Disposable Smart Lab on a Chip for Point-of-Care Clinical Diagnostics

2004 ◽  
Vol 92 (1) ◽  
pp. 154-173 ◽  
Author(s):  
C.H. Ahn ◽  
J.-W. Choi ◽  
G. Beaucage ◽  
J. Nevin ◽  
J.-B. Lee ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Clinical Diagnostics

A polymer lab-on-a-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics

Lab on a Chip ◽  
2008 ◽  
Vol 8 (12) ◽  
pp. 2121 ◽  
Author(s):  
Soo Hyun Lee ◽  
Sung-Woo Kim ◽  
Ji Yoon Kang ◽  
Chong H. Ahn
Keyword(s):  
Reverse Transcription ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Clinical Diagnostics ◽  
Rt Pcr

scholarly journals Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3985
Author(s):  
Nan Wan ◽  
Yu Jiang ◽  
Jiamei Huang ◽  
Rania Oueslati ◽  
Shigetoshi Eda ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Capacitive Sensing ◽  
Application Potential ◽  
Mirna Detection ◽  
Mirna 25 ◽  
Low Sensitivity

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

scholarly journals Clinical Applications of Visual Plasmonic Colorimetric Sensing

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6214
Author(s):  
Elba Mauriz
Keyword(s):  
Point Of Care ◽  
Clinical Diagnostics ◽  
Localized Surface Plasmon ◽  
Visible Range ◽  
Future Perspectives ◽  
Colorimetric Analysis ◽  
Colorimetric Sensing ◽  
Size Dependent ◽  
Clinical Biomarkers ◽  
Naked Eye Detection

Colorimetric analysis has become of great importance in recent years to improve the operationalization of plasmonic-based biosensors. The unique properties of nanomaterials have enabled the development of a variety of plasmonics applications on the basis of the colorimetric sensing provided by metal nanoparticles. In particular, the extinction of localized surface plasmon resonance (LSPR) in the visible range has permitted the exploitation of LSPR colorimetric-based biosensors as powerful tools for clinical diagnostics and drug monitoring. This review summarizes recent progress in the biochemical monitoring of clinical biomarkers by ultrasensitive plasmonic colorimetric strategies according to the distance- or the morphology/size-dependent sensing modes. The potential of colorimetric nanosensors as point of care devices from the perspective of naked-eye detection is comprehensively discussed for a broad range of analytes including pharmaceuticals, proteins, carbohydrates, nucleic acids, bacteria, and viruses such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The practical suitability of plasmonic-based colorimetric assays for the rapid visual readout in biological samples, considering current challenges and future perspectives, is also reviewed.

‘Off–on’ switchable fluorescent probe for prompt and cost-efficient detection of bacteria

2019 ◽  
Vol 43 (33) ◽  
pp. 13094-13102
Author(s):  
Giorgia Giovannini ◽  
Vladimir Gubala ◽  
Andrew J. Hall
Keyword(s):  
Fluorescent Probe ◽  
Bacterial Infections ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Human Samples ◽  
Efficient Detection ◽  
Prevention And Treatment ◽  
Cost Efficient

The rapid and straightforward detection of bacteria in food and human samples is becoming important, particularly in view of the development of point-of-care devices and lab-on-a-chip tools for prevention and treatment of bacterial infections.

scholarly journals Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 220 ◽  
Author(s):  
Niazul I. Khan ◽  
Edward Song
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Review Article ◽  
Microfluidic Chips ◽  
High Affinity ◽  
Sensing Platforms ◽  
Personal Medicine ◽  
Paper Based Microfluidics ◽  
Recognition Elements ◽  
Analyte Detection

Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

Lab-on-a-Chip Device and System for Point-of-Care Applications

2017 ◽  
pp. 87-121
Author(s):  
Tsung-Feng Wu ◽  
Sung Hwan Cho ◽  
Yu-Jui Chiu ◽  
Yu-Hwa Lo
Keyword(s):  
Point Of Care ◽  
Lab On A Chip

Project-Based Learning: Microfluidic Lab-on-a-Chip Point-of-Care Medical Tests for Engineering Undergraduates

2017 ◽  
Author(s):  
Michael G. Mauk ◽  
Richard Y. Chiou ◽  
Carlos Ruiz ◽  
Dharma Varapula ◽  
Changchun Liu ◽  
...  
Keyword(s):  
Undergraduate Students ◽  
Systems Engineering ◽  
Engineering Students ◽  
Undergraduate Research ◽  
Point Of Care ◽  
Lab On A Chip ◽  
Medical Diagnostics ◽  
Green Technology ◽  
Project Based Learning ◽  
Microfluidic Chips

Point-of-care (POC) medical diagnostics tests based on instrumented microfluidic chips are instructive and highly-multidisciplinary projects for undergraduate research and Senior Design. Students can apply their knowledge of fluid mechanics, heat transfer, optics, electronics and microcontrollers, materials, prototyping and systems engineering in translating and adapting a laboratory-based test for use in non-traditional venues. We discuss the design, prototyping, and testing of POC lab-on-a-chip (LOC) systems in an educational setting, where undergraduate students develop and demonstrate novel and practical POC tests. This application area serves as an effective gateway to the medical diagnostics field for engineering students, with opportunities for providing sustainable, appropriate, and ‘green’ technology to the developing world where healthcare infrastructure is lacking.

scholarly journals A Lab-on-a-Chip Device Integrated DNA Extraction and Solid Phase PCR Array for the Genotyping of High-Risk HPV in Clinical Samples

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 537 ◽  
Author(s):  
Cancan Zhu ◽  
Anzhong Hu ◽  
Junsheng Cui ◽  
Ke Yang ◽  
Xinchao Zhu ◽  
...  
Keyword(s):  
High Risk ◽  
Dna Extraction ◽  
Molecular Diagnostics ◽  
Solid Phase ◽  
Lab On A Chip ◽  
Clinical Diagnostics ◽  
Oligonucleotide Array ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Multiplex Amplification

Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The ingenious design of the pneumatic microvalves enabled the fluid mixing and reagent storage to be organically combined, significantly reducing the size of the chip. The solid oligonucleotide array incorporated into the chip allowed the spatial separation of the primers and minimized undesirable interactions in multiplex amplification. As a proof-of-concept for POC molecular diagnostics on the device, five genotypes of high-risk human papillomavirus (HPV) (HPV16/HPV18/HPV31/HPV33/HPV58) were examined. Positive quality control samples and HPV patient cervical swab specimens were analyzed on the integrated microdevice. The platform was capable of detection approximately 50 copies of HPV virus per reaction during a single step, including DNA extraction, solid-phase PCR and genotype detection, in 1 h from samples being added to the chip. This simple and inexpensive microdevice provided great utility for the screening and monitoring of HPV genotypes. The sample-to-result platform will pave the way for wider application of POC molecular testing in the fields of clinical diagnostics, food safety, and environmental monitoring.

Development of a magnetic lab-on-a-chip for point-of-care sepsis diagnosis

2009 ◽  
Vol 321 (10) ◽  
pp. 1671-1675 ◽  
Author(s):  
Joerg Schotter ◽  
Astrit Shoshi ◽  
Hubert Brueckl
Keyword(s):  
Point Of Care ◽  
Lab On A Chip ◽  
Sepsis Diagnosis
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