scholarly journals 1SBP-02 REAL-TIME MONITORING OF BIOMOLECULES IN ZERO-MODE WAVEGUIDES: DNA SEQUENCING AND BEYOND(1SBP Advanced Single Molecule Sequencing System,Symposium,The 51th Annual Meeting of the Biophysical Society of Japan)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S87
Author(s):  
Paul Peluso
Keyword(s):  
Dna Sequencing ◽  
Real Time ◽  
Annual Meeting ◽  
Single Molecule ◽  
Zero Mode ◽  
Real Time Monitoring ◽  
Single Molecule Sequencing ◽  
Biophysical Society

scholarly journals 2P-126 Real time imaging of tRNA dynamics with a zero-mode waveguides during translation(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S94-S95
Author(s):  
Sotaro Uemura ◽  
Yoshihiro Shimizu ◽  
Ryo Iizuka ◽  
Takuya Ueda ◽  
Rena Akahori ◽  
...  
Keyword(s):  
Real Time ◽  
Annual Meeting ◽  
Zero Mode ◽  
Real Time Imaging ◽  
Biophysical Society

A review of DNA sequencing techniques

2002 ◽  
Vol 35 (2) ◽  
pp. 169-200 ◽  
Author(s):  
Lilian T. C. França ◽  
Emanuel Carrilho ◽  
Tarso B. L. Kist
Keyword(s):  
Dna Sequencing ◽  
Real Time ◽  
Single Molecule ◽  
Fluorescent Dyes ◽  
Read Length ◽  
Chemical Methods ◽  
Single Molecule Sequencing ◽  
Exonuclease Digestion ◽  
Speed Accuracy ◽  
Sanger Method

1. Summary 1692. Introduction 1703. Sanger's method and other enzymic methods 1703.1 Random approach 1713.2 Direct approach 1713.3 Enzyme technology 1753.4 Sample preparation 1753.5 Labels and DNA labelling 1763.5.1 Radioisotopes 1763.5.2 Chemiluminescent detection 1763.5.3 Fluorescent dyes 1773.6 Fragment separation and analysis 1803.6.1 Electrophoresis 1803.6.2 Mass spectrometry – an alternative 1824. Maxam & Gilbert and other chemical methods 1835. Pyrosequencing – DNA sequencing in real time by the detection of released PPi 1876. Single molecule sequencing with exonuclease 1907. Conclusion 1928. Acknowledgements 1929. References 193The four best known DNA sequencing techniques are reviewed. Important practical issues covered are read-length, speed, accuracy, throughput, cost, as well as the automation of sample handling and preparation. The methods reviewed are: (i) the Sanger method and its most important variants (enzymic methods); (ii) the Maxam & Gilbert method and other chemical methods; (iii) the PyrosequencingTM method – DNA sequencing in real time by the detection of released pyrophosphate (PPi); and (iv) single molecule sequencing with exonuclease (exonuclease digestion of a single molecule composed of a single strand of fluorescently labelled deoxynucleotides). Each method is briefly described, the current literature is covered, advantages, disadvantages, and the most suitable applications of each method are discussed.

scholarly journals 1P307 Single molecule observation of actin polymerization in linear zero-mode waveguid(27. Bioimaging,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

2014 ◽  
Vol 54 (supplement1-2) ◽  
pp. S192
Author(s):  
Masamichi Yamamoto ◽  
Makoto Tsunoda ◽  
Shun Higano ◽  
Kotaro Okubo ◽  
Takashi Tanii ◽  
...  
Keyword(s):  
Annual Meeting ◽  
Single Molecule ◽  
Actin Polymerization ◽  
Zero Mode ◽  
Biophysical Society
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