Measuring Trapped DNA at the Liquid-Air Interface for Enhanced Single Molecule Sensing

Nanoscale ◽  
2021 ◽  
Author(s):  
Nasim Farajpour ◽  
Lauren Lastra ◽  
Vinay Sharma ◽  
Kevin Freedman
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Borosilicate Glass ◽  
Low Noise ◽  
Viable Alternative ◽  
Single Molecule Detection ◽  
Widespread Application ◽  
Promising Tool ◽  
Air Interface ◽  
Cost Efficient

Nanopore sensing is a promising tool with widespread application in single-molecule detection. Borosilicate glass nanopores are a viable alternative to other solid-state nanopores due to low noise and cost-efficient fabrication....

SEM-induced shrinking of solid-state nanopores for single molecule detection

2011 ◽  
Vol 22 (42) ◽  
pp. 425302 ◽  
Author(s):  
Anmiv S Prabhu ◽  
Kevin J Freedman ◽  
Joseph W F Robertson ◽  
Zhorro Nikolov ◽  
John J Kasianowicz ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Single Molecule Detection

scholarly journals Comparing current noise in biological and solid-state nanopores

2019 ◽  
Author(s):  
A. Fragasso ◽  
S. Schmid ◽  
C. Dekker
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Ionic Current ◽  
Low Noise ◽  
Signal To Noise ◽  
Experimental Conditions ◽  
High Frequencies ◽  
Noise Ratio

AbstractNanopores bear great potential as single-molecule tools for bioanalytical sensing and sequencing, due to their exceptional sensing capabilities, high-throughput, and low cost. The detection principle relies on detecting small differences in the ionic current as biomolecules traverse the nanopore. A major bottleneck for the further progress of this technology is the noise that is present in the ionic current recordings, because it limits the signal-to-noise ratio and thereby the effective time resolution of the experiment. Here, we review the main types of noise at low and high frequencies and discuss the underlying physics. Moreover, we compare biological and solid-state nanopores in terms of the signal-to-noise ratio (SNR), the important figure of merit, by measuring free translocations of a short ssDNA through a selected set of nanopores under typical experimental conditions. We find that SiNx solid-state nanopores provide the highest SNR, due to the large currents at which they can be operated and the relatively low noise at high frequencies. However, the real game-changer for many applications is a controlled slowdown of the translocation speed, which for MspA was shown to increase the SNR >160-fold. Finally, we discuss practical approaches for lowering the noise for optimal experimental performance and further development of the nanopore technology.

Shrinking Graphene Nanopore Using Electron-Beam-Induced Deposition for Single Molecule Detection

2015 ◽  
Author(s):  
Jian Ma ◽  
Weiwei Zhao ◽  
Lei Liu ◽  
Jingjie Sha ◽  
Yunfei Chen
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Solid State ◽  
Single Molecule ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Single Molecule Detection ◽  
Large Size ◽  
Scanning Electron ◽  
Graphene Nanopore

Solid-state nanopore has already shown success of single molecule detection and graphene nanopore is potential for successful DNA sequencing. Here, we present a fast and controllable way to fabricate sub-5 nm nanopore on graphene membrane. The process includes two steps: sputtering a large size nanopore using a conventional focused ion beam (FIB) and shrinking the large nanopore to a few nanometers using scanning electron microscope (SEM). We also demonstrated the ability of the graphene nanopores fabricated in this manner to detect individual 48Kbp λ-DNA molecules.

scholarly journals Precise Control of Solid-State Nanopore Device Properties for Single-Molecule Detection Applications

2013 ◽  
Vol 104 (2) ◽  
pp. 520a
Author(s):  
Furat Sawafta ◽  
Bason Clancy ◽  
Martin Huber ◽  
Adam R. Hall
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Precise Control ◽  
Device Properties

scholarly journals Screening for Group A Streptococcal disease via Solid-State Nanopore Detection of PCR Amplicons

2021 ◽  
Author(s):  
Simon King ◽  
Kyle Briggs ◽  
Robert Slinger ◽  
Vincent Tabard-Cossa
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Group A Streptococcus ◽  
Single Molecule Detection ◽  
Detection Methods ◽  
Clinical Samples ◽  
Great Promise ◽  
Early Detection Of Disease ◽  
Group A ◽  
Analytical Approaches

Single molecule detection methods are becoming increasingly important for diagnostic applications. Practical Early detection of disease requires sensitivity down to the level of single copies of the targeted biomarkers. Of the candidate technologies that can address this need, solid-state nanopores show great promise as digital sensors for single-molecule detection. Here, we present work detailing the use of solid-state nanopores as downstream sensors for a PCR-based assay targeting group A streptococcus (strep A) which can be readily extended to detect any pathogen that can be identified with a short nucleic acid sequence. We demonstrate that with some simple modifications to the standard PCR reaction mixture, nanopores can be used to reliably identify strep A in clinical samples. We also discuss methodological best practices both for adapting PCR-based assays to solid-state nanopore readout as well as analytical approaches by which to decide on sample status.

Chapter 4. Solid-state Nanopore Confinement for Single Molecule Detection

2020 ◽  
pp. 118-161
Author(s):  
Yao Lin ◽  
Qiao Li ◽  
Shao-Chuang Liu ◽  
Si-Min Lu ◽  
Yi-Lun Ying ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Nanopore Confinement

Fast and low-noise tunnelling current measurements for single-molecule detection in an electrolyte solution using insulator-protected nanoelectrodes

Nanoscale ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 4076-4081 ◽  
Author(s):  
Takanori Morikawa ◽  
Kazumichi Yokota ◽  
Makusu Tsutsui ◽  
Masateru Taniguchi
Keyword(s):  
Single Molecule ◽  
Electrolyte Solution ◽  
Low Noise ◽  
Single Molecule Detection ◽  
Tunnelling Current

scholarly journals Fabrication and Applications of Solid-State Nanopores

Sensors ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1886 ◽  
Author(s):  
Qi Chen ◽  
Zewen Liu
Keyword(s):  
Electron Beam ◽  
Solid State ◽  
Single Molecule ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Biological Molecules ◽  
Single Molecule Detection ◽  
Massively Parallel ◽  
Selective Transport ◽  
Synthetic Materials

Nanopores fabricated from synthetic materials (solid-state nanopores), platforms for characterizing biological molecules, have been widely studied among researchers. Compared with biological nanopores, solid-state nanopores are mechanically robust and durable with a tunable pore size and geometry. Solid-state nanopores with sizes as small as 1.3 nm have been fabricated in various films using engraving techniques, such as focused ion beam (FIB) and focused electron beam (FEB) drilling methods. With the demand of massively parallel sensing, many scalable fabrication strategies have been proposed. In this review, typical fabrication technologies for solid-state nanopores reported to date are summarized, with the advantages and limitations of each technology discussed in detail. Advanced shrinking strategies to prepare nanopores with desired shapes and sizes down to sub-1 nm are concluded. Finally, applications of solid-state nanopores in DNA sequencing, single molecule detection, ion-selective transport, and nanopatterning are outlined.

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