A novel tip-surface distance control for low temperature scanning near-field photoluminescence spectroscopy

2005 ◽  
Author(s):  
A. Crottini ◽  
J.L. Staehli ◽  
B. Deveaud ◽  
X.L. Wang ◽  
M. Ogura
Keyword(s):  
Low Temperature ◽  
Near Field ◽  
Photoluminescence Spectroscopy ◽  
Surface Distance ◽  
Distance Control ◽  
Temperature Scanning

A low-temperature scanning near-field optical microscope for photoluminescence at semiconductor structures

1998 ◽  
Vol 66 (7) ◽  
pp. S381-S384 ◽  
Author(s):  
I. Manke ◽  
D. Pahlke ◽  
J. Lorbacher ◽  
W. Busse ◽  
T. Kalka ◽  
...  
Keyword(s):  
Low Temperature ◽  
Near Field ◽  
Optical Microscope ◽  
Semiconductor Structures ◽  
Temperature Scanning

Low temperature scanning-tip microwave near-field microscopy of YBa2Cu3O7−x films

1997 ◽  
Vol 71 (14) ◽  
pp. 2026-2028 ◽  
Author(s):  
I. Takeuchi ◽  
T. Wei ◽  
Fred Duewer ◽  
Y. K. Yoo ◽  
X.-D. Xiang ◽  
...  
Keyword(s):  
Low Temperature ◽  
Near Field ◽  
Temperature Scanning

A low-temperature scanning confocal and near-field optical microscope

1997 ◽  
Vol 68 (6) ◽  
pp. 2466-2474 ◽  
Author(s):  
W. Göhde ◽  
J. Tittel ◽  
Th. Basché ◽  
C. Bräuchle ◽  
U. C. Fischer ◽  
...  
Keyword(s):  
Low Temperature ◽  
Near Field ◽  
Optical Microscope ◽  
Temperature Scanning

Near-field low-temperature photoluminescence spectroscopy of single V-shaped quantum wires

1999 ◽  
Vol 60 (19) ◽  
pp. 13335-13338 ◽  
Author(s):  
V. Emiliani ◽  
Ch. Lienau ◽  
M. Hauert ◽  
G. Colí ◽  
M. DeGiorgi ◽  
...  
Keyword(s):  
Low Temperature ◽  
Quantum Wires ◽  
Near Field ◽  
Photoluminescence Spectroscopy ◽  
Low Temperature Photoluminescence

Low Temperature Near-Field Photoluminescence Spectroscopy of InGaAs Single Quantum Dots

1998 ◽  
Vol 37 (Part 1, No. 3B) ◽  
pp. 1638-1642 ◽  
Author(s):  
Toshiharu Saiki ◽  
Kenichi Nishi ◽  
Motoichi Ohtsu
Keyword(s):  
Quantum Dots ◽  
Low Temperature ◽  
Near Field ◽  
Photoluminescence Spectroscopy ◽  
Single Quantum ◽  
Single Quantum Dots

KRIOSBOM101 low-temperature scanning near-field optical microscope

2010 ◽  
Vol 37 (9) ◽  
pp. 276-279 ◽  
Author(s):  
M. G. Petrova ◽  
G. V. Mishakov ◽  
E. I. Demikhov ◽  
A. I. Sharkov
Keyword(s):  
Low Temperature ◽  
Near Field ◽  
Optical Microscope ◽  
Temperature Scanning

Intracellular structures of rapid frozen biological samples observed by high-resolution low-temperature Scanning Electron Microscopy

1989 ◽  
Vol 47 ◽  
pp. 736-737
Author(s):  
T. Inoué ◽  
H. Koike
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Low Temperature ◽  
Liquid Nitrogen ◽  
Specimen Preparation ◽  
Chemical Fixation ◽  
Brown Adipose ◽  
Critical Point Drying ◽  
Temperature Scanning ◽  
Scanning Electron

Low temperature scanning electron microscopy (LTSEM) is useful to avoid artifacts such as deformation and extraction, because specimens are not subjected to chemical fixation, dehydration and critical-point drying. Since Echlin et al developed a LTSEM, many techniques and instruments have been reported for observing frozen materials. However, intracellular structures such as mitochondria and endoplasmic reticulum have been unobservable by the method because of the low resolving power and inadequate specimen preparation methods. Recently, we developed a low temperature SEM that attained high resolutions. In this study, we introduce highly magnified images obtained by the newly developed LTSEM, especially intracellular structures which have been rapidly frozen without chemical fixation.[Specimen preparations] Mouse pancreas and brown adipose tissues (BAT) were used as materials. After the tissues were removed and cut into small pieces, the specimen was placed on a cryo-tip and rapidly frozen in liquid propane using a rapid freezing apparatus (Eiko Engineering Co. Ltd., Japan). After the tips were mounted on the specimen stage of a precooled cryo-holder, the surface of the specimen was manually fractured by a razor blade in liquid nitrogen. The cryo-holder was then inserted into the specimen chamber of the SEM (ISI DS-130), and specimens were observed at the accelerating voltages of 5-8 kV. At first the surface was slightly covered with frost, but intracellular structures were gradually revealed as the frost began to sublimate. Gold was then coated on the specimen surface while tilting the holder at 45-90°. The holder was connected to a liquid nitrogen reservoir by means of a copper braid to maintain low temperature.

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