scholarly journals Imaging Cellular Structures of Atherosclerotic Coronary Arteries Using Circumferentially Scanning Micro-Optical Coherence Tomography Fiber Probe Ex Vivo

IEEE Access ◽  
2018 ◽  
Vol 6 ◽  
pp. 62988-62994 ◽  
Author(s):  
Yuemei Luo ◽  
En Bo ◽  
Haitao Liang ◽  
Xianghong Wang ◽  
Xiaojun Yu ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Coronary Arteries ◽  
Ex Vivo ◽  
Cellular Structures ◽  
Optical Coherence ◽  
Fiber Probe

Fiber-needle Swept-source Optical Coherence Tomography System for the Identification of the Epidural Space in Piglets

2015 ◽  
Vol 122 (3) ◽  
pp. 585-594 ◽  
Author(s):  
Wen-Chuan Kuo ◽  
Meng-Chun Kao ◽  
Kuang-Yi Chang ◽  
Wei-Nung Teng ◽  
Mei-Yung Tsou ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Epidural Space ◽  
Ex Vivo ◽  
Optical Probe ◽  
Epidural Needle ◽  
Optical Coherence ◽  
Fiber Probe ◽  
Swept Source ◽  
Signal Intensities

Abstract Background: Epidural needle insertion is traditionally a blind technique whose success depends on the experience of the operator. The authors describe a novel method using a fiber-needle–based swept-source optical coherence tomography (SSOCT) to identify epidural space. Methods: An optical fiber probe was placed into a hollow 18-gauge Tuohy needle. It was then inserted by an experienced anesthesiologist to continuously construct a series of two-dimensional SSOCT images by mechanically rotating the optical probe. To quantify this observation, both the average SSOCT signal intensities and their diagnostic potentials were assessed. The insertions were performed three times into both the lumbar and thoracic regions of five pigs using a paramedian approach. Results: A side-looking SSOCT is constructed to create a visual image of the underlying structures. The image criteria for the identification of the epidural space from the outside region were generated by the analysis of a training set (n = 100) of ex vivo data. The SSOCT image criteria for in vivo epidural space identification are high sensitivity (0.867 to 0.965) and high specificity (0.838 to 0.935). The mean value of the average signal intensities exhibits statistically significant differences (P < 0.01) and a high discriminatory capacity (area under curve = 0.88) between the epidural space and the outside tissues. Conclusions: This is the first study to introduce a SSOCT fiber probe embedded in a standard epidural needle. The authors anticipate that this technique will reduce the occurrence of failed epidural blocks and other complications such as dural punctures.

scholarly journals A method to determine the radial compliance of porcine coronary arteries ex vivo via optical coherence tomography

2020 ◽  
Vol 6 (3) ◽  
pp. 48-51
Author(s):  
Christoph Brandt-Wunderlich ◽  
Franziska Bonin ◽  
Wolfram Schmidt ◽  
Niels Grabow ◽  
Klaus- Peter Schmitz ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Coronary Arteries ◽  
Vascular Tissue ◽  
Ex Vivo ◽  
Imaging Method ◽  
In Vitro Test ◽  
Optical Coherence ◽  
Porcine Coronary Artery ◽  
Vitro Test

AbstractOptical coherence tomography (OCT) as imaging method is widely used in ophthalmology, oncology and cardiology. For intravascular imaging the OCT is used for pre-interventional as well as post-procedural assessments. Within the current study a test setup for ex vivo determination of the compliance of porcine coronary arteries via OCT is described. Diameter measurements based on OCT imaging were performed during consecutive pressurization of a porcine coronary artery from 40 to 200 mmHg in a physiological environment. The test results indicate that the radial compliance depends on the specific segment of the artery as well as the pressure range considered. The revealed compliance data can be used for numerical simulations of the vascular tissue as well as for optimization of in vitro test setups for pulsatile testing of vascular implants.

scholarly journals Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1445-1456 ◽  
Author(s):  
Fabian Placzek ◽  
Eliana Cordero Bautista ◽  
Simon Kretschmer ◽  
Lara M. Wurster ◽  
Florian Knorr ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Imaging System ◽  
Ex Vivo ◽  
Large Field ◽  
Optical Coherence ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Muscle Invasive

Characterization of bladder biopsies, using a combined fiber optic probe-based optical coherence tomography and Raman spectroscopy imaging system that allows a large field-of-view imaging and detection and grading of cancerous bladder lesions.

Ex Vivo assessment of competent strut coverage after coronary stenting by optical coherence tomography

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
R Bhoite ◽  
H Jinnouchi ◽  
F Otsuka ◽  
Y Sato ◽  
A Sakamoto ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Predictive Value ◽  
Ex Vivo ◽  
High Sensitivity ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Neointimal Thickness ◽  
Stent Strut ◽  
Oct Imaging ◽  
Definition Of

Abstract Background In many studies, struts coverage is defined as >0 mm of tissue overlying the stent struts by optical coherence tomography (OCT). However, this definition has never been validated using histology as the “gold standard”. The present study sought to assess the appropriate cut-off value of neointimal thickness of stent strut coverage by OCT using histology. Methods OCT imaging was performed on 39 human coronary arteries with stents from 25 patients at autopsy. A total of 165 cross-sectional images from 46 stents were co-registered with histology. The optimal cut-off value of strut coverage by OCT was determined. Strut coverage by histology was defined as endothelial cells with at least underlying two layers of smooth muscle cells. Considering the resolution of OCT is 10–20 μm, 3 different cut-off values (i.e. at ≥20, ≥40, and ≥60 μm) were assessed. Results A total of 2235 struts were evaluated by histology. Eventually, 1216 struts which were well-matched struts were analyzed in this study. By histology, uncovered struts were observed in 160 struts and covered struts were observed in 1056 struts. The broadly used definition of OCT-coverage which does not consider neointimal thickness yielded a poor specificity of 37.5% and high sensitivity 100%. Of 3 cut-off values, the cut-off value of >40 μm was more accurate as compared to >20 and >60 mm [sensitivity (99.3%), specificity (91.0%), positive predictive value (98.6%), and negative predictive value (95.6%)] Conclusion The most accurate cut-off value was ≥40 μm neointimal thickness by OCT in order to identify stent strut coverage validated by histology. Funding Acknowledgement Type of funding source: None

Transscleral optical coherence tomography?An experimental study in ex-vivo human eyes

2002 ◽  
Vol 30 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Hans Hoerauf ◽  
J�rg Winkler ◽  
Christian Scholz ◽  
Christopher Wirbelauer ◽  
Roswitha S. Gordes ◽  
...  
Keyword(s):  
Experimental Study ◽  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Human Eyes
Sign in / Sign up

Export Citation Format

Share Document