scholarly journals Clinical Applications of Visual Plasmonic Colorimetric Sensing

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6214
Author(s):  
Elba Mauriz
Keyword(s):  
Point Of Care ◽  
Clinical Diagnostics ◽  
Localized Surface Plasmon ◽  
Visible Range ◽  
Future Perspectives ◽  
Colorimetric Analysis ◽  
Colorimetric Sensing ◽  
Size Dependent ◽  
Clinical Biomarkers ◽  
Naked Eye Detection

Colorimetric analysis has become of great importance in recent years to improve the operationalization of plasmonic-based biosensors. The unique properties of nanomaterials have enabled the development of a variety of plasmonics applications on the basis of the colorimetric sensing provided by metal nanoparticles. In particular, the extinction of localized surface plasmon resonance (LSPR) in the visible range has permitted the exploitation of LSPR colorimetric-based biosensors as powerful tools for clinical diagnostics and drug monitoring. This review summarizes recent progress in the biochemical monitoring of clinical biomarkers by ultrasensitive plasmonic colorimetric strategies according to the distance- or the morphology/size-dependent sensing modes. The potential of colorimetric nanosensors as point of care devices from the perspective of naked-eye detection is comprehensively discussed for a broad range of analytes including pharmaceuticals, proteins, carbohydrates, nucleic acids, bacteria, and viruses such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The practical suitability of plasmonic-based colorimetric assays for the rapid visual readout in biological samples, considering current challenges and future perspectives, is also reviewed.

scholarly journals Colorimetric Sensing of Pb2+ Ion by Using Ag Nanoparticles in the Presence of Dithizone

Chemosensors ◽  
2019 ◽  
Vol 7 (3) ◽  
pp. 28 ◽  
Author(s):  
Roto Roto ◽  
Bella Mellisani ◽  
Agus Kuncaka ◽  
Mudasir Mudasir ◽  
Adhitasari Suratman
Keyword(s):  
Ag Nanoparticles ◽  
Localized Surface Plasmon Resonance ◽  
Heavy Metal Ions ◽  
Limit Of Detection ◽  
Good Alternative ◽  
Localized Surface Plasmon ◽  
Colorimetric Analysis ◽  
Colorimetric Sensing ◽  
Limit Of Quantification ◽  
A Minor

Colorimetric analysis of heavy metal ions can be realized by the aid of Ag nanoparticles to improve the analytical characteristics. The method is based on the localized surface plasmon resonance (LSPR) properties of the Ag nanoparticles (AgNPs). In this work, we applied the AgNPs with the addition of dithizone to further improve the selectivity and sensitivity of Pb2+ analysis. Colorimetric sensing of Pb2+ ions based on the polyvinyl alcohol (PVA)-stabilized-colloidal AgNPs in the presence of dithizone is reported. A linear decrease in the AgNPs LSPR absorbance at 421 nm was observed along with the increase in the Pb2+ concentration in the range of 0.50–10 µg/L. The other ions give a minor change in the LSPR absorbance of colloidal AgNPs. The Pb2+ limit of detection, the limit of quantification, and sensitivity were found to be 0.64 ± 0.04 µg/L, 2.1 ± 0.15 µg/L, 0.0282 ± 0.0040 L/µg (n = 5), respectively. The obtained sensitivity is comparable with that of the immunosensing method. The proposed method could offer a good alternative for colorimetric analysis of Pb2+ ions by using nanoparticles in the presence of ligands, which can improve selectivity.

scholarly journals Rapid and Sensitive Detection of miRNA Based on AC Electrokinetic Capacitive Sensing for Point-of-Care Applications

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3985
Author(s):  
Nan Wan ◽  
Yu Jiang ◽  
Jiamei Huang ◽  
Rania Oueslati ◽  
Shigetoshi Eda ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Point Of Care ◽  
Low Cost ◽  
Clinical Diagnostics ◽  
Detection Methods ◽  
Capacitive Sensing ◽  
Application Potential ◽  
Mirna Detection ◽  
Mirna 25 ◽  
Low Sensitivity

A sensitive and efficient method for microRNAs (miRNAs) detection is strongly desired by clinicians and, in recent years, the search for such a method has drawn much attention. There has been significant interest in using miRNA as biomarkers for multiple diseases and conditions in clinical diagnostics. Presently, most miRNA detection methods suffer from drawbacks, e.g., low sensitivity, long assay time, expensive equipment, trained personnel, or unsuitability for point-of-care. New methodologies are needed to overcome these limitations to allow rapid, sensitive, low-cost, easy-to-use, and portable methods for miRNA detection at the point of care. In this work, to overcome these shortcomings, we integrated capacitive sensing and alternating current electrokinetic effects to detect specific miRNA-16b molecules, as a model, with the limit of detection reaching 1.0 femto molar (fM) levels. The specificity of the sensor was verified by testing miRNA-25, which has the same length as miRNA-16b. The sensor we developed demonstrated significant improvements in sensitivity, response time and cost over other miRNA detection methods, and has application potential at point-of-care.

scholarly journals A 3D-printed magnetic digital microfluidic diagnostic platform for rapid colorimetric sensing of carbapenemase-producing Enterobacteriaceae

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Pojchanun Kanitthamniyom ◽  
Pei Yun Hon ◽  
Aiwu Zhou ◽  
Mohammad Yazid Abdad ◽  
Zhi Yun Leow ◽  
...  
Keyword(s):  
Point Of Care ◽  
Resistance Mechanism ◽  
Turnaround Time ◽  
World Health ◽  
Colorimetric Sensing ◽  
Resource Limited ◽  
Reaction Chambers ◽  
Technological Platform ◽  
Health Organization ◽  
Digital Microfluidic

AbstractCarbapenemase-producing Enterobacteriaceae (CPE) are a group of drug-resistant Gram-negative pathogens that are classified as a critical threat by the World Health Organization (WHO). Conventional methods of detecting antibiotic-resistant pathogens do not assess the resistance mechanism and are often time-consuming and laborious. We have developed a magnetic digital microfluidic (MDM) platform, known as MDM Carba, for the identification of CPE by measuring their ability to hydrolyze carbapenem antibiotics. MDM Carba offers the ability to rapidly test CPE and reduce the amount of reagents used compared with conventional phenotypic testing. On the MDM Carba platform, tests are performed in droplets that function as reaction chambers, and fluidic operations are accomplished by manipulating these droplets with magnetic force. The simple droplet-based magnetic fluidic operation allows easy system automation and simplified hands-on operation. Because of the unique “power-free” operation of MDM technology, the MDM Carba platform can also be operated manually, showing great potential for point-of-care testing in resource-limited settings. We tested 27 bacterial isolates on the MDM Carba platform, and the results showed sensitivity and specificity that were comparable to those of the widely used Carba NP test. MDM Carba may shorten the overall turnaround time for CPE identification, thereby enabling more timely clinical decisions for better clinical outcomes. MDM Carba is a technological platform that can be further developed to improve diagnostics for other types of antibiotic resistance with minor modifications.

scholarly journals Current role of ultrasound in hemodialysis access evaluation

2021 ◽  
pp. 112972982110346
Author(s):  
Meola Mario ◽  
Jose Ibeas ◽  
Jan Malik
Keyword(s):  
Turning Point ◽  
Point Of Care ◽  
Low Cost ◽  
Wireless Systems ◽  
Future Perspectives ◽  
Maturation Period ◽  
Surveillance Programs ◽  
Dialysis Nurses

Physical examination (PE) is considered the backbone before vascular access (VA) placement, during maturation period and for follow-up. However, it may be inadequate in identifying suitable vasculature, mainly in comorbid patients, or in detecting complications. This review highlights the advantages of ultrasound imaging to manage VA before placement, during maturation and follow-up. Furthermore, it analyses the future perspectives in evaluating early and late VA complications thank to the availability of multiparametric platforms, point of care of ultrasound, and portable/wireless systems. Technical improvements and low-cost systems should favor the widespread ultrasound-based VA surveillance programs. This significant turning point needs an adequate training of nephrologists and dialysis nurses and the standardization of exams, parameters, and procedures.

scholarly journals The trends of uveal melanoma research in the past two decades and future perspectives.

2021 ◽  
Author(s):  
Khaled Ali Elubous ◽  
Ali Daoud Alebous ◽  
Hebah Ali Abous ◽  
Rawan Ali Elubous
Keyword(s):  
Uveal Melanoma ◽  
Web Of Science ◽  
The United States ◽  
Future Research ◽  
Genetic Mutations ◽  
Future Perspectives ◽  
Molecular Alterations ◽  
The Past ◽  
Clinical Biomarkers

Abstract PURPOSE Evaluation of the research trends in uveal melanoma in the past two decades.METHODS Data were extracted from the Web of Science database website. VOSviewer and Citespace software were used to analyze the retrieved data. RESULTS The leading country in terms of output and international collaboration is the United States. Research interest in genetic mutations, molecular pathways, and immunotherapy was remarkable in recent years. Most of the top ten journals are specialized in ophthalmology. In recent years the hotspots include future perspectives, BAB1 mutation, therapeutic target, and systematic reviews. The keywords with the strongest citation bursts are immunotherapy, outcome, and in situ hybridization. CONCLUSION The output of uveal melanoma research increased during the past two decades. Future research foci may include exploring different mutations role, immunotherapy, molecular alterations, and finding ideal clinical biomarkers.

scholarly journals Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review

Sensors ◽  
2019 ◽  
Vol 19 (24) ◽  
pp. 5435 ◽  
Author(s):  
Zhanhong Li ◽  
Mona A. Mohamed ◽  
A. M. Vinu Mohan ◽  
Zhigang Zhu ◽  
Vinay Sharma ◽  
...  
Keyword(s):  
Environmental Monitoring ◽  
Biomedical Application ◽  
Analytical Techniques ◽  
Clinical Diagnostics ◽  
Cardiac Biomarkers ◽  
Research Progress ◽  
Electrochemical Biosensors ◽  
Recognition Element ◽  
Clinical Biomarkers ◽  
Electrochemical Biosensing

Aptamers are synthetic bio-receptors of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) origin selected by the systematic evolution of ligands (SELEX) process that bind a broad range of target analytes with high affinity and specificity. So far, electrochemical biosensors have come up as a simple and sensitive method to utilize aptamers as a bio-recognition element. Numerous aptamer based sensors have been developed for clinical diagnostics, food, and environmental monitoring and several other applications are under development. Aptasensors are capable of extending the limits of current analytical techniques in clinical diagnostics, food, and environmental sample analysis. However, the potential applications of aptamer based electrochemical biosensors are unlimited; current applications are observed in the areas of food toxins, clinical biomarkers, and pesticide detection. This review attempts to enumerate the most representative examples of research progress in aptamer based electrochemical biosensing principles that have been developed in recent years. Additionally, this account will discuss various current developments on aptamer-based sensors toward heavy metal detection, for various cardiac biomarkers, antibiotics detection, and also on how the aptamers can be deployed to couple with antibody-based assays as a hybrid sensing platform. Aptamers can be used in various applications, however, this account will focus on the recent advancements made toward food, environmental, and clinical diagnostic application. This review paper compares various electrochemical aptamer based sensor detection strategies that have been applied so far and used as a state of the art. As illustrated in the literature, aptamers have been utilized extensively for environmental, cancer biomarker, biomedical application, and antibiotic detection and thus have been extensively discussed in this article.

scholarly journals Colorimetric Detection of Sulfide Anions via Redox-Modulated Surface Chemistry and Morphology of Au-Hg Nanorods

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Xiaotian Zhu ◽  
Chang Liu ◽  
Jie Liu
Keyword(s):  
Surface Chemistry ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Localized Surface Plasmon ◽  
Wavelength Shift ◽  
Colorimetric Sensing ◽  
Leather Industry ◽  
Colorimetric Response ◽  
Sensitivity And Selectivity

A new colorimetric assay for the detection of sulfide anions with high sensitivity and selectivity is reported, utilizing Au-Hg alloy nanorods (Au-HgNRs) as probe. Au-HgNRs were prepared by modifying gold nanorods (AuNRs) with reducing agent and mercury ions. In an aqueous solution with sulfide anions, the formation of mercuric sulfide due to redox reaction between the amalgams and sulfide anions greatly changed the surface chemistry and morphology of the Au-HgNRs, leading to a red shift of the localized surface plasmon resonance (LSPR) absorption peak, accompanied by a change in colorimetric response. A good linear relationship was obtained between the LSPR peak wavelength shift and concentration of sulfide anion in the range of 1 × 10−5−1 × 10−4 mol/L. The selectivity of this method has been investigated by other anions. The colorimetric sensing system successfully detected sulfide in wastewater from leather industry.

scholarly journals Acoustofluidic Micromixing Enabled Hybrid Integrated Colorimetric Sensing, for Rapid Point-of-Care Measurement of Salivary Potassium

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 73 ◽  
Author(s):  
Vikram Surendran ◽  
Thomas Chiulli ◽  
Swetha Manoharan ◽  
Stephen Knisley ◽  
Muthukumaran Packirisamy ◽  
...  
Keyword(s):  
Color Change ◽  
Point Of Care ◽  
Sample Volume ◽  
Point Of Care Testing ◽  
Colorimetric Sensing ◽  
In Situ Tests ◽  
Detection Scheme ◽  
Chip Technology ◽  
Whole Saliva ◽  
Microfluidic Biosensor

The integration of microfluidics with advanced biosensor technologies offers tremendous advantages such as smaller sample volume requirement and precise handling of samples and reagents, for developing affordable point-of-care testing methodologies that could be used in hospitals for monitoring patients. However, the success and popularity of point-of-care diagnosis lies with the generation of instantaneous and reliable results through in situ tests conducted in a painless, non-invasive manner. This work presents the development of a simple, hybrid integrated optical microfluidic biosensor for rapid detection of analytes in test samples. The proposed biosensor works on the principle of colorimetric optical absorption, wherein samples mixed with suitable chromogenic substrates induce a color change dependent upon the analyte concentration that could then be detected by the absorbance of light in its path length. This optical detection scheme has been hybrid integrated with an acoustofluidic micromixing unit to enable uniform mixing of fluids within the device. As a proof-of-concept, we have demonstrated the real-time application of our biosensor format for the detection of potassium in whole saliva samples. The results show that our lab-on-a-chip technology could provide a useful strategy in biomedical diagnoses for rapid analyte detection towards clinical point-of-care testing applications.

scholarly journals Colorimetric Nanoplasmonics to Spot Hyperglycemia From Saliva

2020 ◽  
Vol 8 ◽  
Author(s):  
Paolo Donati ◽  
Tania Pomili ◽  
Luca Boselli ◽  
Pier P. Pompa
Keyword(s):  
Large Scale ◽  
Point Of Care ◽  
High Sensitivity ◽  
Diabetic Patients ◽  
Visual Response ◽  
Colorimetric Sensing ◽  
Chronic Patients ◽  
Self Monitoring ◽  
Sensing Platform ◽  
Signal Color

Early diagnostics and point-of-care (POC) devices can save people’s lives or drastically improve their quality. In particular, millions of diabetic patients worldwide benefit from POC devices for frequent self-monitoring of blood glucose. Yet, this still involves invasive sampling processes, which are quite discomforting for frequent measurements, or implantable devices dedicated to selected chronic patients, thus precluding large-scale monitoring of the globally increasing diabetic disorders. Here, we report a non-invasive colorimetric sensing platform to identify hyperglycemia from saliva. We designed plasmonic multibranched gold nanostructures, able to rapidly change their shape and color (naked-eye detection) in the presence of hyperglycemic conditions. This “reshaping approach” provides a fast visual response and high sensitivity, overcoming common detection issues related to signal (color intensity) losses and bio-matrix interferences. Notably, optimal performances of the assay were achieved in real biological samples, where the biomolecular environment was found to play a key role. Finally, we developed a dipstick prototype as a rapid home-testing kit.

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