Optical trapping assisted enhancement of on-chip single molecule detection rate with a solid-state nanopore

2017 ◽  
Author(s):  
M. Rahman ◽  
M. Harrington ◽  
M. A. Stott ◽  
T. D. Yuzvinsky ◽  
Y. Li ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Detection Rate ◽  
Optical Trapping ◽  
Single Molecule Detection ◽  
On Chip

Measuring Trapped DNA at the Liquid-Air Interface for Enhanced Single Molecule Sensing

Nanoscale ◽  
2021 ◽  
Author(s):  
Nasim Farajpour ◽  
Lauren Lastra ◽  
Vinay Sharma ◽  
Kevin Freedman
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Borosilicate Glass ◽  
Low Noise ◽  
Viable Alternative ◽  
Single Molecule Detection ◽  
Widespread Application ◽  
Promising Tool ◽  
Air Interface ◽  
Cost Efficient

Nanopore sensing is a promising tool with widespread application in single-molecule detection. Borosilicate glass nanopores are a viable alternative to other solid-state nanopores due to low noise and cost-efficient fabrication....

SEM-induced shrinking of solid-state nanopores for single molecule detection

2011 ◽  
Vol 22 (42) ◽  
pp. 425302 ◽  
Author(s):  
Anmiv S Prabhu ◽  
Kevin J Freedman ◽  
Joseph W F Robertson ◽  
Zhorro Nikolov ◽  
John J Kasianowicz ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Single Molecule Detection

scholarly journals On-Chip Single Molecule Detection of Unlabeled DNA Targets

2010 ◽  
Vol 98 (3) ◽  
pp. 183a
Author(s):  
Anders Gunnarsson ◽  
Peter Sjövall ◽  
Peter Jönsson ◽  
Fredrik Höök
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
On Chip

Shrinking Graphene Nanopore Using Electron-Beam-Induced Deposition for Single Molecule Detection

2015 ◽  
Author(s):  
Jian Ma ◽  
Weiwei Zhao ◽  
Lei Liu ◽  
Jingjie Sha ◽  
Yunfei Chen
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Solid State ◽  
Single Molecule ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Single Molecule Detection ◽  
Large Size ◽  
Scanning Electron ◽  
Graphene Nanopore

Solid-state nanopore has already shown success of single molecule detection and graphene nanopore is potential for successful DNA sequencing. Here, we present a fast and controllable way to fabricate sub-5 nm nanopore on graphene membrane. The process includes two steps: sputtering a large size nanopore using a conventional focused ion beam (FIB) and shrinking the large nanopore to a few nanometers using scanning electron microscope (SEM). We also demonstrated the ability of the graphene nanopores fabricated in this manner to detect individual 48Kbp λ-DNA molecules.

scholarly journals Precise Control of Solid-State Nanopore Device Properties for Single-Molecule Detection Applications

2013 ◽  
Vol 104 (2) ◽  
pp. 520a
Author(s):  
Furat Sawafta ◽  
Bason Clancy ◽  
Martin Huber ◽  
Adam R. Hall
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Precise Control ◽  
Device Properties

Recent advances in integrated solid-state nanopore sensors

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Md. Mahmudur Rahman ◽  
Mohammad Julker Neyen Sampad ◽  
Aaron Hawkins ◽  
Holger Schmidt
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Life Science ◽  
New Class ◽  
Recent Advances ◽  
On Chip ◽  
Nanopore Sensors

The advent of single-molecule probing techniques has revolutionized the biomedical and life science fields and has spurred the development of a new class of labs-on-chip based on powerful biosensors. Nanopores...

scholarly journals Screening for Group A Streptococcal disease via Solid-State Nanopore Detection of PCR Amplicons

2021 ◽  
Author(s):  
Simon King ◽  
Kyle Briggs ◽  
Robert Slinger ◽  
Vincent Tabard-Cossa
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Group A Streptococcus ◽  
Single Molecule Detection ◽  
Detection Methods ◽  
Clinical Samples ◽  
Great Promise ◽  
Early Detection Of Disease ◽  
Group A ◽  
Analytical Approaches

Single molecule detection methods are becoming increasingly important for diagnostic applications. Practical Early detection of disease requires sensitivity down to the level of single copies of the targeted biomarkers. Of the candidate technologies that can address this need, solid-state nanopores show great promise as digital sensors for single-molecule detection. Here, we present work detailing the use of solid-state nanopores as downstream sensors for a PCR-based assay targeting group A streptococcus (strep A) which can be readily extended to detect any pathogen that can be identified with a short nucleic acid sequence. We demonstrate that with some simple modifications to the standard PCR reaction mixture, nanopores can be used to reliably identify strep A in clinical samples. We also discuss methodological best practices both for adapting PCR-based assays to solid-state nanopore readout as well as analytical approaches by which to decide on sample status.

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