scholarly journals Variation in Expression of the HECT E3 Ligase UPL3 Modulates LEC2 Levels, Seed Size, and Crop Yields in Brassica napus

2019 ◽  
Vol 31 (10) ◽  
pp. 2370-2385 ◽  
Author(s):  
Charlotte Miller ◽  
Rachel Wells ◽  
Neil McKenzie ◽  
Martin Trick ◽  
Joshua Ball ◽  
...  
2018 ◽  
Author(s):  
Charlotte Miller ◽  
Rachel Wells ◽  
Neil McKenzie ◽  
Martin Trick ◽  
Joshua Ball ◽  
...  

AbstractIdentifying genetic variation that increases crop yields is a primary objective in plant breeding. We have used association analyses of Brassica napus (oilseed rape/canola) accessions to identify variation in the expression of a HECT E3 ligase gene, BnaUPL3.C03, that influences seed size and final yield. We establish a mechanism in which UPL3 mediates the proteasomal degradation of LEC2, a master regulator of seed maturation. Reduced UPL3 expression increases LEC2 protein levels and prolongs expression of lipid biosynthetic genes and seed maturation. Natural variation in BnaUPL3.C03 expression has not yet been exploited in current Brassica napus breeding lines and can therefore be used as a new approach to maximize future yields in this important oil crop.


2014 ◽  
Vol 14 (2) ◽  
pp. 152-159
Author(s):  
Wuye Ria Andayanie ◽  
Praptiningsih Gamawati Adinurani

Soybean lines selection of F4 population resistant  to soybean mosaic disease (Soybean mosaic virus) with high yield.  The soybean breeding program is usually not purposedly done for resistance to Soybean mosaic virus (SMV) but rather for crop yields. The experiment was aimed to obtain soybean lines of F4 population resistant to soybean mosaic disease with high yield.  F2-F4 plants that have been inoculated with the T isolate of SMV one week after planting were selected by the pedigree  in the screen house. The result indicated eight  F4 populations (Wilis x L. Temanggung; Wilis x L. Jombang; Wilis x Pangrango; Wilis x PI 200485;  Gepak Kuning x L. Jombang; Gepak Kuning x L. Temanggung; Gepak Kuning x Malabar; Gepak Kuning x PI 200485) produced medium seed size (from 9.84-10.26 g 100/seeds).  Gepak Kuning x Mlg 3288  showed more resistant than Gepak Kuning x PI 200485. The seed produced by Gepak Kuning x PI 200485 was 1.97 ton/ha. There were no F4 populations that had higher yield and bigger seed size than Gepak Kuning x PI 200485 even though they were  moderately resistant to SMV. Therefore, these lines of Gepak Kuning x Mlg 3288 and Gepak  Kuning x  PI 200485 might provide exellent sources to develop a new variety that resistant to SMV and of high yield.


1996 ◽  
Vol 10 (4) ◽  
pp. 907-913 ◽  
Author(s):  
Kenneth J. Kirkland

The comparison of incorporation vs no incorporation on a mid-October application of the granular formulation of trifluralin to control infestations of wild oat and green foxtail was evaluated in spring barley, wheat, and canola in west central Saskatchewan over a 3 yr period. Incorporation treatments consisted of: no incorporation, one incorporation in fall, one incorporation in fall and a second in spring, one incorporation in fall and two additional incorporations in spring. All treatments eliminated green foxtail. In all three crops wild oat panicle counts were equivalent from incorporated and non-incorporated trifluralin. Wild oat fresh weights in crops grown on stubble were similar for incorporated and non-incorporated trifluralin. In fallow crops, wild oat fresh weight reductions were greater in three of nine site years with incorporation. There was little difference in crop yields from incorporated and non-incorporated trifluralin.


2001 ◽  
Vol 81 (1) ◽  
pp. 59-60 ◽  
Author(s):  
L. J. Lewis ◽  
D. L. Woods ◽  
B. F. Cheng

Long pod character from a summer Brassica napus L. line was introgressed into turnip rape B. rapa L. cultivar AC Sunshine resulting in progeny lines with significantly longer pods and larger seed size than those of AC Sunshine, but producing no yield advantage. Key words: Brassica rapa, Brassica napus, pod length, summer turnip rape, spring rape, interspecific hybridisation


1991 ◽  
Vol 71 (2) ◽  
pp. 397-404 ◽  
Author(s):  
R. P. Bodnaryk ◽  
R. J. Lamb

Seedlings of Brassica napus L. 'Westar' or Sinapis alba L. 'Ochre' grown from seeds of various size were offered to flea beetles, Phyllotreta cruciferae (Goeze) in feeding tests for damage assessment. The proportion of cotyledon area damaged in both species was highest for seedlings grown from small seeds in choice and no-choice feeding tests. The proportion of seedlings killed by flea beetle feeding was also highest in seedlings from small seeds, an effect that was especially pronounced at high beetle densities (10/seedling) where 100% of seedlings from small seeds of B. napus were killed compared to 28.3% of seedlings from big seeds. For S. alba, at 20 beetles/seedling, 45.4% of seedlings from small seeds were killed compared to only 9.1% of seedlings from big seeds. Seedlings grown from big seeds of S. alba tolerated low levels of damage caused by flea beetle feeding or by scissors. No evidence for tolerance was obtained for seedlings from small seeds of S. alba or for seedlings from big or small seeds of B. napus. "Big seeds" appears to be a desirable trait that enhances crucifer seedling resistance to flea beetle attack and results in increased seedling survival. Key words: Brassica napus 'Westar', Sinapis alba 'Ochre', Phyllotreta cruciferae, flea beetle, seed size, resistance


PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39705 ◽  
Author(s):  
Yong-bo Liu ◽  
Zhi-xi Tang ◽  
Henri Darmency ◽  
C. Neal Stewart ◽  
Kun Di ◽  
...  

2010 ◽  
Vol 90 (2) ◽  
pp. 145-152 ◽  
Author(s):  
B. Irvine ◽  
G P Lafond

Compared with direct harvesting, windrowing canola (Brassica napus L.) crop reduces maturation time and seed losses caused by shattering; however, windrows are prone to wind damage. While direct harvesting canola may reduce costs and lower green seed content, new technologies are required to effectively reduce shattering losses. Pushing is a potential replacement for swathing where pod movement is restricted by mechanically lodging the crop and letting the crop mature while still attached to the root system. This system purports to limit shattering and improve seed quality. Trials were conducted over a 3-yr period in western Canada to determine the impact of pushing canola relative to windrowing. Field-scale trials showed that yield and oil content typically did not differ between canola pushed and windrowed on the same date. In plot trials conducted at Brandon and Indian Head, crop yields were not decreased nor were green seed numbers increased by early pushing. It was observed that pushing worked best when crop stand and growth were good as well as when canola was pushed at or before 30-40% of the seeds have changed color and therefore are physiologically mature. While further work is required to identify the earliest time at which a crop can be pushed without a negative impact on yield or quality, the current trials indicate that with canola, pushing could occur at the start of seed color change without any negative impacts on grain yield and oil quality. Key words: Canola, windrowing, pushing, harvest, yield, seed size, Brassica napus L.


2006 ◽  
Vol 86 (4) ◽  
pp. 1059-1070 ◽  
Author(s):  
P. R. Miller ◽  
S. A. Brandt ◽  
C. L. McDonald ◽  
J. Waddington

We compared the effects of spring seeding date on stand density, crop growth, seed yield, water-use-efficiency, and grain quality of three pulse crops [chickpea (Cicer arietinum L.), lentil (Lens culinaris Medik.) and pea (Pisum sativum L.)] grown at Scott and Swift Current, SK, during 1993 to 1997. Wheat (Triticum aestivum L. emend. Thell.) was used as a reference crop. Seeding date did not meaningfully affect plant density for chickpea, lentil or pea. Crop growth stage related to thermal time resulted in robust prediction equations for chickpea, lentil and pea (R2 = 0.87 to 0.98). When seeding was delayed 2 wk, the crop response was inconsistent. When seeding was delayed by 4 wk, consistent, mainly negative, effects on crop parameters occurred due to the exacerbation of summer drought stress. Pulse crop yields were more negatively affected by delayed seeding than were wheat yields. When seeding of chickpea, lentil, and pea was delayed 4 wk, seed yield decreased at the majority of site-years. When yield reductions occurred, the average reductions were 44, 38, and 31%, for the respective species. For chickpea and lentil, seeding delays of 2 and 4 wk decreased seed size at the majority of site-years with seed size reductions averaging 10% when they occurred. In the semiarid Northern Great Plains (NGP), chickpea, lentil, and pea should be seeded before spring wheat due to a greater risk of loss of yield and quality. Key words: Chickpea, growth stage, lentil, northern great plains, pea, seeding date


2021 ◽  
Author(s):  
Ying Ju Li ◽  
Yang Yu ◽  
Xiuying Liu ◽  
Xian Sheng Zhang ◽  
Ying Hua Su

AbstractSeed size is a major factor determining crop yields that is controlled through the coordinated development of maternal and zygotic tissues. Here, we identified Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 (MEE45) as a B3 transcription factor that controls cell proliferation and maternally regulates seed size through its transcriptional activation of AINTEGUMENTA (ANT) and its downstream control of auxin biosynthesis in the ovule integument. After characterizing reduced seed and organ size phenotypes in mee45 mutants and finding that overexpression of MEE45 causes oversized seeds, we discovered that the MEE45 protein can bind to the promoter region of the ANT locus and positively regulate its transcription. ANT in-turn activates the expression of auxin biosynthetic genes (e.g. YUCCA4) in the ovule integument. Our results thus illustrate mechanisms underlying maternal tissue-mediated regulation of seed size and suggest that MEE45 and its downstream components can be harnessed to develop higher-yielding crop varieties.


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