High-throughput fabrication of sub-micron pillar arrays for free-solution DNA electrophoresis without E-beam lithography

2005 ◽  
Author(s):  
Yick Chuen Chan ◽  
Yi-Kuen Lee ◽  
Man Wong ◽  
Y. Zohar
Keyword(s):  
High Throughput ◽  
Free Solution ◽  
Dna Electrophoresis ◽  
Pillar Arrays

Effects of embedded sub-micron pillar arrays in microfluidic channels on large DNA electrophoresis

2009 ◽  
Vol 30 (18) ◽  
pp. 3242-3249 ◽  
Author(s):  
Yick Chuen Chan ◽  
Yitshak Zohar ◽  
Yi-Kuen Lee
Keyword(s):  
Microfluidic Channels ◽  
Dna Electrophoresis ◽  
Pillar Arrays

scholarly journals Size-dependent free solution DNA electrophoresis in structured microfluidic systems

2003 ◽  
Vol 67-68 ◽  
pp. 905-912 ◽  
Author(s):  
Thanh Tu Duong ◽  
Gyuman Kim ◽  
Robert Ros ◽  
Martin Streek ◽  
Friederike Schmid ◽  
...  
Keyword(s):  
Free Solution ◽  
Microfluidic Systems ◽  
Dna Electrophoresis ◽  
Size Dependent

Microscopic behaviour of DNA during electrophoresis: electrophoretic orientation

1991 ◽  
Vol 24 (2) ◽  
pp. 103-164 ◽  
Author(s):  
Bengt Nordén ◽  
Christer Elvingson ◽  
Mats Jonsson ◽  
Björn Åkerman
Keyword(s):  
Molecular Biology ◽  
Electric Field ◽  
Electric Fields ◽  
Gel Electrophoresis ◽  
Kerr Effect ◽  
Free Solution ◽  
Dna Electrophoresis ◽  
Research Fields ◽  
Physico Chemical ◽  
Dynamical Properties

The study of the behaviour of DNA when subjected to electric fields poses several intriguing problems of fundamental physico-chemical importance. Electric field (Kerr effect) orientation of DNA in free solution as well as migration of DNA in gel electrophoresis are two well-established, but so far rather separate, research fields. Whereas the first one has been generally concerned with basic structural and dynamical properties of DNA (Charney, 1988), the second is closely related to techniques of molecular biology (for a review on DNA electrophoresis, see stellwagen 1987).

Accurate, High-Throughput "Snapshot" Detection of hMLHl Mutations by Two-Dimensional DNA Electrophoresis

1998 ◽  
Vol 2 (1) ◽  
pp. 43-53 ◽  
Author(s):  
WENDY M. SMITH ◽  
NATHALIE J. VAN ORSOUW ◽  
EDWARD A. FOX ◽  
RICHARD D. KOLODNER ◽  
JAN VIJG ◽  
...  
Keyword(s):  
High Throughput ◽  
Two Dimensional ◽  
Dna Electrophoresis

scholarly journals High-throughput cryo-EM enabled by user-free preprocessing routines

2019 ◽  
Author(s):  
Yilai Li ◽  
Jennifer N. Cash ◽  
John. J.G. Tesmer ◽  
Michael A. Cianfrocco
Keyword(s):  
Image Analysis ◽  
Deep Learning ◽  
Structural Biology ◽  
High Throughput ◽  
Data Preprocessing ◽  
Free Solution ◽  
Multiple Datasets ◽  
User Friendly ◽  
Collection Strategies ◽  
Automatic Pipeline

ABSTRACTThe growth of single-particle cryo-EM into a mainstream structural biology tool has allowed for many important biological discoveries. Continued developments in data collection strategies alongside new sample preparation devices heralds a future where users will collect multiple datasets per microscope session. To make cryo-EM data processing more automatic and user-friendly, we have developed an automatic pipeline for cryo-EM data preprocessing and assessment using a combination of deep learning and image analysis tools. We have verified the performance of this pipeline on a number of datasets and extended its scope to include sample screening by the user-free assessment of the qualities of a series of datasets under different conditions. We propose that our workflow provides a decision-free solution for cryo-EM, making data preprocessing more generalized and robust in the high-throughput era as well as more convenient for users from a range of backgrounds.

scholarly journals Simultaneously Sizing and Quantitating Zeptomole-Level DNA at High Throughput in Free Solution

2014 ◽  
Vol 20 (43) ◽  
pp. 13821-13821
Author(s):  
Zaifang Zhu ◽  
Huang Chen ◽  
Apeng Chen ◽  
Joann J. Lu ◽  
Shaorong Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Free Solution
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