A New Stilbene Synthase Gene from Muscadine (Vitis rotundifolia) Grape Berry

2007 ◽  
Author(s):  
Hemanth KN. Vasanthaiah ◽  
Ramesh Katam ◽  
Sheikh M. Basha
Keyword(s):  
Stilbene Synthase ◽  
Grape Berry ◽  
Vitis Rotundifolia ◽  
Stilbene Synthase Gene

The molecular regulation of stilbene phytoalexin biosynthesis in Vitis vinifera during grape berry development

2000 ◽  
Vol 27 (7) ◽  
pp. 723 ◽  
Author(s):  
Anthony J. Bais ◽  
Peter J. Murphy ◽  
Ian B. Dry
Keyword(s):  
Gene Expression ◽  
Vitis Vinifera ◽  
Stilbene Synthase ◽  
Grape Berry ◽  
Molecular Regulation ◽  
Vitis Vinifera L ◽  
Uv Treatment ◽  
Grape Berries ◽  
Uv Induction ◽  
Phytoalexin Biosynthesis

The molecular regulation of stilbene phytoalexin biosynthesis in developing Vitis vinifera L. grape berries was investigated using a UV induction system. Berries were collected at 1, 5, 10 and 16 weeks post-flowering from the cultivars Shiraz, Semillon, Cabernet Sauvignon and Chardonnay and the skins analysed for resveratrol production following irradiation with UV-C light. The rate and maximal level of resveratrol accumulation increased markedly in berries sampled from 1–5 weeks post-flowering and then dramatically declined in maturing berries sampled from 10–16 weeks post-flowering in all cultivars. In berries sampled at 1 and 5 weeks post-flowering, maximal levels of resveratrol accumulation were recorded at incubation periods of 24 and 48 h respectively whereas maximal resveratrol levels were not recorded in week 16 berry skins until 72 h after UV-treatment. Gene expression analysis indicated that stilbene synthase (STS) mRNA accumulated within 4–8 h of UV treatment in berries sampled at 1 and 5 weeks post-flowering, but did not increase in week 16 berries until 24–48 h following UV-irradiation. Furthermore, the overall level of STS gene expression declined in berries sampled 10–16 weeks post-flowering. The results demonstrate that inducible stilbene accumulation in ripening grape berries is highly regulated at the level of STS gene transcription. This decline in inducible STS gene expression may be a major factor contributing to the increased susceptibility of ripening grape berries to Botrytis cinerea infection.

Constitutive Expression of a Grapevine Stilbene Synthase Gene in Transgenic Hop (Humulus lupulusL.) Yields Resveratrol and Its Derivatives in Substantial Quantities

2007 ◽  
Vol 55 (17) ◽  
pp. 7002-7009 ◽  
Author(s):  
Axel Schwekendiek ◽  
Otmar Spring ◽  
Arne Heyerick ◽  
Benjamin Pickel ◽  
Nicola T. Pitsch ◽  
...  
Keyword(s):  
Constitutive Expression ◽  
Stilbene Synthase ◽  
Stilbene Synthase Gene

scholarly journals Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression

2006 ◽  
Vol 19 (10) ◽  
pp. 1103-1112 ◽  
Author(s):  
Laurent Laquitaine ◽  
Eric Gomès ◽  
Julie François ◽  
Chloé Marchive ◽  
Stéphanie Pascal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Stilbene Synthase ◽  
Gus Activity ◽  
Cell Suspensions ◽  
Type I ◽  
Lipid Transfer ◽  
Tobacco Plants ◽  
Stilbene Synthase Gene

Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed.

Genetic transformation of a fruit-specific, highly expressed stilbene synthase gene from Chinese wild Vitis quinquangularis

Planta ◽  
2016 ◽  
Vol 243 (4) ◽  
pp. 1041-1053 ◽  
Author(s):  
Siyan Cheng ◽  
Xiaoqing Xie ◽  
Yan Xu ◽  
Chaohong Zhang ◽  
Xiping Wang ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Stilbene Synthase ◽  
Chinese Wild Vitis ◽  
Stilbene Synthase Gene
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