Functionalized Hydrogels for Interferometric Fiber-Optic Based Continuous Monitoring of Potassium Ions

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 266
Author(s):  
Nataša Žuržul ◽  
Bjørn Torger Stokke
Keyword(s):  
Conformational Transition ◽  
Polymer Network ◽  
Potassium Ion ◽  
Ion Concentration ◽  
Dna Aptamer ◽  
Blood Levels ◽  
Label Free ◽  
Practical Applications ◽  
Hydrogel Matrix ◽  
K Concentration

In the present paper, we describe a potassium sensor based on DNA-aptamer functionalized hydrogel, that is capable of continuous label-free potassium ion (K+) monitoring with potential for in situ application. A hydrogel attached to the end of an optical fiber is designed with di-oligonucleotides grafted to the polymer network that may serve as network junctions in addition to the covalent crosslinks. Specific affinity toward K+ is based on exploiting a particular aptamer that exhibits conformational transition from single-stranded DNA to G-quadruplex formed by the di-oligonucleotide in the presence of K+. Integration of this aptamer into the hydrogel transforms the K+ specific conformational transition to a K+ concentration dependent deswelling of the hydrogel. High-resolution interferometry monitors changes in extent of swelling at 1 Hz and 2 nm resolution for the hydrogel matrix of 50 µm. The developed hydrogel-based biosensor displayed high selectivity for K+ ions in the concentration range up to 10 mM, in the presence of physiological concentrations of Na+. Additionally, the concentration dependent and selective K+ detection demonstrated in the artificial blood buffer environment, both at room and physiological temperatures, suggests substantial potential for practical applications such as monitoring of potassium ion concentration in blood levels in intensive care medicine.

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

2011 ◽  
Vol 6 (11) ◽  
pp. 1748-1760 ◽  
Author(s):  
Ralf Schröder ◽  
Johannes Schmidt ◽  
Stefanie Blättermann ◽  
Lucas Peters ◽  
Nicole Janssen ◽  
...  
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Label Free ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled ◽  
Free Dynamic

Applying label-free dynamic mass redistribution assay for studying endogenous FPR1 receptor signalling in human neutrophils

2017 ◽  
Vol 88 ◽  
pp. 72-78 ◽  
Author(s):  
Hanna B. Christensen ◽  
David E. Gloriam ◽  
Daniel Sejer Pedersen ◽  
Jack B. Cowland ◽  
Niels Borregaard ◽  
...  
Keyword(s):  
Human Neutrophils ◽  
Label Free ◽  
Dynamic Mass ◽  
Receptor Signalling ◽  
Mass Redistribution ◽  
Free Dynamic

scholarly journals Versatile live-cell activity analysis platform for characterization of neuronal dynamics at single-cell and network level

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Xinyue Yuan ◽  
Manuel Schröter ◽  
Marie Engelene J. Obien ◽  
Michele Fiscella ◽  
Wei Gong ◽  
...  
Keyword(s):  
Neuronal Networks ◽  
Microelectrode Array ◽  
Cell Activity ◽  
Live Cell ◽  
High Signal ◽  
Neuronal Cultures ◽  
Label Free ◽  
Fluorescent Indicators ◽  
Analysis Platform

AbstractChronic imaging of neuronal networks in vitro has provided fundamental insights into mechanisms underlying neuronal function. Current labeling and optical imaging methods, however, cannot be used for continuous and long-term recordings of the dynamics and evolution of neuronal networks, as fluorescent indicators can cause phototoxicity. Here, we introduce a versatile platform for label-free, comprehensive and detailed electrophysiological live-cell imaging of various neurogenic cells and tissues over extended time scales. We report on a dual-mode high-density microelectrode array, which can simultaneously record in (i) full-frame mode with 19,584 recording sites and (ii) high-signal-to-noise mode with 246 channels. We set out to demonstrate the capabilities of this platform with recordings from primary and iPSC-derived neuronal cultures and tissue preparations over several weeks, providing detailed morpho-electrical phenotypic parameters at subcellular, cellular and network level. Moreover, we develop reliable analysis tools, which drastically increase the throughput to infer axonal morphology and conduction speed.

B201 Label-free and real-time CARS imaging of living cell reactions in laser-induced ablation

2009 ◽  
Vol 2009.20 (0) ◽  
pp. 93-94
Author(s):  
Takeo MINAMIKAWA ◽  
Hirohiko NIIOKA ◽  
Tsutomu ARAKI ◽  
Mamoru HASHIMOTO
Keyword(s):  
Real Time ◽  
Living Cell ◽  
Label Free ◽  
Cars Imaging
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