Constitutive Expression of a Grapevine Stilbene Synthase Gene in Transgenic Hop (Humulus lupulusL.) Yields Resveratrol and Its Derivatives in Substantial Quantities

2007 ◽  
Vol 55 (17) ◽  
pp. 7002-7009 ◽  
Author(s):  
Axel Schwekendiek ◽  
Otmar Spring ◽  
Arne Heyerick ◽  
Benjamin Pickel ◽  
Nicola T. Pitsch ◽  
...  
Keyword(s):  
Constitutive Expression ◽  
Stilbene Synthase ◽  
Stilbene Synthase Gene

scholarly journals Transcriptional regulation of stilbene synthases in grapevine germplasm differentially susceptible to downy mildew

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Mario Ciaffi ◽  
Anna Rita Paolacci ◽  
Marco Paolocci ◽  
Enrica Alicandri ◽  
Valentina Bigini ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Downy Mildew ◽  
Transcriptional Activation ◽  
Constitutive Expression ◽  
Stilbene Synthase ◽  
Disease Symptoms ◽  
Adaptive Resistance ◽  
Genes Encoding ◽  
The Impact ◽  
Stilbene Biosynthesis

Abstract Background To limit the impact of the downy mildew disease of grapevine and reduce the need to recur to chemical treatments, an effective strategy might be recovering adaptive resistance traits in both cultivated and wild V. vinifera germplasm. Considering that stilbenes represent the most important class of phytoalexins in the Vitaceae, the constitutive expression and transcriptional activation of all the functional members of the stilbene synthase gene family were analysed in a group of nine grapevine genotypes following artificial infection with the oomycete Plasmopara viticola, the causal agent of the disease. In addition, in the same genotypes we analyzed the expression of genes encoding for two transcription factors involved in the transcriptional regulation of the stilbene synthase genes, namely VvMYB14 and VvMYB15, and of genes encoding for chalcone synthases. Results Downy mildew incidence and severity ranged from nihil to high in the grapevine genotypes considered, being low to moderate in a subgroup of V. vinifera genotypes. The constitutive expression of the stilbene synthase genes as well as the extent of their transcriptional activation following P. viticola inoculation appeared to be inversely related to the proneness to develop disease symptoms upon infection. In a specular manner, following P. viticola inoculation all the chalcone synthase genes were up-regulated in the susceptible grapevine genotypes and down-regulated in the resistant ones. The infection brought by P. viticola appeared to elicit a co-ordinated and sequential transcriptional activation of distinct stilbene synthase genes subsets, each of which may be regulated by a distinct and specific MYB transcription factor. Conclusions The present results suggest that the induction of stilbene biosynthesis may contribute to the basal immunity against the downy mildew of grapevine, thus representing an adaptive resistance trait to recover, in both cultivated and wild V. vinifera germplasm. During the early stages of P. viticola infection, an antagonistic interaction between flavonol and stilbene biosynthesis might occur, whose outcome might determine the subsequent extent of disease symptoms. Further studies are needed to decipher the possible regulatory mechanisms involved in the antagonistic crosstalk between these two metabolic pathways in resistant and susceptible genotypes in response to P. viticola.

scholarly journals Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression

2006 ◽  
Vol 19 (10) ◽  
pp. 1103-1112 ◽  
Author(s):  
Laurent Laquitaine ◽  
Eric Gomès ◽  
Julie François ◽  
Chloé Marchive ◽  
Stéphanie Pascal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Stilbene Synthase ◽  
Gus Activity ◽  
Cell Suspensions ◽  
Type I ◽  
Lipid Transfer ◽  
Tobacco Plants ◽  
Stilbene Synthase Gene

Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed.

Genetic transformation of a fruit-specific, highly expressed stilbene synthase gene from Chinese wild Vitis quinquangularis

Planta ◽  
2016 ◽  
Vol 243 (4) ◽  
pp. 1041-1053 ◽  
Author(s):  
Siyan Cheng ◽  
Xiaoqing Xie ◽  
Yan Xu ◽  
Chaohong Zhang ◽  
Xiping Wang ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Stilbene Synthase ◽  
Chinese Wild Vitis ◽  
Stilbene Synthase Gene

Differential Expression Patterns Within the Grapevine Stilbene Synthase Gene Family Revealed Through Their Regulatory Regions

2018 ◽  
Vol 36 (2) ◽  
pp. 225-238 ◽  
Author(s):  
Constanza Chialva ◽  
Claudio Muñoz ◽  
María Miccono ◽  
Estefanía Eichler ◽  
Luciano Calderón ◽  
...  
Keyword(s):  
Gene Family ◽  
Differential Expression ◽  
Expression Patterns ◽  
Stilbene Synthase ◽  
Regulatory Regions ◽  
Stilbene Synthase Gene
Sign in / Sign up

Export Citation Format

Share Document