Effects of embedded sub-micron pillar arrays in microfluidic channels on large DNA electrophoresis

2009 ◽  
Vol 30 (18) ◽  
pp. 3242-3249 ◽  
Author(s):  
Yick Chuen Chan ◽  
Yitshak Zohar ◽  
Yi-Kuen Lee
Keyword(s):  
Microfluidic Channels ◽  
Dna Electrophoresis ◽  
Pillar Arrays

scholarly journals Microfluidic Preconcentration Chip with Self-Assembled Chemical Modified Surface for Trace Carbonyl Compounds Detection

Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4402 ◽  
Author(s):  
Jie Cheng ◽  
Jianwei Shao ◽  
Yifei Ye ◽  
Yang Zhao ◽  
Chengjun Huang ◽  
...  
Keyword(s):  
Water Quality ◽  
Self Assembly ◽  
Carbonyl Compounds ◽  
Microelectromechanical Systems ◽  
Fem Analysis ◽  
Microfluidic Channels ◽  
Chemical Surface ◽  
Chemical Surface Modification ◽  
Pillar Arrays ◽  
Low Sensitivity

Carbonyl compounds in water sources are typical characteristic pollutants, which are important indicators in the health risk assessment of water quality. Commonly used analytical chemistry methods face issues such as complex operations, low sensitivity, and long analysis times. Here, we report a silicon microfluidic device based on click chemical surface modification that was engineered to achieve rapid, convenient and efficient capture of trace level carbonyl compounds in liquid solvent. The micro pillar arrays of the chip and microfluidic channels were designed under the basis of finite element (FEM) analysis and fabricated by the microelectromechanical systems (MEMS) technique. The surface of the micropillars was sputtered with precious metal silver and functionalized with the organic substance amino-oxy dodecane thiol (ADT) by self-assembly for capturing trace carbonyl compounds. The detection of ppb level fluorescent carbonyl compounds demonstrates that the strategy proposed in this work shows great potential for rapid water quality testing and for other samples with trace carbonyl compounds.

scholarly journals Automated Parallel Pattern Search Optimisation of Microfluidic Geometry for Extracellular Vesicle Liquid Biopsies

2021 ◽  
Author(s):  
Colin L Hisey ◽  
AJ Tyler ◽  
Arvin Lim ◽  
Lawrence W Chamley ◽  
Cherie Blenkiron ◽  
...  
Keyword(s):  
High Performance ◽  
Disease Diagnosis ◽  
Extracellular Vesicle ◽  
Pattern Search ◽  
Microfluidic Channels ◽  
Liquid Biopsies ◽  
Pillar Arrays ◽  
Unit Cells ◽  
Parallel Pattern ◽  
Elution Method

Microfluidic liquid biopsies using affinity-based capture of extracellular vesicles (EVs) have demonstrated great potential for providing rapid disease diagnosis and monitoring. However, little effort has been devoted to optimising the geometry of the microfluidic channels for maximum EV capture due to the inherent challenges of physically testing many geometric designs. To address this, we developed an automated parallel pattern search (PPS) optimiser by combining a Python optimiser, COMSOL Multiphysics, and high performance computing. This unique approach was applied to a triangular micropillar array geometry by parameterising repeating unit cells, making several assumptions, and optimising for maximum particle capture efficiency. We successfully optimised the triangular pillar arrays and surprisingly found that simply maximising the total number of pillars and effective surface area did not result in maximum EV capture, as devices with slightly larger pillars and more spacing between pillars allowed contact with slower moving EVs that followed the pillar contours more closely. We then experimentally validated this finding using bioreactor-produced EVs in the best and worst channel designs that were functionalised with an antibody against CD63. Captured EVs were quantified using a fluorescent plate reader, followed by an established elution method and nanoparticle tracking analysis. These results demonstrate the power of automated microfluidic geometry optimisations for EV liquid biopsies and will support further development of this rapidly growing field.

JOULE HEATING INDUCED HEAT TRANSFER IN ELECTROKINETIC FLOW THROUGH MICROFLUIDIC CHANNELS

Equipment ◽  
2006 ◽  
Author(s):  
C. Yang ◽  
G. Y. Tang ◽  
D. G. Yan ◽  
H. Q. Gong ◽  
John C. Chai ◽  
...  
Keyword(s):  
Heat Transfer ◽  
Joule Heating ◽  
Microfluidic Channels ◽  
Electrokinetic Flow ◽  
Flow Through

Stochastic Computing via In Operando Modulation of Rectification in Molecular Tunneling Junctions

2020 ◽  
Author(s):  
Xinkai Qiu ◽  
Sylvia Rousseva ◽  
Gang Ye ◽  
Jan C. Hummelen ◽  
Ryan Chiechi
Keyword(s):  
Self Assembly ◽  
Variable Temperature ◽  
Self Assembled Monolayers ◽  
Microfluidic Channels ◽  
Proof Of Concept ◽  
Glycol Ethers ◽  
Stochastic Computing ◽  
Assembled Monolayers ◽  
In Operando ◽  
Tunneling Junctions

This paper describes the reconfiguration of molecular tunneling junctions during operation via the self-assembly of bilayers of glycol ethers. We use well-established functional groups to modulate the magnitude and direction of rectification in assembled tunneling junctions by exposing them to solutions containing different glycol ethers. Variable-temperature measurements establish that rectification occurs by a bias-dependent tunneling-hopping mechanism and that glycol ethers, beside being an unusually efficient tunneling medium, behave identically to alkanes. We fabricated memory bits from crossbar junctions prepared by injecting eutectic Ga-In into microfluidic channels. Two 8-bit registers were able to perform logical AND operations on bit strings encoded into chemical packets as microfluidic droplets that alter the composition of the crossbar junctions through self-assembly to effect memristor-like properties. This proof of concept work demonstrates the potential for fieldable molecular-electronic devices based on tunneling junctions of self-assembled monolayers and bilayers.

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