A lab-on-a-chip for free-flow electrophoretic preconcentration of viruses and gel electrophoretic DNA extraction

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2554-2561
Author(s):  
Matthias Hügle ◽  
Ole Behrmann ◽  
Madlen Raum ◽  
Frank T. Hufert ◽  
Gerald A. Urban ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Lab On A Chip ◽  
Free Flow

A lab-on-a-chip for FFE preconcentration of viruses and gel electrophoretic DNA extraction: complete preparation of amplifiable DNA from dilute specimens.

scholarly journals A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 537 ◽  
Author(s):  
Cancan Zhu ◽  
Anzhong Hu ◽  
Junsheng Cui ◽  
Ke Yang ◽  
Xinchao Zhu ◽  
...  
Keyword(s):  
High Risk ◽  
Dna Extraction ◽  
Molecular Diagnostics ◽  
Solid Phase ◽  
Lab On A Chip ◽  
Clinical Diagnostics ◽  
Oligonucleotide Array ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Multiplex Amplification

Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.

scholarly journals A lab-on-a-chip for preconcentration of bacteria and nucleic acid extraction

RSC Advances ◽  
2018 ◽  
Vol 8 (36) ◽  
pp. 20124-20130 ◽  
Author(s):  
M. Hügle ◽  
G. Dame ◽  
O. Behrmann ◽  
R. Rietzel ◽  
D. Karthe ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Gel Electrophoresis ◽  
Lab On A Chip ◽  
Free Flow ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

A lab-on-a-chip combining free-flow electrophoretic preconcentration and thermoelectric lysis of bacteria as well as purification of nucleic acids by gel-electrophoresis.

Pre-storage of liquid reagents in glass ampoules for DNA extraction on a fully integrated lab-on-a-chip cartridge

Lab on a Chip ◽  
2010 ◽  
Vol 10 (11) ◽  
pp. 1480 ◽  
Author(s):  
Jochen Hoffmann ◽  
Daniel Mark ◽  
Sascha Lutz ◽  
Roland Zengerle ◽  
Felix von Stetten
Keyword(s):  
Dna Extraction ◽  
Lab On A Chip ◽  
Fully Integrated

The free flow of ideas, information and materials

2000 ◽  
Vol 24 (6) ◽  
pp. iii-iii
Author(s):  
Natasha V. Raikhel ◽  
Mikio Nishimura ◽  
Ralph S. Quatrano ◽  
Stephen H. Howell ◽  
William Davies ◽  
...  
Keyword(s):  
Free Flow

Purification of plasma membranes from leaves of conifer and deciduous tree species by phase partitioning and free-flow electrophoresis

1995 ◽  
Vol 95 (3) ◽  
pp. 399-408 ◽  
Author(s):  
Elena Toll ◽  
Federico J. Castillo ◽  
Pierre Crespi ◽  
Michele Crevecoeur ◽  
Hubert Greppin
Keyword(s):  
Tree Species ◽  
Plasma Membranes ◽  
Free Flow ◽  
Deciduous Tree ◽  
Phase Partitioning

Eine Plasmaextraktion durch Free-Flow-Elektrophorese ermöglicht die selektive Bestimmung von Insulinanaloga in Gegenwart von endogenem Normalinsulin

2008 ◽  
Vol 3 (S 1) ◽  
Author(s):  
A Pfützner ◽  
M Safinowski ◽  
M Nissum ◽  
U Sukop ◽  
G Weber ◽  
...  
Keyword(s):  
Free Flow

Relationship of HLA-DRB1 Alleles and Rheumatoid Arthritis in West South of Iran

2017 ◽  
Vol 9 (03) ◽  
Author(s):  
Karim Mowla ◽  
Elham Rajaee M. D. ◽  
Mehrdad Dargahi-MalAmir M. D. ◽  
Neda Yousefinezhad ◽  
Maryam Jamali Hondori
Keyword(s):  
Rheumatoid Arthritis ◽  
Environmental Factors ◽  
Rheumatoid Factor ◽  
Dna Extraction ◽  
Wrist Joint ◽  
Blood Sampling ◽  
Healthy Controls ◽  
Relationship Of ◽  
Synovial Pannus

Background: Rheumatoid arthritis is a systemic multifactor disease that presented with symmetrical polyarthritis more preferably in small wrist joint and ankle. Synovial pannus cause destruction and deformities in joints. The main reason of this disease in unknown, but past researchesshowed that genetically factor play important role beside environmental factors in susceptibility to this entity. Method:100 patients with rheumatoid arthritis diagnosed upon ACR 2010 criteria enrolled study. 92 healthy patents also enrolled DNA studying. of both group was extracted through DNA extraction kits by blood sampling. HLA-DRB1 typing was done by PCR-SSP method. Results: There were no significant differences in HLADRB1 *04, HLADRB1*08 and HLADRB1*11 alleles presentation between patients and healthy controls. Only there were statically significant correlation between HLA-DRB1*08 and Rheumatoid factor positive patents. (P = 0.025).

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