Label-Free Dynamic Imaging of Chromatin in Live Cell Nuclei by High-Speed Scattering-Based Interference Microscopy

ACS Nano ◽  
2021 ◽  
Author(s):  
Yi-Teng Hsiao ◽  
Chia-Ni Tsai ◽  
Te-Hsin Chen ◽  
Chia-Lung Hsieh
Keyword(s):  
High Speed ◽  
Dynamic Imaging ◽  
Live Cell ◽  
Interference Microscopy ◽  
Label Free ◽  
Cell Nuclei ◽  
Free Dynamic

scholarly journals Microscopic scan-free surface profiling over extended axial ranges by point-spread-function engineering

2020 ◽  
Vol 6 (44) ◽  
pp. eabc0332
Author(s):  
Racheli Gordon-Soffer ◽  
Lucien E. Weiss ◽  
Ran Eshel ◽  
Boris Ferdman ◽  
Elias Nehme ◽  
...  
Keyword(s):  
Free Surface ◽  
Point Spread Function ◽  
High Speed ◽  
Label Free ◽  
Surface Profiling ◽  
Leverage Point ◽  
Point Spread ◽  
Spread Function ◽  
Point Spread Function Engineering ◽  
Free Dynamic

The shape of a surface, i.e., its topography, influences many functional properties of a material; hence, characterization is critical in a wide variety of applications. Two notable challenges are profiling temporally changing structures, which requires high-speed acquisition, and capturing geometries with large axial steps. Here, we leverage point-spread-function engineering for scan-free, dynamic, microsurface profiling. The presented method is robust to axial steps and acquires full fields of view at camera-limited framerates. We present two approaches for implementation: fluorescence-based and label-free surface profiling, demonstrating the applicability to a variety of sample geometries and surface types.

Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

2011 ◽  
Vol 6 (11) ◽  
pp. 1748-1760 ◽  
Author(s):  
Ralf Schröder ◽  
Johannes Schmidt ◽  
Stefanie Blättermann ◽  
Lucas Peters ◽  
Nicole Janssen ◽  
...  
Keyword(s):  
G Protein ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Label Free ◽  
Dynamic Mass ◽  
Mass Redistribution ◽  
G Protein Coupled ◽  
Free Dynamic

scholarly journals Monitoring Reactivation of Latent HIV by Label-Free Gradient Light Interference Microscopy

iScience ◽  
2021 ◽  
pp. 102940
Author(s):  
Neha Goswami ◽  
Yiyang Lu ◽  
Mikhail E. Kandel ◽  
Michael J. Fanous ◽  
Kathrin Bohn-Wippert ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Label Free ◽  
Light Interference ◽  
Latent Hiv

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Simultaneous AFM and FLIM Imaging with a SiR-DNA Probe Reveals Structural Changes during DNA Condensation in Live Cell Nuclei

2018 ◽  
Vol 114 (3) ◽  
pp. 596a
Author(s):  
Chetan Poudel ◽  
Nathan Curry ◽  
Kevin A. Feeney ◽  
Gabriele S. Kaminski Schierle ◽  
Clemens F. Kaminski
Keyword(s):  
Structural Changes ◽  
Dna Condensation ◽  
Dna Probe ◽  
Live Cell ◽  
Cell Nuclei

scholarly journals High-speed synthetic aperture microscopy for live cell imaging

2011 ◽  
Vol 36 (2) ◽  
pp. 148 ◽  
Author(s):  
Moonseok Kim ◽  
Youngwoon Choi ◽  
Christopher Fang-Yen ◽  
Yongjin Sung ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Synthetic Aperture ◽  
Synthetic Aperture Microscopy
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