Assessing ex vivo dental biofilms and in vivo composite restorations using cross-polarization optical coherence tomography

2012 ◽  
Author(s):  
R. Jones ◽  
C. Aparicio ◽  
R. Chityala ◽  
R. Chen ◽  
A. Fok ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Cross Polarization ◽  
Composite Restorations

scholarly journals Imaging in vivo secondary caries and ex vivo dental biofilms using cross-polarization optical coherence tomography

2012 ◽  
Vol 28 (7) ◽  
pp. 792-800 ◽  
Author(s):  
Pat Lenton ◽  
Joel Rudney ◽  
Ruoqiong Chen ◽  
Alex Fok ◽  
Conrado Aparicio ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Cross Polarization ◽  
Secondary Caries

scholarly journals Polarization sensitive optical coherence tomography with single input for imaging depth-resolved collagen organizations

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Peijun Tang ◽  
Mitchell A. Kirby ◽  
Nhan Le ◽  
Yuandong Li ◽  
Nicole Zeinstra ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Structural Integrity ◽  
Ex Vivo ◽  
Polarization State ◽  
Transmission Model ◽  
Optical Coherence ◽  
Healthy Human ◽  
Collagen Organization ◽  
Single Input

AbstractCollagen organization plays an important role in maintaining structural integrity and determining tissue function. Polarization-sensitive optical coherence tomography (PSOCT) is a promising noninvasive three-dimensional imaging tool for mapping collagen organization in vivo. While PSOCT systems with multiple polarization inputs have demonstrated the ability to visualize depth-resolved collagen organization, systems, which use a single input polarization state have not yet demonstrated sufficient reconstruction quality. Herein we describe a PSOCT based polarization state transmission model that reveals the depth-dependent polarization state evolution of light backscattered within a birefringent sample. Based on this model, we propose a polarization state tracing method that relies on a discrete differential geometric analysis of the evolution of the polarization state in depth along the Poincare sphere for depth-resolved birefringent imaging using only one single input polarization state. We demonstrate the ability of this method to visualize depth-resolved myocardial architecture in both healthy and infarcted rodent hearts (ex vivo) and collagen structures responsible for skin tension lines at various anatomical locations on the face of a healthy human volunteer (in vivo).

scholarly journals Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents

2020 ◽  
Vol 245 (18) ◽  
pp. 1629-1636
Author(s):  
Ruiming Kong ◽  
Wenjuan Wu ◽  
Rui Qiu ◽  
Lei Gao ◽  
Fengxian Du ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Light Attenuation ◽  
Anterior Segment ◽  
Posterior Segment ◽  
Glycerol Solution ◽  
Optical Coherence ◽  
Optical Clearing ◽  
Imaging Depth

Optical coherence tomography has become an indispensable diagnostic tool in ophthalmology for imaging the retina and the anterior segment of the eye. However, the imaging depth of optical coherence tomography is limited by light attenuation in tissues due to optical scattering and absorption. In this study of rabbit eye both ex vivo and in vivo, optical coherence tomography imaging depth of the anterior and posterior segments of the eye was extended by using optical clearing agents to reduce multiple scattering. The sclera, the iris, and the ciliary body were clearly visualized by direct application of glycerol at an incision on the conjunctiva, and the posterior boundary of sclera and even the deeper tissues were detected by submerging the posterior segment of eye in glycerol solution ex vivo or by retro-bulbar injection of glycerol in vivo. The ex vivo rabbit eyes recovered to their original state in 60 s after saline-wash treatment, and normal optical coherence tomography images of the posterior segment of the sample eyes proved the self-recovery of in vivo performance. Signal intensities of optical coherence tomography images obtained before and after glycerol treatment were compared to analysis of the effect of optical clearing. To the best of our knowledge, this is the first study for imaging depth extension of optical coherence tomography in both the anterior and posterior segments of eye by using optical clearing agents.

scholarly journals Comparison of optical coherence tomography and high frequency ultrasound imaging in mice for the assessment of skin morphology and intradermal volumes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kornelia Schuetzenberger ◽  
Martin Pfister ◽  
Alina Messner ◽  
Vanessa Froehlich ◽  
Gerhard Garhoefer ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Frequency ◽  
Ex Vivo ◽  
Clinical Settings ◽  
Optical Coherence ◽  
High Frequency Ultrasound ◽  
Skin Morphology ◽  
Frequency Ultrasound ◽  
Good Agreement

Abstract Optical coherence tomography (OCT) and high-frequency ultrasound (HFUS), two established imaging modalities in the field of dermatology, were evaluated and compared regarding their applicability for visualization of skin tissue morphology and quantification of murine intradermal structures. The accuracy and reproducibility of both methods were assessed ex vivo and in vivo using a standardized model for intradermal volumes based on injected soft tissue fillers. OCT revealed greater detail in skin morphology, allowing for detection of single layers due to the superior resolution. Volumetric data measured by OCT (7.9 ± 0.3 μl) and HFUS (7.7 ± 0.5 μl) were in good agreement and revealed a high accuracy when compared to the injected volume of 7.98 ± 0.8 µl. In vivo, OCT provided a higher precision (relative SD: 26% OCT vs. 42% HFUS) for the quantification of intradermal structures, whereas HFUS offered increased penetration depth enabling the visualization of deeper structures. A combination of both imaging technologies might be valuable for tumor assessments or other dermal pathologies in clinical settings.

scholarly journals Differential diagnosis of human bladder mucosa pathologies in vivo with cross-polarization optical coherence tomography

2015 ◽  
Vol 6 (4) ◽  
pp. 1464 ◽  
Author(s):  
Elena Kiseleva ◽  
Mikhail Kirillin ◽  
Felix Feldchtein ◽  
Alex Vitkin ◽  
Ekaterina Sergeeva ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Differential Diagnosis ◽  
Optical Coherence ◽  
Cross Polarization ◽  
Bladder Mucosa ◽  
Human Bladder

scholarly journals Polarization-sensitive optical coherence tomography monitoring of percutaneous radiofrequency ablation in left atrium of living swine

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaowei Zhao ◽  
Ohad Ziv ◽  
Reza Mohammadpour ◽  
Benjamin Crosby ◽  
Walter J. Hoyt ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Radiofrequency Ablation ◽  
Left Atrium ◽  
Real Time ◽  
Wall Thickness ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Percutaneous Radiofrequency Ablation ◽  
Cardiac Wall

AbstractRadiofrequency ablation (RFA) is commonly used to treat atrial fibrillation (AF). However, the outcome is often compromised due to the lack of direct real-time feedback to assess lesion transmurality. In this work, we evaluated the ability of polarization-sensitive optical coherence tomography (PSOCT) to measure cardiac wall thickness and assess RF lesion transmurality during left atrium (LA) RFA procedures. Quantitative transmural lesion criteria using PSOCT images were determined ex vivo using an integrated PSOCT-RFA catheter and fresh swine hearts. LA wall thickness of living swine was measured with PSOCT and validated with a micrometer after harvesting the heart. A total of 38 point lesions were created in the LA of 5 living swine with the integrated PSOCT-RFA catheter using standard clinical RFA procedures. For all lesions with analyzable PSOCT images, lesion transmurality was assessed with a sensitivity of 89% (17 of 19 tested positive) and a specificity of 100% (5 of 5 tested negative) using the quantitative transmural criteria. This is the first report of using PSOCT to assess LA RFA lesion transmurality in vivo. The results indicate that PSOCT may potentially provide direct real-time feedback for LA wall thickness and lesion transmurality.

A comparative in-vivo study of the female urethra with cross-polarization optical coherence tomography using two types of endoscopic probes

2021 ◽  
Author(s):  
Elena B. Kiseleva ◽  
Pavel A. Shilyagin ◽  
Vasily V. Vlasov ◽  
Anton S. Kuyarov ◽  
Muhammat A. Molvi ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
In Vivo Study ◽  
Optical Coherence ◽  
Cross Polarization ◽  
Female Urethra
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