Self-focusing chips for size-dependent DNA separation in nonuniformly distributed asymmetric electric fields

Author(s):  
Soyeon Yi ◽  
Kyoung-Sun Seo ◽  
Young-Ho Cho
2006 ◽  
Author(s):  
Armin Kiessling ◽  
Anne Rausch ◽  
Vladislav Matusevich ◽  
Richard Kowarschik

2019 ◽  
Author(s):  
Terje Wimberger ◽  
Verena K. Köhler ◽  
Eva K. Ehmoser ◽  
Klemens J. Wassermann

AbstractIrreversible electroporation holds great potential for cell-specific lysis due to the size-dependent susceptibility of cells to externally imposed electric fields. Previous attempts at selective cell lysis lead to significant overlap between affected populations and struggle with inconsistent biological outcome. We propose that charge transfer at the electrode-liquid interface is responsible by inducing multifactorial effects originating from both the electric field and electrochemical reactions. A promising remedy is the coating of electrodes with a high-k dielectric layer. The resulting capacitive coupling restores the selective potential of electric field mediated lysis in a microfluidic setup. Initial experiments show the consistent depletion of erythrocytes from whole blood while leaving leukocytes intact. The same is true for the reproducible and selective depletion of Jurkat and MCF-7 cells in a mixture with leukocytes. Unexpectedly, the observed order of lysis cannot be correlated with cell size. This implies that the cellular response to capacitive coupling features a selective characteristic that is different from conventional lysis configurations.


2019 ◽  
Vol 99 ◽  
pp. 02011
Author(s):  
Joseph R. Toth ◽  
Siddharth Rajupet ◽  
Henry Squire ◽  
Blaire Volbers ◽  
Jùn Zhou ◽  
...  

It is well known that electric fields occur in wind-blown dust, due to the triboelectric charging of particles as they collide. Triboelectric charging, or contact electrification, is a poorly understood and complex phenomenon. It is especially important in granular systems, as the high surface-to-volume ratio can lead to the build-up of large amounts of charge. A particularly surprising effect, which is important in dust systems, is that charge transfer occurs in systems of a single composition, such that there is a particle-size dependent polarity of the particles. Here, we use a combined experimental and theoretical approach to elucidate the electrostatic charging that occurs during dust storms, and the effects of this electrostatic charging on dust transport. We create laboratory-scale wind-blown dust systems, and study the electrostatic charging. We find that larger particles tend to charge positive and to stay at or near the sand bed, while smaller particles tend to charge negative and get lofted to higher elevations. This self-segregating of charged particles would lead to electric fields within a dust storm. Our results show that electric fields then increase the dust transport by more easily lofting charged particles.


Biopolymers ◽  
1991 ◽  
Vol 31 (2) ◽  
pp. 253-254 ◽  
Author(s):  
Toshiyuki Shikata ◽  
Tadao Kotaka

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