scholarly journals SlMYC1 Regulates Type VI Glandular Trichome Formation and Terpene Biosynthesis in Tomato Glandular Cells

2018 ◽  
Vol 30 (12) ◽  
pp. 2988-3005 ◽  
Author(s):  
Jiesen Xu ◽  
Zeger O. van Herwijnen ◽  
Dörthe B. Dräger ◽  
Chun Sui ◽  
Michel A. Haring ◽  
...  
Keyword(s):  
Glandular Trichome ◽  
Terpene Biosynthesis ◽  
Trichome Formation ◽  
Glandular Cells

scholarly journals Single Wavelengths of LED Light Supplement Promote the Biosynthesis of Major Cyclic Monoterpenes in Japanese Mint

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1420
Author(s):  
Takahiro Ueda ◽  
Miki Murata ◽  
Ken Yokawa
Keyword(s):  
Blue Light ◽  
Solid Phase ◽  
Glandular Trichomes ◽  
Red Light ◽  
Glandular Trichome ◽  
Pulse Amplitude ◽  
Light Treatment ◽  
Led Light ◽  
Trichome Formation ◽  
Japanese Mint

Environmental light conditions influence the biosynthesis of monoterpenes in the mint plant. Cyclic terpenes, such as menthol, menthone, pulegone, and menthofuran, are major odor components synthesized in mint leaves. However, it is unclear how light for cultivation affects the contents of these compounds. Artificial lighting using light-emitting diodes (LEDs) for plant cultivation has the advantage of preferential wavelength control. Here, we monitored monoterpene contents in hydroponically cultivated Japanese mint leaves under blue, red, or far-red wavelengths of LED light supplements. Volatile cyclic monoterpenes, pulegone, menthone, menthol, and menthofuran were quantified using the head-space solid phase microextraction method. As a result, all light wavelengths promoted the biosynthesis of the compounds. Remarkably, two weeks of blue-light supplement increased all compounds: pulegone (362% increase compared to the control), menthofuran (285%), menthone (223%), and menthol (389%). Red light slightly promoted pulegone (256%), menthofuran (178%), and menthol (197%). Interestingly, the accumulation of menthone (229%) or menthofuran (339%) was observed with far-red light treatment. The quantification of glandular trichomes density revealed that no increase under light supplement was confirmed. Blue light treatment even suppressed the glandular trichome formation. No promotion of photosynthesis was observed by pulse-amplitude-modulation (PAM) fluorometry. The present result indicates that light supplements directly promoted the biosynthetic pathways of cyclic monoterpenes.

scholarly journals Genetic basis for glandular trichome formation in cotton

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Dan Ma ◽  
Yan Hu ◽  
Changqing Yang ◽  
Bingliang Liu ◽  
Lei Fang ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Glandular Trichome ◽  
Trichome Formation

Hair (H) interacts with SlZFP8-like to regulate the initiation and elongation of trichomes by modulating SlZFP6 expression in tomato

2021 ◽  
Author(s):  
Fangyan Zheng ◽  
Long Cui ◽  
Changxing Li ◽  
Qingmin Xie ◽  
Guo Ai ◽  
...  
Keyword(s):  
Zinc Finger Protein ◽  
Glandular Trichome ◽  
Type I ◽  
Trichome Density ◽  
Trichome Formation ◽  
Finger Protein ◽  
Biochemical Assays ◽  
Direct Target ◽  
Glandular Structures ◽  
Close Homolog

Abstract Trichomes are specialized glandular or non-glandular structures that provide physical or chemical protection against insect and pathogens attack. Trichomes in Arabidopsis, as typical non-glandular structures, have been extensively studied. However, the molecular mechanism underlying glandular trichome formation and elongation still remains largely unknown. We previously demonstrated that Hair (H) is essential for the formation of type I and type VI trichomes. Here, we found that overexpression of H increased the density and length of tomato trichomes. We revealed that H physically interacts with its close homolog SlZFP8-like (SlZFP8L) and SlZFP8L also directly interacts with Woolly (Wo) by biochemical assays. SlZFP8L overexpression plants showed increased trichome density and length. We further found that the expression of SlZFP6, encoding a C2H2 zinc finger protein, is positively regulated by H. We identified that SlZFP6, is a direct target of H through ChIP-qPCR, Y1H, and LUC assays. Similar to H and SlZFP8L, the overexpression of SlZFP6 also increased the density and length of tomato trichomes. Taken together, our results suggest that H interacts with SlZFP8-like to regulate the initiation and elongation of trichomes by modulating SlZFP6 expression in tomato.

Genetic Control of Peltate Glandular Trichome Formation inPerilla frutescens

Planta Medica ◽  
1992 ◽  
Vol 58 (02) ◽  
pp. 188-191 ◽  
Author(s):  
Atsushi Nishizawa ◽  
Gisho Honda ◽  
Yoko Kobayashi ◽  
Mamoru Tabata
Keyword(s):  
Genetic Control ◽  
Glandular Trichome ◽  
Trichome Formation

scholarly journals Hairiness Gene Regulated Multicellular, Non-Glandular Trichome Formation in Pepper Species

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinqiu Liu ◽  
Haoran Wang ◽  
Mengmeng Liu ◽  
Jinkui Liu ◽  
Sujun Liu ◽  
...  
Keyword(s):  
Zinc Finger Protein ◽  
Mechanistic Model ◽  
Glandular Trichomes ◽  
Glandular Trichome ◽  
Type I ◽  
Virus Induced Gene Silencing ◽  
Promoter Sequences ◽  
Trichome Formation ◽  
Defensive Role ◽  
Multicellular Trichome

Trichomes are unicellular or multicellular epidermal structures that play a defensive role against environmental stresses. Although unicellular trichomes have been extensively studied as a mechanistic model, the genes involved in multicellular trichome formation are not well understood. In this study, we first classified the trichome morphology structures in Capsicum species using 280 diverse peppers. We cloned a key gene (Hairiness) on chromosome 10, which mainly controlled the formation of multicellular non-glandular trichomes (types II, III, and V). Hairiness encodes a Cys2-His2 zinc-finger protein, and virus-induced gene silencing of the gene resulted in a hairless phenotype. Differential expression of Hairiness between the hairiness and hairless lines was due to variations in promoter sequences. Transgenic experiments verified the hypothesis that the promoter of Hairiness in the hairless line had extremely low activity causing a hairless phenotype. Hair controlled the formation of type I glandular trichomes in tomatoes, which was due to nucleotide differences. Taken together, our findings suggest that the regulation of multicellular trichome formation might have similar pathways, but the gene could perform slightly different functions in crops.

NtCycB2 negatively regulates tobacco glandular trichome formation, exudate accumulation, and aphid resistance

2021 ◽  
Author(s):  
Zhaojun Wang ◽  
Xiaoxiao Yan ◽  
Hongying Zhang ◽  
Ying Meng ◽  
Yang Pan ◽  
...  
Keyword(s):  
Glandular Trichome ◽  
Aphid Resistance ◽  
Trichome Formation

Occurrence and Development of a Dorsal Gland in the Leaves of Twelve Cultivars of Ficus benjamina L. (Weeping Fig)

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 537b-537
Author(s):  
Svoboda V. Pennisi ◽  
Dennis B. McConnell ◽  
Richard W. Henley
Keyword(s):  
Leaf Size ◽  
Dark Spot ◽  
Ficus Benjamina ◽  
Axis Parallel ◽  
Glandular Cells ◽  
Phenolic Substances ◽  
Dark Color ◽  
Columnar Cells ◽  
Dorsal Gland ◽  
Colorimetric Reaction

Ficus benjamina plants are an integral part of most modern interior landscapes. Reports from growers and interiorscape managers have drawn attention to a specific problem related to large F. benjamina plants, namely the occurrence of a dark oval spot on the abaxial surface of the leaf base. Twelve cultivars of F. benjamina were examined: Christine, Citation, Florida Spire, Kelly, Kiki, Midnight, Monique, Stacey, Wintergreen, Dwarf Nikita, Spearmint, and Starlight. Anatomically, the dorsal gland consisted of one to several layers of densely stained, columnar cells. Positive colorimetric reaction for phenolics was obtained in the glandular cells. Developmentally, the gland cells could not be distinguished from the regular epidermal cells until ≈30% of final leaf size was reached. The cells of the outermost glandular layer changed shape from rectangular with long axis parallel to the leaf surface to elongate with long axis perpendicular to the surface. In a mature leaf, the thickness of the glandular layer was between 20 and 30 μm. Externally, at this stage, no dark spot, indicative of the gland's location, could be observed. In older leaves, however, an accumulation of phenolic substances led to appearance of dorsal dark spot. All cultivars possessed glandular layer. However, this area did not darken in all cultivars; Christine, Citation, Florida Spire, Kelly, Kiki, and Stacey developed small dark spots, while Dwarf Nikita and Starlight had numerous, well-pronounced glandular regions. This study showed that the dark spots in F. benjamina cultivars were a normal morphological feature. Although the gland was present in every cultivar, only a few cultivars developed a dark color.

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