Zero-mode waveguides for single molecule analysis and fast DNA sequencing

2003 ◽  
Author(s):  
M. Levene ◽  
J. Korlach ◽  
S. Turner ◽  
H.G. Craighead ◽  
W.W. Webb
Keyword(s):  
Dna Sequencing ◽  
Single Molecule ◽  
Zero Mode ◽  
Single Molecule Analysis

scholarly journals Zero-mode waveguides can be made better: fluorescence enhancement with rectangular aluminum nanoapertures from the visible to the deep ultraviolet

2020 ◽  
Vol 2 (9) ◽  
pp. 4153-4160 ◽  
Author(s):  
Mikhail Baibakov ◽  
Aleksandr Barulin ◽  
Prithu Roy ◽  
Jean-Benoît Claude ◽  
Satyajit Patra ◽  
...  
Keyword(s):  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Zero Mode ◽  
Metallic Films ◽  
Deep Ultraviolet ◽  
Single Molecule Analysis

Nanoapertures milled in metallic films called zero-mode waveguides (ZMWs) overcome the limitations of classical confocal microscopes by enabling single molecule analysis at micromolar concentrations with improved fluorescence brightness.

Zero-Mode Waveguides for Single-Molecule Analysis

2012 ◽  
Vol 41 (1) ◽  
pp. 269-293 ◽  
Author(s):  
Paul Zhu ◽  
Harold G. Craighead
Keyword(s):  
Single Molecule ◽  
Zero Mode ◽  
Single Molecule Analysis

Design and Fabrication of Linear-shaped Zero Mode Waveguides for Single Molecule Observation of Kinesin and Fluorescent ATP

2017 ◽  
Vol 137 (6) ◽  
pp. 159-164
Author(s):  
Kazuya Fujimoto ◽  
Yuki Morita ◽  
Ryota Iino ◽  
Michio Tomishige ◽  
Hirofumi Shintaku ◽  
...  
Keyword(s):  
Single Molecule ◽  
Zero Mode

Direct Read and Quantify Damage Nucleotide from an Oligonucleotide Using a Single Molecule Interface

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Meng-Yin Li ◽  
Jie Yang ◽  
Ya-Qian Wang ◽  
Xue-Yuan Wu ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Single Molecule ◽  
Genetic Diseases ◽  
High Sensitivity ◽  
Dna Lesions ◽  
Lesion Detection ◽  
The Novel ◽  
Structural Variations ◽  
Dna Lesion ◽  
Base Lesion

DNA lesion such as metholcytosine(<sup>m</sup>C), 8-OXO-guanine(<sup>O</sup>G), inosine(I) <i>etc</i> could cause the genetic diseases. Identification of the varieties of lesion bases are usually beyond the capability of conventional DNA sequencing which is mainly designed to discriminate four bases only. Therefore, lesion detection remain challenge due to the massive varieties and less distinguishable readouts for minor structural variations. Moreover, standard amplification and labelling hardly works in DNA lesions detection. Herein, we designed a single molecule interface from the mutant K238Q Aerolysin, whose confined sensing region shows the high compatible to capture and then directly convert each base lesion into distinguishable current readouts. Compared with previous single molecule sensing interface, the resolution of the K238Q Aerolysin nanopore is enhanced by 2-order. The novel K238Q could direct discriminate at least 3 types (<sup>m</sup>C, <sup>O</sup>G, I) lesions without lableing and quantify modification sites under mixed hetero-composition condition of oligonucleotide. Such nanopore could be further applied to diagnose genetic diseases at high sensitivity.

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