A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics

Lab on a Chip ◽  
2008 ◽  
Vol 8 (12) ◽  
pp. 2121 ◽  
Author(s):  
Soo Hyun Lee ◽  
Sung-Woo Kim ◽  
Ji Yoon Kang ◽  
Chong H. Ahn
Keyword(s):  
Reverse Transcription ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Clinical Diagnostics ◽  
Rt Pcr

scholarly journals Elasto-Magnetic Pumps Integrated within Microfluidic Devices

2021 ◽  
Vol 4 (1) ◽  
pp. 48
Author(s):  
Jacob L. Binsley ◽  
Elizabeth L. Martin ◽  
Thomas O. Myers ◽  
Stefano Pagliara ◽  
Feodor Y. Ogrin
Keyword(s):  
Magnetic System ◽  
Point Of Care ◽  
Microfluidic Channel ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Point Of Care Testing ◽  
Ongoing Research ◽  
Flow Rates ◽  
Pumping System ◽  
Oscillating Magnetic Field

Many lab-on-a-chip devices require a connection to an external pumping system in order to perform their function. While this is not problematic in typical laboratory environments, it is not always practical when applied to point-of-care testing, which is best utilized outside of the laboratory. Therefore, there has been a large amount of ongoing research into producing integrated microfluidic components capable of generating effective fluid flow from on-board the device. This research aims to introduce a system that can produce practical flow rates, and be easily fabricated and actuated using readily available techniques and materials. We show how an asymmetric elasto-magnetic system, inspired by Purcell’s three-link swimmer, can provide this solution through the generation of non-reciprocal motion in an enclosed environment. The device is fabricated monolithically within a microfluidic channel at the time of manufacture, and is actuated using a weak, oscillating magnetic field. The flow rate can be altered dynamically, and the direction of the resultant flow can be controlled by adjusting the frequency of the driving field. The device has been proven, experimentally and numerically, to operate effectively when applied to fluids with a range of viscosities. Such a device may be able to replace external pumping systems in portable applications.

scholarly journals Developments in Transduction, Connectivity and AI/Machine Learning for Point-of-Care Testing

Sensors ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1917 ◽  
Author(s):  
Shane O’Sullivan ◽  
Zulfiqur Ali ◽  
Xiaoyi Jiang ◽  
Reza Abdolvand ◽  
M Selim Ünlü ◽  
...  
Keyword(s):  
Machine Learning ◽  
Data Analytics ◽  
Point Of Care ◽  
Developed Countries ◽  
Clinical Diagnostics ◽  
Least Developed Countries ◽  
Point Of Care Testing ◽  
Middle Income ◽  
Lab On Chip ◽  
On Chip

We review some emerging trends in transduction, connectivity and data analytics for Point-of-Care Testing (POCT) of infectious and non-communicable diseases. The patient need for POCT is described along with developments in portable diagnostics, specifically in respect of Lab-on-chip and microfluidic systems. We describe some novel electrochemical and photonic systems and the use of mobile phones in terms of hardware components and device connectivity for POCT. Developments in data analytics that are applicable for POCT are described with an overview of data structures and recent AI/Machine learning trends. The most important methodologies of machine learning, including deep learning methods, are summarised. The potential value of trends within POCT systems for clinical diagnostics within Lower Middle Income Countries (LMICs) and the Least Developed Countries (LDCs) are highlighted.

scholarly journals Proceedings of the First Workshop on Standards for Microfluidics

2019 ◽  
Vol 124 ◽  
Author(s):  
Darwin R. Reyes ◽  
Henne van Heeren
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
In Vitro Diagnostics ◽  
Point Of Care Testing ◽  
Future Developments ◽  
Exciting Opportunity ◽  
Micro Total Analysis Systems ◽  
Advance Research ◽  
Total Analysis

In the last two decades, the microfluidics/lab-on-a-chip field has evolved from the concept of micro total analysis systems, where systems with integrated pretreatment and analysis of chemicals were envisioned, to what is known today as lab-on-a-chip, which is expected to be modular. This field has shown great potential for the development of technologies that can make, and to some extent are making, a big difference in areas such as in vitro diagnostics, point of care testing, organ on a chip, and many more. Microfluidics plays an essential role in these systems, and determining the standards needed in this area is critical for enabling new markets and products, and to advance research and development. Our goal was to bring together stakeholders from industry, academia, and government to discuss and define the needs within the field for the development of standards. This publication contains a summary of the workshop, abstracts from each presentation, and a summary of the breakout sessions from the National Institute of Standards and Technology Workshop on Standards for Microfluidics, held on June 1–2, 2017. The workshop was attended by 46 persons from 26 organizations and 11 countries. This was a unique and exciting opportunity for stakeholders from all over the world to join in the discussion of future developments towards standardization in the microfluidics arena.

Point-of-care testing (POCT) diagnostic systems using microfluidic lab-on-a-chip technologies

2015 ◽  
Vol 132 ◽  
pp. 46-57 ◽  
Author(s):  
Wooseok Jung ◽  
Jungyoup Han ◽  
Jin-Woo Choi ◽  
Chong H. Ahn
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Diagnostic Systems ◽  
Point Of Care Testing

Development of functional lab-on-a-chip on polymer for point-of-care testing of metabolic parameters

Lab on a Chip ◽  
2008 ◽  
Vol 8 (12) ◽  
pp. 2113 ◽  
Author(s):  
Jaephil Do ◽  
Sehwan Lee ◽  
Jungyup Han ◽  
Junhai Kai ◽  
Chien-Chong Hong ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Metabolic Parameters ◽  
Point Of Care Testing

Disposable Smart Lab on a Chip for Point-of-Care Clinical Diagnostics

2004 ◽  
Vol 92 (1) ◽  
pp. 154-173 ◽  
Author(s):  
C.H. Ahn ◽  
J.-W. Choi ◽  
G. Beaucage ◽  
J. Nevin ◽  
J.-B. Lee ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Clinical Diagnostics

scholarly journals An ultraportable and versatile point-of-care DNA testing platform

2020 ◽  
Vol 6 (17) ◽  
pp. eaaz7445 ◽  
Author(s):  
Huan Xu ◽  
Anyue Xia ◽  
Dandan Wang ◽  
Yiheng Zhang ◽  
Shaoli Deng ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Point Of Care ◽  
Clinical Diagnostics ◽  
Dna Testing ◽  
Point Of Care Testing ◽  
Resource Limited Settings ◽  
Single Base ◽  
Signal Detector ◽  
Resource Limited ◽  
Different Types

Point-of-care testing (POCT) has broad applications in resource-limited settings. Here, a POCT platform termed POCKET (point-of-care kit for the entire test) is demonstrated that is ultraportable and versatile for analyzing multiple types of DNA in different fields in a sample-to-answer manner. The POCKET is less than 100 g and smaller than 25 cm in length. The kit consists of an integrated chip (i-chip) and a foldable box (f-box). The i-chip integrates the sample preparation with a previously unidentified, triple signal amplification. The f-box uses a smartphone as a heater, a signal detector, and a result readout. We detected different types of DNA from clinics to environment to food to agriculture. The detection is sensitive (<103 copies/ml), specific (single-base differentiation), speedy (<2 hours), and stable (>10 weeks shelf life). This inexpensive, ultraportable POCKET platform may become a versatile sample-to-answer platform for clinical diagnostics, food safety, agricultural protection, and environmental monitoring.

scholarly journals Polymer microfluidic devices: an overview of fabrication methods

2017 ◽  
Vol 1 (1) ◽  
pp. 67-79 ◽  
Author(s):  
Raquel O. Rodrigues ◽  
Rui Lima ◽  
Helder T. Gomes ◽  
Adrián M. T. Silva
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Lab On A Chip ◽  
Microfluidic Devices ◽  
Point Of Care Testing ◽  
Standard Methods ◽  
Time Consumption ◽  
Advantages And Disadvantages ◽  
Fundamental Research ◽  
Microfabrication Techniques

The amount of applications associated with microfluidic devices is increasing since the introduction of Lab-on-a-chip devices in the 1990s, especially regarding biomedical and clinical fields. However, in order for this technology to leave the fundamental research and become a day-life technology (e.g., as point-of-care testing), it needs to be disposable and reasonably less expensive. Polymers, due to their several advantages, such as easier microfabrication and low-cost, fill these needs. Several methods are reported regarding microfabrication and, thus, the main aim of the present work is to provide an overview of the most relevant microfabrication techniques found in literature employing polymers, clarifying also the main advantages and disadvantages of each technique and especially considering their cost and time-consumption. Moreover, a future outlook of low-cost microfabrication techniques and standard methods is provided.

Sign in / Sign up

Export Citation Format

Share Document